The relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes. I. Surface immunoglobulin isotypes on primed B cells in the spleen.

Abstract

We investigated the ability of IgM-, IgD-, and IgG-bearing cells from the spleens of (BALB/c x C57BL/Ka)F1 mice primed to dinitrophenyl-bovine serum albumin (DNP-BSA) to restore the adoptive secondary anti-BSA and anti-DNP antibody responses. A rabbit anti-mouse IgD antiserum was prepared and the specificity documented by radioimmunoprecipitation, and cell surface staining. Purified populations of IgM-, IgD-, and IgG-bearing cells were prepared by immunofluorescent staining with isotype-specific reagents, and sorting on the fluorescence activated cell sorter. Bright or dull cells were transferred to irradiated syngeneic recipients which were challenged with DNP-BSA in saline. Unfractionated spleen cells restored an adoptive secondary serum antibody response which was all IgG (2-mercaptoethanol resistant). Purified IgM- or IgD-bearing cells restored both the secondary IgM and IgG antibody response. IgG-bearing cells restored only the IgG response. In addition, the IgG-bearing cells appear to suppress the adoptive secondary IgM response, since depletion of IgG-bearing cells from transferred spleen cells results in a marked increase in the adoptive IgM response.

Maintenance of long-term immunological memory by Ig+CD45R+ non-plasma B cells following mucosal immunizations

“…This, in turn, was a confirmation of previous findings in which, serum responses to another hapten, DNP, was also dominated by IgM following oral immunizations with DNP-KLH plus CT adjuvant [13]. Early studies also suggested a strong role of IgM in the generation or maintenance of memory following systemic immunizations [15][16][17]. In general, relatively little information is available on the isotype distribution of serum Ig or Ig-secreting cells in various tissues in long-term (over 3 months) memory studies.…”

Blocking of primary in vitro antibody responses to thymus-independent and thymus-dependent antigens with antiserum specific for IgM or IgD.

“…Rabbit anti-6 was judged to be monospecific by published criteria (18), including: (i) a single peak on sodium dodecyl sulfate/polyacrylamide gel electrophoresis after reaction with a lysate of iodinated splenocytes; (ii) immunofluorescent staining of the predicted numbers of cells from various lymphoid tissues; (iii) inability to stain splenocytes after their treatment with either anti-Ig or allotypic (19) anti-6; and (iv) independent capping of surface molecules on splenocytes with anti-6 and anti-A. Chromatographically purified fractions of normal rabbit serum (NRS), anti-,q, anti-Ig, or a 30% (NH4)2SO4 saturation precipitate of anti-6 was used for in vitro cell culture.…”

Structure and Biological Function of Human IgD

“…With the background knowledge of IgD being involved in B lymphocyte differentia tion events [15] and a precursor of IgG cells in the mouse [24] we designed several ex periments to measure immunoglobulin syn thesis by the lymphocytes of PR patients. As shown in table IV the level of serum IgM, IgG, IgD, IgA, IgE in PR during the acute and convalescent stages of the disease are similar to that of normal individuals with a normal frequency of IgD and/or IgM-bearing cells.…”