The regulation of leptin gene expression in the C6 glioblastoma cell line

Morash, Barbara; Johnstone, Jennie; Leopold, Cindee; Li, Audrey; Murphy, Paul R.; Ur, Ehud; Wilkinson, Michael

doi:10.1016/s0303-7207(00)00259-8

Cited by 46 publications

(39 citation statements)

References 46 publications

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“…Moreover, cAMP increased leptin promoter activity and leptin mRNA transcription . The stimulatory cAMP effect on the leptin expression was also reported in several cells such as glioma cells, pituitary tumor cells (Morash et al 2000, Li et al 2001, breast cancer cells (O'Neil et al 2001), gastric cells (Goiot et al 2005) and placental chorionic tissue (Yura et al 1998).…”

Section: Signal Transduction Pathways Mediating Leptin Inductionmentioning

confidence: 63%

Involvement of leptin in the molecular physiology of the placenta

Schanton¹,

Maymó²,

Pérez-Pérez³

et al. 2018

Reproduction

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Leptin is a homeostatic regulator in the placenta where it promotes proliferation, protein synthesis and the expression of tolerogenic maternal response molecules such as HLA-G. Leptin also exerts an anti-apoptotic action in placenta controlling the expression of p53 master cell cycle regulator under different stress conditions. On the other hand, leptin is an integrative target of different placental stimuli

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Section: Signal Transduction Pathways Mediating Leptin Inductionmentioning

confidence: 63%

Involvement of leptin in the molecular physiology of the placenta

Schanton¹,

Maymó²,

Pérez-Pérez³

et al. 2018

Reproduction

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“…MMP-13 expression is also reported to be induced via NF-κB activation in chondrocytes (Liacini et al 2003;Ohno et al 2006). Since Morash et al (2000) reported that C6 glioma cells do not express long form of leptin receptor (ObRl), all the signal transduction observed in this study is due to the activation of short form of leptin receptor (ObRt). These results indicate that other than the role in leptin transporter, short form of leptin receptor also plays a notable role in mediating signal transduction (Bjorbaek et al 1997;Murakami et al 1997).…”

Section: Discussionmentioning

confidence: 72%

“…The expression of both leptin and leptin receptors detected in C6 glioma cells suggests a possible autocrine role for leptin homeostasis (Morash et al 2000). In this study, we hypothesized that leptin plays an important role in the functional regulation of tumor.…”

Section: Introductionmentioning

confidence: 89%

“…It is also reported that factors expressed during the developmental stage of the CNS exhibits an overexpression pattern by neoplastic cells in mature brain (Merrill and Oldfield 2005;Plata-Salaman 1991;Trojan et al 2007). Since Morash et al (2000) demonstrate that C6 glioma cells do not express long form of leptin receptor, we thus compared the expression of leptin and short form of leptin receptor between cancer and normal cell line of astrocytes. It was found that as compared with non-malignant astrocytes RBA1 and NRA, malignant C6 glioma cells expressed more leptin and leptin receptor in both mRNA and protein levels (Fig.…”

Section: Leptin Increases the Migration And Invasion Of C6 Glioma Cellsmentioning

confidence: 99%

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Leptin induces migration and invasion of glioma cells through MMP‐13 production

Yeh

Lee

et al. 2008

Glia

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Leptin, the product of the obses gene, plays an important role in the regulation of body weight by coordinating metabolism, feeding behavior, energy balance and neuroendocrine responses. However, central regulation of leptin gene expression is different from that in the adipocytes. In addition, leptin has been found in many tumor cell lines and has been shown to have mitogenic and angiogenic activity in a number of cell types. Glioma is the most common primary adult brain tumor with poor prognosis due to the spreading of tumor cell to the other regions of brain easily. Here we found that malignant C6 glioma cells expressed more leptin and leptin receptors than non-malignant astrocytes. Furthermore, it was found that exogenous application of leptin enhanced the migration and invasion of C6 glioma cells. In addition, we found that the expression of matrix metalloproteinase-13 (MMP-13) but not of MMP-2 and MMP-9 was increased in response to leptin stimulation. The leptin-induced increase of cell migration and invasion was antagonized by MMP-13 neutralizing antibody or silencing MMP-13. The up-regulation of MMP-13 induced by leptin was mainly through p38 MAP kinase and NF-κB pathway. In addition, migration-prone sublines demonstrate that cells with increasing migration ability had more expression of MMP-13 and leptin. Taken together, these results indicate that leptin enhanced migration and invasion of C6 glioma cells through the increase of 2

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“…May 2015 | Volume 3 | Issue 5 | Page 304 ment (Morash et al, 2000). Leptin also seemed to be an important factor for growth of many cell types as pancreatic cells (Tanabe et al, 1997), white blood cells (Lord et al, 1998), and epithelial cells of trachea and squamous cells of lungs (Tsuchiya et al, 1999).…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Role of Leptin in Growth, Reproduction and Milk Production in Farm Animals: A Review

Saleem¹

2015

Adv. Anim. Vet. Sci.

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| Livestock species of agri-based countries, like Pakistan, need special attention for the improvement and sustainability of their productive traits because the economy and huge population is largely dependent on livestock. Therefore it is necessary to adopt some strategies to get more adaptive and productive animals. Leptin, a hormone produced mostly by white adipose tissues of body, has many roles including body energy balance, tissue growth, body composition, reproduction and immunity. Leptin helps in reducing feed intake of animal through feed-back mechanism by informing CNS about body energy and fat reserves. The gene responsible for leptin production has many forms which are associated with leptin production, its level in blood and working. The physiological role of leptin and association of different forms of leptin (LEP) gene with multiple economic traits has made it a potential candidate to be studied and improve animals through proper breeding and management strategies. This current review article discusses the role of leptin in various economic traits of farm animals.

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The regulation of leptin gene expression in the C6 glioblastoma cell line

Cited by 46 publications

References 46 publications

Involvement of leptin in the molecular physiology of the placenta

Involvement of leptin in the molecular physiology of the placenta

Leptin induces migration and invasion of glioma cells through MMP‐13 production

Role of Leptin in Growth, Reproduction and Milk Production in Farm Animals: A Review

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