The protein composition of reconstituted 30S ribosomal subunits: the effects of single protein omission

Absolute Requirement of Ammonium Sulfate for Reconstitution of Active 70s Ribosomes from the Extreme Halophilic Archaeon Haloferax Mediterranei

“…The development of in vitro reconstitution protocols for ribosomal subunits from different systems, in addition to proving that the ribosomal components have sufficient information to form fully active ribosomal particles, has made it possible to dissect the assembly processes and uncover the main rules governing the structure/function relationships between the different components (Traub and Nomura, 1968;Nomura and Erdmann, 1970;Nierhaus and Dohme, 1974;Amils et al, 1979;Londei et al, 1986;Moore et al, 1986;Sanchez et al, 1990;Buck et al, 1991). From a structural point of view, there are three main variables that are considered critical in the reconstitution processes; ionic strength (mainly responsible for the selection of specific interactions), the concentration of divalent cations (mainly involved in the conformation of the rRNA and total ribosomal RNA), and the temperature (energy).…”

Absolute Requirement of Ammonium Sulfate for Reconstitution of Active 70s Ribosomes from the Extreme Halophilic Archaeon Haloferax Mediterranei

“…The development of in vitro reconstitution protocols for ribosomal subunits from different systems, in addition to proving that the ribosomal components have sufficient information to form fully active ribosomal particles, has made it possible to dissect the assembly processes and uncover the main rules governing the structure/function relationships between the different components (Traub and Nomura, 1968;Nomura and Erdmann, 1970;Nierhaus and Dohme, 1974;Amils et al, 1979;Londei et al, 1986;Moore et al, 1986;Sanchez et al, 1990;Buck et al, 1991). From a structural point of view, there are three main variables that are considered critical in the reconstitution processes; ionic strength (mainly responsible for the selection of specific interactions), the concentration of divalent cations (mainly involved in the conformation of the rRNA and total ribosomal RNA), and the temperature (energy).…”

Absolute Requirement of Ammonium Sulfate for Reconstitution of Active 70s Ribosomes from the Extreme Halophilic Archaeon Haloferax Mediterranei

“…The development of in vitro reconstitution protocols for ribosomal subunits from different systems, in addition to proving that the ribosomal components have sufficient information to form fully active ribosomal particles, has made it possible to dissect the assembly processes and uncover the main rules governing the structure/function relationships between the different components (Traub and Nomura, 1968;Nomura and Erdmann, 1970;Nierhaus and Dohme, 1974;Amils et al, 1979;Londei et al, 1986;Moore et al, 1986;Sanchez et al, 1990;Buck et al, 1991). From a structural point of view, there are three main variables that are considered critical in the reconstitution processes; ionic strength (mainly responsible for the selection of specific interactions), the concentration of divalent cations (mainly involved in the conformation of the rRNA and total ribosomal RNA), and the temperature (energy).…”

Absolute Requirement of Ammonium Sulfate for Reconstitution of Active 70s Ribosomes from the Extreme Halophilic Archaeon Haloferax Mediterranei

“…The development of in vitro reconstitution protocols for ribosomal subunits from different systems, in addition to proving that the ribosomal components have sufficient information to form fully active ribosomal particles, has made it possible to dissect the assembly processes and uncover the main rules governing the structure/function relationships between the different components (Traub and Nomura, 1968;Nomura and Erdmann, 1970;Nierhaus and Dohme, 1974;Amils et al, 1979;Londei et al, 1986;Moore et al, 1986;Sanchez et al, 1990;Buck et al, 1991). From a structural point of view, there are three main variables that are considered critical in the reconstitution processes; ionic strength (mainly responsible for the selection of specific interactions), the concentration of divalent cations (mainly involved in the conformation of the rRNA and total ribosomal RNA), and the temperature (energy).…”