The rapid diagnosis of infectious mononucleosis using an ELISA that detects IgM antibody to a peptide component of epstein-barr virus nuclear antigen

Levin, Myron J.; Weinstein, Melvin P.; Sumaya, Ciro V.; Gooch, M. E.; Osband, Michael E.; Rhodes, Gary; Ackley, Alexander; Wren, C G; Podgore, John K.

doi:10.1016/0732-8893(91)90018-b

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…To overcome the disadvantage of past technologies, hyphenated technique of ELISA was considered to develop due to peptide ELISA has also been used in the clinical diagnosis of severe diseases, as evidenced by numerous reports [32][33][34]. Many companies, such as Sigma, Promega, and Perkin Elmer Life Science, have developed ELISA kits for peptides.…”

Section: Introductionmentioning

confidence: 99%

Epitopic Peptides Identified by LC–ELISA and LC–MS

Zhou

Wang

2011

Chromatographia

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A new strategy of a liquid chromatography (LC)-enzyme linked immunosorbent assay (ELISA) combined with liquid chromatography-mass spectrometry (LC-MS) was developed to identify the epitopic peptides for the antigen in Streptococcus suis. Metallendopeptidase, a potential antigen for S. suis, was digested by trypsin. The tryptic solution was separated by LC under optimal condition and collected into tubes. After it was dried using a cooling vacuum, the immunogenecity of peptides in each tube was investigated by ELISA against its corresponding antibody. All peptides in each tube with positive signals based on ELISA results were identified by LC-MS. Accordingly, six putative peptides of metallendopeptidase were found and synthesized, all of which possessed high antigenicity recognized by the antibody.

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Section: Introductionmentioning

confidence: 99%

Epitopic Peptides Identified by LC–ELISA and LC–MS

Zhou

Wang

2011

Chromatographia

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“…An ELISA kit that detects IgG and IgM antibodies to a specific peptide sequence (p62) of EBNA-1 that appears early in acute EBV infections (MONOLERT; Meridian Diagnostics) was recently developed as a rapid diagnostic test for infectious mononucleosis. However, several investigators have found the accuracy of the MONOLERT to be unsatisfactory and the kit unsuitable for the diagnosis of infectious mononucleosis (7,8,10).…”

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confidence: 99%