The qualitative and quantitative analysis of insect hemolymph sugars by high performance thin-layer chromatography

The physiology of host feeding in parasitic wasps: implications for survival

“…Trehalose is known to be the main sugar in insects’ haemolymph, where it is the key energy storage molecule (Florkin & Jeuniaux 1964; Chapman 1998). The trehalose concentration we obtained (10·6 µg µl −1 ) is also very consistent with that obtained in other Hymenoptera and in Coleoptera ( Apis mellifera 10·4 µg µl −1 , Tenebrio molitor 15·5 µg µl −1 , Periplaneta americana 15·3 µg µl −1 , Heliothis zea 10·4 µg µl −1 ; see Fell 1990 and Leta, Gilbert & Morse 1996). The presence of sucrose seems less common but has also been reported in butterfly larvae (Bounias 1983).…”

The physiology of host feeding in parasitic wasps: implications for survival

“…Trehalose is known to be the main sugar in insects’ haemolymph, where it is the key energy storage molecule (Florkin & Jeuniaux 1964; Chapman 1998). The trehalose concentration we obtained (10·6 µg µl −1 ) is also very consistent with that obtained in other Hymenoptera and in Coleoptera ( Apis mellifera 10·4 µg µl −1 , Tenebrio molitor 15·5 µg µl −1 , Periplaneta americana 15·3 µg µl −1 , Heliothis zea 10·4 µg µl −1 ; see Fell 1990 and Leta, Gilbert & Morse 1996). The presence of sucrose seems less common but has also been reported in butterfly larvae (Bounias 1983).…”

Effect of metacestodes of Hymenolepis diminuta on storage and circulating carbohydrates in the intermediate host, Tenebrio molitor

“…These trehalose values were considerably lower than those recorded in this study. However, our values for trehalose are in accord with those of Fell (1990). Using high performance thin layer chromatography, Fell was unable to detect haemolymph glucose.…”

Systemic glucose levels are modulated by specific wavelengths in the solar light spectrum that shift mitochondrial metabolism

“…Thus, glucose challenge responses represent those at rest. The large capacity for sugar concentration within bee homeostasis [ 26 ], and the rest state of the bees fit with the long duration of elevated glucose levels measured ( Fig 1A ). The two-hour delay until peak glucose level represents the initial storage capacity of the crop with gradual release into the haemolymph [ 20 ].…”

The physiology of host feeding in parasitic wasps: implications for survival

“…Trehalose is known to be the main sugar in insects’ haemolymph, where it is the key energy storage molecule (Florkin & Jeuniaux 1964; Chapman 1998). The trehalose concentration we obtained (10·6 µg µl −1 ) is also very consistent with that obtained in other Hymenoptera and in Coleoptera ( Apis mellifera 10·4 µg µl −1 , Tenebrio molitor 15·5 µg µl −1 , Periplaneta americana 15·3 µg µl −1 , Heliothis zea 10·4 µg µl −1 ; see Fell 1990 and Leta, Gilbert & Morse 1996). The presence of sucrose seems less common but has also been reported in butterfly larvae (Bounias 1983).…”

Effect of metacestodes of Hymenolepis diminuta on storage and circulating carbohydrates in the intermediate host, Tenebrio molitor

“…These trehalose values were considerably lower than those recorded in this study. However, our values for trehalose are in accord with those of Fell (1990). Using high performance thin layer chromatography, Fell was unable to detect haemolymph glucose.…”

Systemic glucose levels are modulated by specific wavelengths in the solar light spectrum that shift mitochondrial metabolism

“…Thus, glucose challenge responses represent those at rest. The large capacity for sugar concentration within bee homeostasis [ 26 ], and the rest state of the bees fit with the long duration of elevated glucose levels measured ( Fig 1A ). The two-hour delay until peak glucose level represents the initial storage capacity of the crop with gradual release into the haemolymph [ 20 ].…”

The physiology of host feeding in parasitic wasps: implications for survival

“…Trehalose is known to be the main sugar in insects’ haemolymph, where it is the key energy storage molecule (Florkin & Jeuniaux 1964; Chapman 1998). The trehalose concentration we obtained (10·6 µg µl −1 ) is also very consistent with that obtained in other Hymenoptera and in Coleoptera ( Apis mellifera 10·4 µg µl −1 , Tenebrio molitor 15·5 µg µl −1 , Periplaneta americana 15·3 µg µl −1 , Heliothis zea 10·4 µg µl −1 ; see Fell 1990 and Leta, Gilbert & Morse 1996). The presence of sucrose seems less common but has also been reported in butterfly larvae (Bounias 1983).…”

Effect of metacestodes of Hymenolepis diminuta on storage and circulating carbohydrates in the intermediate host, Tenebrio molitor

“…These trehalose values were considerably lower than those recorded in this study. However, our values for trehalose are in accord with those of Fell (1990). Using high performance thin layer chromatography, Fell was unable to detect haemolymph glucose.…”

Systemic glucose levels are modulated by specific wavelengths in the solar light spectrum that shift mitochondrial metabolism

“…Thus, glucose challenge responses represent those at rest. The large capacity for sugar concentration within bee homeostasis [ 26 ], and the rest state of the bees fit with the long duration of elevated glucose levels measured ( Fig 1A ). The two-hour delay until peak glucose level represents the initial storage capacity of the crop with gradual release into the haemolymph [ 20 ].…”