The response of phenylalanine ammonia‐lyase, polyphenol oxidase and phenols to cold stress in the olive tree (<i>Olea europaea</i> L. cv. Picual)

Ortega-García, Francisca; Peragón, Juan

doi:10.1002/jsfa.3625

Cited by 89 publications

(47 citation statements)

References 51 publications

(61 reference statements)

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“…PPO enzyme also catalyses the oxidation of o diphe nols to o diquinones, as well as the hydroxylation of monophenols. PPO is important in the response of plants against freezing stress and it can help avoid seri ous oxidative damage induced by freezing [6].…”

Section: Discussionmentioning

confidence: 99%

“…Then, POD and CAT convert H 2 O 2 into H 2 O and O 2 , whereas APX decomposes H 2 O 2 by oxidation of co substrates, such as phenolic compounds and/or antioxidants [5]. In addition, polyphenol oxidase (PPO) catalyzes the oxidation of o diphenols to o diquinones, as well as the hydroxylation of monophenols [6]. PPO is also an important enzyme in the response of plants against freezing stress, and it can help to avoid serious oxidative damage induced by freezing [6].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, polyphenol oxidase (PPO) catalyzes the oxidation of o diphenols to o diquinones, as well as the hydroxylation of monophenols [6]. PPO is also an important enzyme in the response of plants against freezing stress, and it can help to avoid serious oxidative damage induced by freezing [6]. In order to accommodate the oxidative stresses, it is crucial that plants maintain the activities of these antioxidant enzymes.…”

Section: Introductionmentioning

confidence: 99%

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The physiological and biochemical responses of eastern purple coneflower to freezing stress

Asadi‐Sanam

Pirdashti

Hashempour

et al. 2015

Russ J Plant Physiol

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The freezing hardiness (expressed as LT 50 ) as well as changes in the antioxidant enzymes activity, total protein and lipid peroxidation (MDA content), total phenolic and flavonoid content, antioxidant capacity, chlorophyll fluorescence (F v /F m ) of Echinacea purpurea (L.) Moench were investigated. Five month old purple coneflower seedlings were kept at 4°C for two weeks to induce cold acclimation. The acclimated seedlings were treated with freezing temperatures (0, -4, -8, -12, -16, and -20°C) for 6 h. The unfrozen control plants were kept at 4°C. The results with lowering freezing temperatures showed a sharp increase of ion leakage and MDA content at -20°C as compared to the nonfreezing temperature. Exposing seedlings to freezing temperatures were accompanied by decreasing dark adapted chlorophyll fluorescence (F v /F m ). Freezing stress significantly reduced superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) activity of seedling leaf except at 0°C. With lowering freezing temperature, peroxidase (POD) and polyphenol oxidase (PPO) activity showed a sharp decline to -20°C. Furthermore, total protein and antioxidant capacity of Echinacea leaves were declined significantly after exposure to freezing temperature, and thereafter reached to the highest at ⎯8°C. Total phenolic content of freezing treated seedlings was significantly lower than that of the nonfreezing seed lings. Total flavonoid content increased significantly with lowering freezing temperatures. It was found that per centage of freezing injury closely correlated to antioxidant enzymes activity (POD and PPO; r = -0.93) and F v /F m ratio (r = -0.77). Based on our results, the freezing tolerance (LT 50 ) of Echinacea seedlings under artifi cially simulated freezing stress in the laboratory was -7°C.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The physiological and biochemical responses of eastern purple coneflower to freezing stress

Asadi‐Sanam

Pirdashti

Hashempour

et al. 2015

Russ J Plant Physiol

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“…Currently, interesting results to quantify cold tolerance have come from the study of some antioxidant enzymes (Solecka and Kacperska 2003;Cansev et al 2009;Ortega-García and Peragón 2009). A significant increase in the activity of CAT, APX and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase as well as in dehydrin protein levels in cold-acclimatized olive trees was reported ).…”

Section: Introductionmentioning

confidence: 95%

Olive (Olea europaea L.) freezing tolerance related to antioxidant enzymes activity during cold acclimation and non acclimation

et al. 2014

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The changes in the antioxidant enzymes activity, total protein and proline content and their correlations with freezing tolerance (FT) (expressed as LT 50 ) were investigated at 11 different olive cultivars at cold-acclimation (CA, in February) and non-acclimation (NA, in August) stages. Leaf samples were collected from each cultivar and were divided into two groups. The first group was immediately frozen in liquid nitrogen for further biochemical analysis. The second ones was subjected to different freezing temperatures (-5, -10, -15 and -20°C) for 10 h, in order to determine their FT. The unfrozen control samples were kept at 4°C. The results showed that Fishomi, Mission and Shengeh were the most freezing tolerant among other cultivars. In contrast, Zard, Manzanilla and Amigdalolia were the most sensitive ones. The cold acclimation enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), polyphenol oxidase (PPO) and total protein content. However, proline content and phenylalanine ammonia-lyase (PAL) activity did not change or even decreased slightly at CA stage, compare to those samples at NA stage. It was found that LT 50 to be closely correlated to POD, CAT, and PPO activity at CA and NA stages. Overall, higher leaf POD, CAT, and PPO activity could be used as important selection criteria in screening tolerant olive cultivars for cold zone climatic.

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“…At the same time, PAL is a sensitive indicator of stressful conditions and is commonly considered as a biochemical marker indicating the synthesis of both structural and protective compounds [32]. The study performed by Ortega-García and Peragón [33] showed that cold stress significantly stimulated PAL activity in leaves of the olive trees. Du et al [34] reported that enhanced UV-B radiation significantly increased PAL activity in fresh-cut carrot products.…”

Section: Enhanced Uv-b Effects On Biochemical Traits In Postharvest Fmentioning

confidence: 97%

Biochemical traits and proteomic changes in postharvest flowers of medicinal chrysanthemum exposed to enhanced UV-B radiation

Yao

Chu

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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The response of phenylalanine ammonia‐lyase, polyphenol oxidase and phenols to cold stress in the olive tree (Olea europaea L. cv. Picual)

Cited by 89 publications

References 51 publications

The physiological and biochemical responses of eastern purple coneflower to freezing stress

The physiological and biochemical responses of eastern purple coneflower to freezing stress

Olive (Olea europaea L.) freezing tolerance related to antioxidant enzymes activity during cold acclimation and non acclimation

Biochemical traits and proteomic changes in postharvest flowers of medicinal chrysanthemum exposed to enhanced UV-B radiation

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