The Regulators of VEGF Expression in Mouse Ovaries

Shin, So Young; Lee, Ho Jung; Ko, Duck Sung; Lee, Hoi Chang; Park, Won Il

doi:10.3349/ymj.2005.46.5.679

Cited by 15 publications

(17 citation statements)

References 29 publications

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“…In the ovary, oxygen is provided to the follicle by blood vessels around it. VEGF is known to stimulate neovascularisation in response to an FSH stimulus in mouse (Shin et al 2005) and rat follicles (Kuo et al 2011). Furthermore, in primate follicles, VEGF inhibition has been shown to upregulate hypoxia-inducible factor 1a (HIF1a; Duncan et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes

Bunel¹,

Nivet²,

Blondin³

et al. 2014

Reprod. Fertil. Dev.

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The final days before ovulation impact significantly on follicular function and oocyte quality. This study investigated the cumulus cell (CC) transcriptomic changes during the oocyte developmental competence acquisition period. Six dairy cows were used for 24 oocyte collections and received FSH twice daily over 3 days, followed by FSH withdrawal for 20, 44, 68 and 92 h in four different oestrous cycles for each of the six cows. Half of the cumulus-oocyte complexes were subjected to in vitro maturation, fertilisation and culture to assess blastocyst rate. The other half of the CC underwent microarray analysis (n=3 cows, 12 oocyte collections) and qRT-PCR (n=3 other cows, 12 oocyte collections). According to blastocyst rates, 20 h of FSH withdrawal led to under-differentiated follicles (49%), 44 and 68 h to the most competent follicles (71% and 61%) and 92 h to over-differentiated ones (51%). Ten genes, from the gene lists corresponding to the three different follicular states, were subjected to qRT-PCR. Interestingly, CYP11A1 and NSDHL gene expression profiles reflected the blastocyst rate. However most genes were associated with the over-differentiated status: GATM, MAN1A1, VNN1 and NRP1. The early period of FSH withdrawal has a minimal effect on cumulus gene expression, whereas the longest period has a very significant one and indicates the beginning of the atresia process.

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Section: Discussionmentioning

confidence: 99%

Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes

Bunel¹,

Nivet²,

Blondin³

et al. 2014

Reprod. Fertil. Dev.

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“…The treated cells were compared with control cultures that were incubated under identical conditions, but in the absence of TGF-β1 or IGF-1. After a 24 hour treatment, total RNA was isolated from the HCE B-3 cells using TRIzol as the extraction reagent (Gibco-Invitrogen, Carlsbad, CA, USA) 9. Cells were used at passage five for all experiments.…”

Section: Methodsmentioning

confidence: 99%

IGF-1 Counteracts TGF-β-Mediated Enhancement of Fibronectin for in Vitro Human Lens Epithelial Cells

et al. 2007

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PurposeTo determine whether insulin-like growth factor (IGF-1) affects transforming growth factor (TGF-β)-mediated fibronectin accumulation in human lens epithelial cell line (HLE B-3) cells.Materials and MethodsHLE B-3 cells were incubated for 24 hours with TGF-β (10 ng/mL), IGF-1 (10 ng/mL), or both. Expression of the fibronectin gene was determined using a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Fibronectin levels were examined using western blot analysis and immunofluorescence staining.ResultsExpression of the fibronectin gene was not different between the TGF-β/IGF-1 treated group and the TGF-β treated group (p = 0.116). However, western blot analysis demonstrated decreased fibronectin levels in human lens epithelial cells treated with TGF-β and IGF-1 compared to those treated with TGF-β only (p < 0.01). Immunofluorescence staining disclosed inhibition of TGF-β-induced fibronectin in the presence of IGF-1.ConclusionThis study suggests that IGF-1 counteracts TGF-β-mediated fibronectin accumulation in human lens epithelial cells.

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“…It is not only involved in the normal physiological processes of ovarian activity, but also involved in the occurrence and development of many ovulatory disorders [11-13]. Numerous factors have been found to regulate VEGF expression, such as hypoxia-inducible factors, cytokines, growth factors and gonadotropins [1, 11, 14-20]. Although numerous studies have shown that inflammatory cytokines are one of the major inducing factors that regulate the expression of VEGF in non-ovarian cells [21-27], whether inflammatory cytokines are involved in regulating FSH-induced VEGF expression in ovarian follicles are still unknown.…”

Section: Introductionmentioning

confidence: 99%

IL-6 Promotes FSH-Induced VEGF Expression Through JAK/STAT3 Signaling Pathway in Bovine Granulosa Cells

Yang¹,

Wang²,

Wang³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Vascular endothelial growth factor (VEGF) has been demonstrated to play a pivotal role in the regulation of angiogenesis in ovarian follicular development, particularly during the preovulatory period. Although numerous studies have shown that interleukin-6 (IL-6) is one of the major inducing factors that regulate the expression of VEGF in non-ovarian cells, whether it involved in regulating the expression of VEGF in normal ovarian granulosa cells is still unknown. The aim of this study was to elucidate the mechanisms underlying the effect of IL-6 on FSH-induced VEGF expression in bovine granulosa cells derived from large follicles. Methods: VEGF mRNA expression in granulosa cells after IL-6 with/without inhibitors treatment was analyzed by RT-qPCR. Phosphorylation levels of ERK1/2 and STAT3 proteins induced by IL-6 were analyzed by western blotting. The protein levels produced by granulosa cells were detected by ELISA. Results: High concentration of IL-6 (10ng/ml) can significantly up-regulate FSH-induced VEGF gene and protein expression levels in granulosa cells, and also promote the VEGF upstream regulators HIF-1α and COX2 mRNA expression. VEGF expression levels were significantly decreased after specifically blocking HIF-1α and COX2 by using inhibitors. The up-regulation effect of IL-6 on FSH-induced VEGF expression in granulosa cells mainly through activating the JAK/STAT3 signaling pathway, which can be impaired by JAK inhibitors. Conclusion: IL-6 can promote FSH-induced VEGF expression in granulosa cells, which is mainly achieved by increasing the expression of HIF-1α and COX2.This promoting effect is mediated by activating the JAK/STAT3 pathway. Moreover, there may be a synergistic relationship between FSH and IL-6 in the regulation of VEGF expression.

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The Regulators of VEGF Expression in Mouse Ovaries

Cited by 15 publications

References 29 publications

Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes

Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes

IGF-1 Counteracts TGF-β-Mediated Enhancement of Fibronectin for in Vitro Human Lens Epithelial Cells

IL-6 Promotes FSH-Induced VEGF Expression Through JAK/STAT3 Signaling Pathway in Bovine Granulosa Cells

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