The relationship between Bag-1 and gastric cancer clinicopathologic characteristic

Yang, Jiyuan; Cai, Zhiqiang; Du, Wei; Li, Junchuan; Chen, Tingxuan; Xu, Yunhua

doi:10.1007/s10330-011-0850-3

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…A total of 499 KIRC samples with BAG1 expression data and patient's clinical pathological parameters from TCGA were analyzed. Unlike some previous studies that have suggested BAG1 may be associated with a poor clinical features [16,17], we found that its expression as a continuous variable showed a significant inverse correlation with the clinical characteristics in KIRC, such as stage (stage I vs. stage IV p < 0.001), tumor size (p < 0.001), Sex (p < 0.001), grade (p < 0.001), serum hemoglobin level (p = 0.018), cancer status (p < 0.01), lymph node metastasis (p = 0.04) and distant metastasis (p < 0.001) ( Fig. 2a-i).…”

Section: Association With Bag1 Expression and Pathologic Variablesmentioning

confidence: 75%

Expression of BAG1 is associated with prognosis in kidney renal clear cell carcinoma based on bioinformatics

Liu

He³

et al. 2021

BMC Cancer

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Background BCL2 associated Athano-Gene 1 (BAG1) has been described to be involved in the development and progression of cancer. But the role of BAG1 in kidney renal clear cell carcinoma (KIRC) has remained largely unknown. Methods We performed bioinformatic analysis of data from TCGA and GEO dataset. The role of BAG1 in KIRC was explored by Logistic and Cox regression model. The molecular mechanisms of BAG1 was revealed by GSEA. Results The current study found that the KIRC tumor samples have a low level of BAG1 mRNA expression compared to the matched normal tissues based on TCGA data and GEO databases. Low expression of BAG1 in KIRC was significantly associated with Sex, clinical pathological stage, tumor-node-metastasis (TNM) stage, hemoglobin levels, cancer status and history of neoadjuvant treatment. Kaplan-Meier survival analysis indicated that KIRC patients with BAG1 high expression have a longer survival time than those with BAG1 low expression (p < 0.000). Cox regression analysis showed that BAG1 remained independently associated with overall survival, with a hazard ratio (HR) of 1.75(CI:1.05–2.90; p = 0.029). GSEA indicated that the signaling pathways including fatty acid metabolism and oxidative phosphorylation were differentially enriched in high BAG1 expression phenotype. Conclusions These findings suggested that BAG1 expression may act as a potential favorable prognostic marker and challenging therapeutic target.

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Section: Association With Bag1 Expression and Pathologic Variablesmentioning

confidence: 75%

Expression of BAG1 is associated with prognosis in kidney renal clear cell carcinoma based on bioinformatics

Liu

He³

et al. 2021

BMC Cancer

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Antiapoptotic gene BAG-1 vector structure of RNA interference and endogenous targeted screening in colon cancer cell lines

et al. 2013

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The purposes of the present work were to construct the shRNA plasmids for BAG-1 gene of human and test the expression of mRNA and protein of BAG-1. Recombinant plasmids containing green fluorescent protein reporter genes are constructed using gene cloning methods. The shRNA plasmids for the BAG-1 gene are constructed by RNA interference technology. We applied fluorescent plasmid-transfected target cells in the cell transfection experiments and monitored the transfection rate of plasmids by observing the fluorescence amount. We transfected three synthesized shRNA in target screening cell and adopted RT-PCR and Western test to identify the difference of target gene transfection and translation level in cells. The specific shRNA plasmid for the BAG-1 gene was successfully recombined, and stably transfected colon cancer Lo Vo cell lines were obtained. The results present that the constructed shRNA plasmids significantly inhibited the expression of mRNA and protein of Lo Vo cell BAG-1, and can maintain the effect for a long term. pGPH1/GFP/Neo-BAG-1-homo-825 was screened as the optimum sequence of interference so as to lay a solid foundation to explore into the research on the BAG-1 gene and the biological behavior of colon cancer cells. It showed the remarkable advantage of RNAi in the generation of posttranscriptional gene silencing.

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The relationship between Bag-1 and gastric cancer clinicopathologic characteristic

Cited by 2 publications

References 8 publications

Expression of BAG1 is associated with prognosis in kidney renal clear cell carcinoma based on bioinformatics

Expression of BAG1 is associated with prognosis in kidney renal clear cell carcinoma based on bioinformatics

Antiapoptotic gene BAG-1 vector structure of RNA interference and endogenous targeted screening in colon cancer cell lines

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