The Receptor Kinase CORYNE ofArabidopsisTransmits the Stem Cell–Limiting Signal CLAVATA3 Independently of CLAVATA1

Abstract

Stem cells in shoot and floral meristems of Arabidopsis thaliana secrete the signaling peptide CLAVATA3 (CLV3) that restricts stem cell proliferation and promotes differentiation. The CLV3 signaling pathway is proposed to comprise the receptor kinase CLV1 and the receptor-like protein CLV2. We show here that the novel receptor kinase CORYNE (CRN) and CLV2 act together, and in parallel with CLV1, to perceive the CLV3 signal. Mutations in CRN cause stem cell proliferation, similar to clv1, clv2, and clv3 mutants. CRN has additional functions during plant development, including floral organ development, that are shared with CLV2. The CRN protein lacks a distinct extracellular domain, and we propose that CRN and CLV2 interact via their transmembrane domains to establish a functional receptor.

Plant GSK3 proteins regulate xylem cell differentiation downstream of TDIF–TDR signalling

“…1a). Detailed Y2H assays showed that the full length BIN2 protein interacts with TDRKD, but not with the kinase domain of CRN, another receptor-like protein functioning in CLE signalling 9,20,21 (Fig. 1a, and Supplementary Fig.…”

“…The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGIONrelated (CLE) family of signalling peptides plays crucial roles in the regulation of stem cell maintenance in the meristems 3 . In the shoot apical meristem of Arabidopsis thaliana, CLV3 peptide secreted from stem cells restricts the expression domain of the WUSCHEL (WUS) homeobox transcription factor gene to the organizing centre via three receptor pathways, CLV1, CLV2-CORYNE (CRN) and RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2) [4][5][6][7][8][9][10] . CLV3-WUS signalling makes a negative feedback loop to maintain the homoeostasis of stem cell population in the shoot apical meristem [11][12][13] .…”

RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor 1 in Arabidopsis thaliana

“…For example, CLV3 and CLE40 are responsible for shoot and root apical meristem maintenance, respectively [9,11,12]. CLV3 is perceived by three different receptors or putative receptors, CLAVA-TA1 (CLV1), CLAVATA 2 (CLV2)-CORYNE (CRN), and RPK2/TOAD2, to limit stem cell size by restricting the expression of a homeodomain transcription factor, WUSCHEL (WUS), via a negative feedback loop [13][14][15][16][17][18]. On the other hand, CLE40 is perceived by its putative receptor complex containing two different RLKs, ARA-BIDOPSIS CRIKLY4 (ACR4) and CLV1, to inhibit the expression of a root version of homeodomain transcription factor, WOX5 [19,20].…”

Mystery in genetics: PUB4 gives a clue to the complex mechanism of CLV signaling pathway in the shoot apical meristem

“…7 Genetic approach has demonstrated that these 3 receptor complexes function downstream of CLV3 basically, independently of each other, however, it remains unclear whether they are functionally redundant or each has distinct functions. [6][7][8]15 Previously, we have shown that a synthetic CLV3 peptide, MCLV3 could be a useful tool for investigating downstream components of the CLV signaling pathway. 7,9 Here we report the defects of SAM in a recently identified MCLV3 resistant mutant, pub4, 10 and discuss the possible relationship of its causal gene with the CLV signaling pathway.…”

“…[1][2][3] CLV2, a receptor-like protein, associates with CORYNE (CRN)/SUPPRESSOR OF LLP1 2 (SOL2), a membrane associated protein pseudo-kinase with a short extracellular domain. [4][5][6] RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2) is another receptor-like kinase that is essential for CLV3 signaling. 7 Genetic approach has demonstrated that these 3 receptor complexes function downstream of CLV3 basically, independently of each other, however, it remains unclear whether they are functionally redundant or each has distinct functions.…”