The role of base excision repair in pathogenesis of breast cancer in the Polish population

Abstract

Breast cancer (BC) is leading type of cancer among group of women, which determines almost 23% of invasive cancers. It has been reported repeatedly that the level of oxidative stress is higher for BC in comparison to cancer-free woman. The goal of the present study was to evaluate the role of base excision repair (BER) pathway in the development of BC. One-hundred seventy-one women with confirmed BC and 222 healthy controls were enrolled in presented study. The level of oxidative DNA damage and the kinetic of their repair were analyzed by the modified alkaline comet assay. The efficiency of BER pathway was evaluated by BER assay. The presence of the 326Cys/Cys genotype and 326Cys allele of OGG1 gene and the 324His/His of MUTYH gene are associated with increased risk of BC development. Moreover, correlation between clinical parameter with selected genes has shown increased risk of BC progression. The survival analysis has shown a significant lower DFS for individuals with the 762Ala/Ala genotype compared to 762Val/Vla carriers and the 762Val/Ala genotype in relation to concomitant chemotherapy and radiotherapy. In subgroup of patients with alone chemotherapy and alone radiotherapy, the 762Val/Val genotype was significantly associated with lower overall survival. Furthermore, we also elevated the level of basal and oxidative DNA damage in a group of patients with BC in relation to healthy controls. We also observed the difference in effectiveness of DNA damage repair. The results of present studies suggested the important role of BER pathway in BC development. © 2015 Wiley Periodicals, Inc.

RETRACTED ARTICLE: Long non-coding RNA SATB2-AS1 inhibits microRNA-155-3p to suppress breast cancer cell growth by promoting breast cancer metastasis suppressor 1-like

“…However, there is still an elevating mortality in some particular countries [ 4 ]. It has been demonstrated that both environmental and genetic factors contribute to the risk of BC [ 5 ]. In addition, several clinicopathological parameters have been introduced in BC management, including clinical stages, histological grade, histological type and lymph node metastasis (LNM).…”

DNA Repair Deficiency in Breast Cancer: Opportunities for Immunotherapy

“…This method of repair involves the removal of damaged bases form the double helix and the excision of the damaged section from the DNA structure [19]. Single nucleotide polymorphisms (SNPs) in members of the base excision repair pathway, XRCC1 and APE1, have been reported as contributing to increased risk of breast cancer, although population studies have not yielded consistent results [20, 21].…”

The overexpression of DNA repair genes in invasive ductal and lobular breast carcinomas: Insights on individual variations and precision medicine

“…Single nucleotide polymorphisms (SNPs) in the XRCC1 and APE1 base excision repair (BER) genes have also been reported to increase the risk of breast cancer on the level of individuals. However, no population specific data was reported [19,20]. Nucleotide excision repair (NER) deficiency has also been associated with early stage breast cancer.…”