The Protein Content of the Re-Formed Aqueous Humor in Man

Fluorophotometric and tonometric evaluation of ocular effects following aqueocentesis performed with needles of various sizes in dogs

“…It is logical that a larger needle stimulates more corneal sensory nerves or results in greater corneal tissue trauma, ultimately releasing additional inflammatory mediators, compared with a smaller needle; however, consistent topical anesthesia should have minimized these responses. Although the degree of ocular hypotony does affect blood-aqueous barrier breakdown and causes increased protein content in the reformed aqueous, 44 in the present study the mean IOP immediately after aqueocentesis was not significantly different between treatment groups (Figure 1). The speed of fluid flow into the needle could also be considered as a cause.…”

“…The speed of fluid flow into the needle could also be considered as a cause. A 1940 study 44 revealed that speed of aspiration during aqueous paracentesis in humans had no effect on protein content of the reformed aqueous humor; however, aqueous protein content was measured subjectively via turbidity following addition of sulphosalicylic acid. Quantitative evaluation of aqueous humor protein concentration may reveal that outflow speed does affect blood-aqueous barrier breakdown and therefore may be the cause of differences in our study.…”

Fluorophotometric and tonometric evaluation of ocular effects following aqueocentesis performed with needles of various sizes in dogs

“…It is logical that a larger needle stimulates more corneal sensory nerves or results in greater corneal tissue trauma, ultimately releasing additional inflammatory mediators, compared with a smaller needle; however, consistent topical anesthesia should have minimized these responses. Although the degree of ocular hypotony does affect blood-aqueous barrier breakdown and causes increased protein content in the reformed aqueous, 44 in the present study the mean IOP immediately after aqueocentesis was not significantly different between treatment groups (Figure 1). The speed of fluid flow into the needle could also be considered as a cause.…”

“…The speed of fluid flow into the needle could also be considered as a cause. A 1940 study 44 revealed that speed of aspiration during aqueous paracentesis in humans had no effect on protein content of the reformed aqueous humor; however, aqueous protein content was measured subjectively via turbidity following addition of sulphosalicylic acid. Quantitative evaluation of aqueous humor protein concentration may reveal that outflow speed does affect blood-aqueous barrier breakdown and therefore may be the cause of differences in our study.…”

Laser flaremetric evaluation of experimentally induced blood-aqueous barrier disruption in cats

“…After initial ocular hypotension, paracentesis causes transient ocular hypertension, 6,54 miosis, breakdown of the BAB, [55][56][57] and a subsequent increase in the aqueous humor protein concentration in several species. 6,[58][59][60][61][62][63][64] In our study, mean maximum aqueous humor protein concentration in treated eyes was 434.9 mg/dl 4 hours after paracentesis. At 26 hours, protein concentration decreased to 102.6 mg/dl, which is compatible with results of other studies [65][66][67][68] indicating that paracentesis of the anterior chamber results in a transient breakdown of the BAB.…”

Protein Content of Normal Human Aqueous Humour in Vivo

“…content of aqueous humour is independent of the rate of aspiration, whereas some agents used to produce topical anaesthesia may raise the protein content of aqueous humour (Kronfeld, Lin & Luo 1941), as also do other pharmacological agents in topical use (Seidel 1920, Kadin 1963, Michiels 1964. The anaesthetic used in paracentesis therefore brings a potential source of error in protein studies.…”

“…Any irritation of the eye may, by axon reflexes, produce vasodilation and hence alterations in the chemical composition of the aqueous humour (Adler 1965). Kronfeld, Lin & Luo (1941) considered that, from the point of view of protein content, the instrument by which the bulb was fixed during paracenteslis did not matter, and that the paracentesis in itself did not affect the protein content. But if a failure is immediately followed by a successful paracentesis, the proteins are, however, nearly always increased in the specimen drawn (Kronfeld 1941).…”

Fluorophotometric and tonometric evaluation of ocular effects following aqueocentesis performed with needles of various sizes in dogs

“…It is logical that a larger needle stimulates more corneal sensory nerves or results in greater corneal tissue trauma, ultimately releasing additional inflammatory mediators, compared with a smaller needle; however, consistent topical anesthesia should have minimized these responses. Although the degree of ocular hypotony does affect blood-aqueous barrier breakdown and causes increased protein content in the reformed aqueous, 44 in the present study the mean IOP immediately after aqueocentesis was not significantly different between treatment groups (Figure 1). The speed of fluid flow into the needle could also be considered as a cause.…”

