The Recent Development in Technologies for Attaining Doubled Haploid Plants In Vivo

Abstract

Haploid plants with a doubled set of chromosomes (doubled haploid (DH)) significantly speed up the selection process by the fixation of genetic traits in each locus in the homozygous state within one generation. Doubled haploids are mainly attained by the formation of plants from the cultured gametophytic (haploid) tissues and cells in vitro, or by targeted reduction in the parent chromosome during intra- or interspecific hybridization. Since then, DH has become one of the most powerful tools to support various basic research studies, as well as applied research. This review is focused on the recent development of the production of doubled haploids in vivo and their fundamental bases. The various mechanisms and approaches responsible for the formation of haploids in vivo are discussed, particularly the induction of parthenogenesis by BBM-like proteins, the long constructed Salmon system of wheat, the usage of patatin-like phospholipases MTL/PLA1/NLD, the IG1 system, uniparental genome elimination during interspecific hybridization, and the perspective technology of centromeric histone 3 (CENH3) modification.

Genetic resources and breeding of maize for Striga resistance: a review

“…The DH plants are derivatives of haploid plants with a doubled set of the chromosome. The DH method significantly speeds up the selection process by fixing genes in the homozygous state ( Zargar et al., 2022 ). The DH method has several benefits, including creating lines with 100% homozygosity.…”

Ameliorating Spring Wheat Productivity in Drought Conditions: Identification of Correlations of Rht (-b1a, -b1b, d1a, d1b) Semi-dwarfism Genes and Coarse-Grained TaGW8-b1a, TaGS 5–3a

“…However, the GS, the spike's structure, the PH, and the signs associated with the flag leaf can also affect the yield by affecting the intensity of photosynthesis, grain filling, and movement of dry matter (Zhou et al, 2007). These traits have a higher heritability than productivity and are easier to select on small plots at the early stages of the breeding process (Faji et al, 2019;Zargar et al, 2022).…”

Technology for Production of Wheat Doubled Haploid via Maize Pollen Induction—Updated Review

“…In vitro culture includes androgenesis (anther or microspore culture) and gynogenesis (bud, ovary, or unfertilized ovule culture). In vivo methods include induction of parthenogenesis by inactive pollen pollination (ionizing radiation, chemical agents, and high temperature) and intra-or interspecific hybridization, also known as the chromosome elimination method [15]. The effectiveness of different methods depends on plant species [13].…”