The Response of Lymphocytes to Antigen Challenge in Vitro

Elves, Michael W.; Roath, S.; Israëls, M. C. G.

doi:10.1016/s0140-6736(63)91508-3

Cited by 204 publications

(33 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cells of similar appearance are observed in the regional lymph nodes after in vivo immunization (32). Changes similar to those that occur on PHA stimulation are observed on stimulating lymphocytes with antigens to which a person is known to have been sensitized previously (33,34) and in mixed leukocyte reactions where histocompatibility antigens appear to be the stimulating factors (14). PHA-treated lymphocytes have been shOwn to synthesize immunoglobulins (35)(36)(37), specific antibodies (36,37), and interferon (38).…”

Section: Resultsmentioning

confidence: 78%

DNA polymerase activity as an index of lymphocyte stimulation: studies in Down's syndrome

Ss¹,

Bs²,

Bj³

et al. 1970

J. Clin. Invest.

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 78%

DNA polymerase activity as an index of lymphocyte stimulation: studies in Down's syndrome

Ss¹,

Bs²,

Bj³

et al. 1970

J. Clin. Invest.

View full text Add to dashboard Cite

“…Specific antigens will also induce transformation in lymphocytes from sensitized individuals (10,11). These responses are impaired in patients with lymphomatous disorders such as chronic lymphatic leukemia (12)(13)(14) and Hodgkin's disease (15,16).…”

Section: Introductionmentioning

confidence: 99%

Impaired Lymphocyte Transformation and Delayed Hypersensitivity in Sjögren's Syndrome*

Leventhal

Waldorf²,

Talal³

1967

J. Clin. Invest.

111

View full text Add to dashboard Cite

“…A number of in vitro systems, however, have been developed and are widely used for the study of the cellular immune response to nonviral antigens (2,3). Recently, studies from several laboratories suggest that lymphocytes from specifically immunized animals will respond to viral antigens in vitro, as measured by blastogenesis, migration inhibitory factor production, and cytotoxicity (4)(5)(6)(7)(8)(9)(10)(11). There is, however, little if any quantitative information on the preparation and nature of the viral antigens, the time of appearance and magnitude of the immune response, and the specificity of the reaction.…”

mentioning

confidence: 99%

In Vitro Stimulation of Sensitized Lymphocytes by Herpes Simplex Virus and Vaccinia Virus

Rosenberg

Farber

Notkins

1972

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Splenic lymphocytes from rabbits immunized with herpes simplex virus (HSV) were incubated in vitro with ultraviolet light-inactivated HSV, and the degree of lymphocyte transformation was determined by measurement of the incorporation of [3Hjthymidine into acid-insoluble material. Lymphocytes from immunized rabbits were stimulated as much as 30-fold, whereas lymphocytes from control rabbits were unaffected. Lymphocyte sensitization occurred within 3 days after immunization; sensitized lymphocytes could still be detected 120 days after immunization. The antigenicity of the ultraviolet light-inactivated crude virus pool was found to reside primarily in the virion. Infectious virus, in comparison with inactivated virus, produced less lymphocyte stimulation. Studies on the interaction of the humoral and cellular immune responses showed that incubation of anti-HSV antibody with HSV antigens did not reduce the capacity of the viral antigens to stimulate sensitized lymphocytes. Other experiments revealed that lymphocytes from both the spleen and peripheral blood of animals immunized with vaccinia virus could be stimulated by vaccinia, but not by HSV. Conversely, lymphocytes from animals immunized with HSV could not be stimulated by vaccinia. The transformation of sensitized lymphocytes by viral antigens appears to be a simple, highly specific, and quantitative in vitro technique for the study of the cellular immune response to viral infections.Cell-mediated immunity is thought to be an important factor in the defense against certain viral infections (1), but its precise role has been difficult to assess because of the lack of a simple in vitro assay. A number of in vitro systems, however, have been developed and are widely used for the study of the cellular immune response to nonviral antigens (2, 3). Recently, studies from several laboratories suggest that lymphocytes from specifically immunized animals will respond to viral antigens in vitro, as measured by blastogenesis, migration inhibitory factor production, and cytotoxicity (4-11). There is, however, little if any quantitative information on the preparation and nature of the viral antigens, the time of appearance and magnitude of the immune response, and the specificity of the reaction. The present report on virusinduced lymphocyte stimulation describes a simple and quantitative in vitro system for the study of the cellular immune response to viral antigens, and provides some information on the factors and conditions involved in viruslymphocyte interaction. were grown and assayed in primary rabbit kidney cells (12, 13). The stock pools of crude HSV and vaccinia contained 7.5 X 107 plaque-forming units (PFU)/ml and 4.0 X 106 PFU/ml, respectively. Partially purified virus was prepared by centrifuging the stock virus pools at 78,500 X g for 60 min in a Beckman model L ultracentrifuge. The supernatant fluid was used as the source of soluble viral antigens; the viral pellet was suspended in phosphate-buffered saline (PBS, 0.02 M, pH 7.2), centrifuged at 78,50...

show abstract

The Response of Lymphocytes to Antigen Challenge in Vitro

Cited by 204 publications

References 6 publications

DNA polymerase activity as an index of lymphocyte stimulation: studies in Down's syndrome

DNA polymerase activity as an index of lymphocyte stimulation: studies in Down's syndrome

Impaired Lymphocyte Transformation and Delayed Hypersensitivity in Sjögren's Syndrome*

In Vitro Stimulation of Sensitized Lymphocytes by Herpes Simplex Virus and Vaccinia Virus

Contact Info

Product

Resources

About