“…The speed of fluid flow into the needle could also be considered as a cause. A 1940 study 44 revealed that speed of aspiration during aqueous paracentesis in humans had no effect on protein content of the reformed aqueous humor; however, aqueous protein content was measured subjectively via turbidity following addition of sulphosalicylic acid. Quantitative evaluation of aqueous humor protein concentration may reveal that outflow speed does affect blood-aqueous barrier breakdown and therefore may be the cause of differences in our study.…”

Laser flaremetric evaluation of experimentally induced blood-aqueous barrier disruption in cats

“…After initial ocular hypotension, paracentesis causes transient ocular hypertension, 6,54 miosis, breakdown of the BAB, [55][56][57] and a subsequent increase in the aqueous humor protein concentration in several species. 6,[58][59][60][61][62][63][64] In our study, mean maximum aqueous humor protein concentration in treated eyes was 434.9 mg/dl 4 hours after paracentesis. At 26 hours, protein concentration decreased to 102.6 mg/dl, which is compatible with results of other studies [65][66][67][68] indicating that paracentesis of the anterior chamber results in a transient breakdown of the BAB.…”

Protein Content of Normal Human Aqueous Humour in Vivo

“…content of aqueous humour is independent of the rate of aspiration, whereas some agents used to produce topical anaesthesia may raise the protein content of aqueous humour (Kronfeld, Lin & Luo 1941), as also do other pharmacological agents in topical use (Seidel 1920, Kadin 1963, Michiels 1964. The anaesthetic used in paracentesis therefore brings a potential source of error in protein studies.…”

“…Any irritation of the eye may, by axon reflexes, produce vasodilation and hence alterations in the chemical composition of the aqueous humour (Adler 1965). Kronfeld, Lin & Luo (1941) considered that, from the point of view of protein content, the instrument by which the bulb was fixed during paracenteslis did not matter, and that the paracentesis in itself did not affect the protein content. But if a failure is immediately followed by a successful paracentesis, the proteins are, however, nearly always increased in the specimen drawn (Kronfeld 1941).…”

Fluorophotometric and tonometric evaluation of ocular effects following aqueocentesis performed with needles of various sizes in dogs

“…It is logical that a larger needle stimulates more corneal sensory nerves or results in greater corneal tissue trauma, ultimately releasing additional inflammatory mediators, compared with a smaller needle; however, consistent topical anesthesia should have minimized these responses. Although the degree of ocular hypotony does affect blood-aqueous barrier breakdown and causes increased protein content in the reformed aqueous, 44 in the present study the mean IOP immediately after aqueocentesis was not significantly different between treatment groups (Figure 1). The speed of fluid flow into the needle could also be considered as a cause.…”

“…The speed of fluid flow into the needle could also be considered as a cause. A 1940 study 44 revealed that speed of aspiration during aqueous paracentesis in humans had no effect on protein content of the reformed aqueous humor; however, aqueous protein content was measured subjectively via turbidity following addition of sulphosalicylic acid. Quantitative evaluation of aqueous humor protein concentration may reveal that outflow speed does affect blood-aqueous barrier breakdown and therefore may be the cause of differences in our study.…”

Laser flaremetric evaluation of experimentally induced blood-aqueous barrier disruption in cats

“…After initial ocular hypotension, paracentesis causes transient ocular hypertension, 6,54 miosis, breakdown of the BAB, [55][56][57] and a subsequent increase in the aqueous humor protein concentration in several species. 6,[58][59][60][61][62][63][64] In our study, mean maximum aqueous humor protein concentration in treated eyes was 434.9 mg/dl 4 hours after paracentesis. At 26 hours, protein concentration decreased to 102.6 mg/dl, which is compatible with results of other studies [65][66][67][68] indicating that paracentesis of the anterior chamber results in a transient breakdown of the BAB.…”

Protein Content of Normal Human Aqueous Humour in Vivo

“…content of aqueous humour is independent of the rate of aspiration, whereas some agents used to produce topical anaesthesia may raise the protein content of aqueous humour (Kronfeld, Lin & Luo 1941), as also do other pharmacological agents in topical use (Seidel 1920, Kadin 1963, Michiels 1964. The anaesthetic used in paracentesis therefore brings a potential source of error in protein studies.…”

“…Any irritation of the eye may, by axon reflexes, produce vasodilation and hence alterations in the chemical composition of the aqueous humour (Adler 1965). Kronfeld, Lin & Luo (1941) considered that, from the point of view of protein content, the instrument by which the bulb was fixed during paracenteslis did not matter, and that the paracentesis in itself did not affect the protein content. But if a failure is immediately followed by a successful paracentesis, the proteins are, however, nearly always increased in the specimen drawn (Kronfeld 1941).…”