The Proteome of Hand Eczema Assessed by Tape Stripping

Sølberg, Julie; Quaade, Anna Sophie; Drici, Lylia; Sulek, Karolina; Ulrich, Nina H.; Løvendorf, Marianne B.; Thyssen, Jacob P.; Mann, Matthias; Dyring‐Andersen, Beatrice; Johansen, Jeanne Duus

doi:10.1016/j.jid.2022.12.024

Cited by 9 publications

(1 citation statement)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on classic two-dimensional gel electrophoresis 57 and immunohistochemistry studies 35 which failed to detect vimentin in the epidermis, it has typically been regarded as unimportant in that tissue. Yet these methods are relatively insensitive; more recently published proteomics datasets do detect vimentin in epidermis 58–60 , isolated primary keratinocytes 59 , and another keratinocyte cell line 61 , albeit at lower levels than keratin isoforms (Figure S5B). The role of epidermal vimentin in wound healing has also been unclear.…”

Section: Resultsmentioning

confidence: 99%

Keratin isoform shifts modulate motility signals during wound healing

Nanes

Bhatt

Azarova

et al. 2023

Preprint

View full text Add to dashboard Cite

Keratin intermediate filaments form strong mechanical scaffolds that confer structural stability to epithelial tissues, but the reason this function requires a protein family with fifty-four isoforms is not understood. During skin wound healing, a shift in keratin isoform expression alters the composition of keratin filaments. How this change modulates cellular function to support epidermal remodeling remains unclear. We report an unexpected effect of keratin isoform variation on kinase signal transduction. Increased expression of wound-associated keratin 6A, but not of steady-state keratin 5, potentiated keratinocyte migration and wound closure without compromising epidermal stability by activating myosin motors. This pathway depended on isoform-specific interaction between intrinsically disordered keratin head domains and non-filamentous vimentin shuttling myosin-activating kinases. These results substantially expand the functional repertoire of intermediate filaments from their canonical role as mechanical scaffolds to include roles as signaling scaffolds that spatiotemporally organize signal transduction cascades depending on isoform composition.

show abstract