The protein Slr1143 is an active diguanylate cyclase in Synechocystis sp. PCC 6803 and interacts with the photoreceptor Cph2

Angerer, Veronika; Schwenk, Philipp; Wallner, Thomas; Kaever, Volkhard; Hiltbrunner, Andreas; Wilde, Annegret

doi:10.1099/mic.0.000475

Cited by 21 publications

(19 citation statements)

References 57 publications

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“…Photoreceptor Cph2 with the GGDEF domain acts as a blue-light triggered c-di-GMP producer and thereby inhibits cell motility in blue light. Furthermore, Cph2 modulates motility by interacting with Cip1 (Slr1143, Cph2interacting protein 1) under red light (Angerer et al, 2017). MCP-CheA-CheY systems include sll0038-sll0043, slr1041-slr1044 and slr0322, slr0073, sll1291-sll1296 three gene clusters (Chung et al, 2001;Yoshihara et al, 2002).…”

Section: Motility Signaling Pathwaymentioning

confidence: 99%

Recent Advances in Biological Functions of Thick Pili in the Cyanobacterium Synechocystis sp. PCC 6803

Chen

Tan

et al. 2020

Front. Plant Sci.

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Cyanobacteria have evolved various strategies to sense and adapt to biotic and abiotic stresses including active movement. Motility in cyanobacteria utilizing the type IV pili (TFP) is useful to cope with changing environmental conditions. The model cyanobacterium Synechocystis sp. PCC 6803 (hereafter named Synechocystis) exhibits motility via TFP called thick pili, and uses it to seek out favorable light/nutrition or escape from unfavorable conditions. Recently, a number of studies on Synechocystis thick pili have been undertaken. Molecular approaches support the role of the pilin in motility, cell adhesion, metal utilization, and natural competence in Synechocystis. This review summarizes the most recent studies on the function of thick pili as well as their formation and regulation in this cyanobacterium.

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Section: Motility Signaling Pathwaymentioning

confidence: 99%

Recent Advances in Biological Functions of Thick Pili in the Cyanobacterium Synechocystis sp. PCC 6803

Chen

Tan

et al. 2020

Front. Plant Sci.

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“… 2013 ; Angerer et al. 2017 ). Interestingly, the difference in the c-di-GMP concentration between green and blue light-grown cells is just 50%, which is in good agreement with in vitro measurements of the light-dependent enzymatic activity of CBCR-GGDEF module of Cph2 (Savakis et al.…”

Section: Light-controlled Motility In Cyanobacteriamentioning

confidence: 99%

“…An interaction of the EAL and GGDEF domains of Cph2 with another c-di-GMP synthesizing enzyme, Slr1143, was recently demonstrated, although this interaction is not light-dependent in vivo (Angerer et al. 2017 ). Nevertheless, Δslr1143 mutant cells show altered motility behavior, moving towards a high-intensity red light source under conditions where the wild type is non-motile (Angerer et al.…”

Section: Light-controlled Motility In Cyanobacteriamentioning

confidence: 99%

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Light-controlled motility in prokaryotes and the problem of directional light perception

Wilde

Mullineaux

2017

FEMS Microbiology Reviews

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The natural light environment is important to many prokaryotes. Most obviously, phototrophic prokaryotes need to acclimate their photosynthetic apparatus to the prevailing light conditions, and such acclimation is frequently complemented by motility to enable cells to relocate in search of more favorable illumination conditions. Non-phototrophic prokaryotes may also seek to avoid light at damaging intensities and wavelengths, and many prokaryotes with diverse lifestyles could potentially exploit light signals as a rich source of information about their surroundings and a cue for acclimation and behavior. Here we discuss our current understanding of the ways in which bacteria can perceive the intensity, wavelength and direction of illumination, and the signal transduction networks that link light perception to the control of motile behavior. We discuss the problems of light perception at the prokaryotic scale, and the challenge of directional light perception in small bacterial cells. We explain the peculiarities and the common features of light-controlled motility systems in prokaryotes as diverse as cyanobacteria, purple photosynthetic bacteria, chemoheterotrophic bacteria and haloarchaea.

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“…All sequenced L. monocytogenes strains contain 3 DGCs and 3 PDEs (5). Elevated c-di-GMP levels in L. monocytogenes strains impaired in invasion were caused either by the expression of a constitutive heterologous DGC, Slr1143 (recently designated Cip1 [11]), or by the deletion of all 3 PDE genes (5). Furthermore, bioinformatics analysis of listerial genomes revealed no homologs of known c-di-GMP-binding receptors/effector proteins, beyond the activator of exopolysaccharide synthesis, PssE (5).…”

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confidence: 99%

CodY-Mediated c-di-GMP-Dependent Inhibition of Mammalian Cell Invasion in Listeria monocytogenes

2018

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Elevated levels of the second messenger c-di-GMP suppress virulence in diverse pathogenic bacteria, yet mechanisms are poorly characterized. In the foodborne pathogen , high c-di-GMP levels inhibit mammalian cell invasion. Here, we show that invasion is impaired because of the decreased expression levels of internalin genes whose products are involved in invasion. We further show that at high c-di-GMP levels, the expression of the entire virulence regulon is suppressed, and so is the expression of the gene encoding the master activator of the virulence regulon. Analysis of mechanisms controlling expression pointed to the transcription factor CodY as a c-di-GMP-sensitive component. In high-c-di-GMP strains, gene expression is decreased, apparently due to the lower activity of CodY, which functions as an activator of transcription. We found that listerial CodY does not bind c-di-GMP and therefore investigated whether c-di-GMP levels affect two known cofactors of listerial CodY, branched-chain amino acids and GTP. Our manipulation of branched-chain amino acid levels did not perturb the c-di-GMP effect; however, our replacement of listerial CodY with the streptococcal CodY homolog, whose activity is GTP independent, abolished the c-di-GMP effect. The results of this study suggest that elevated c-di-GMP levels decrease the activity of the coordinator of metabolism and virulence, CodY, possibly via lower GTP levels, and that decreased CodY activity suppresses virulence by the decreased expression of the PrfA virulence regulon. is a pathogen causing listeriosis, a disease responsible for the highest mortality rate among foodborne diseases. Understanding how the virulence of this pathogen is regulated is important for developing treatments to decrease the frequency of listerial infections in susceptible populations. In this study, we describe the mechanism through which elevated levels of the second messenger c-di-GMP inhibit listerial invasion in mammalian cells. Inhibition is caused by the decreased activity of the transcription factor CodY that coordinates metabolism and virulence.

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The protein Slr1143 is an active diguanylate cyclase in Synechocystis sp. PCC 6803 and interacts with the photoreceptor Cph2

Cited by 21 publications

References 57 publications

Recent Advances in Biological Functions of Thick Pili in the Cyanobacterium Synechocystis sp. PCC 6803

Recent Advances in Biological Functions of Thick Pili in the Cyanobacterium Synechocystis sp. PCC 6803

Light-controlled motility in prokaryotes and the problem of directional light perception

CodY-Mediated c-di-GMP-Dependent Inhibition of Mammalian Cell Invasion in Listeria monocytogenes

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