The protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish ( Takifugu obscurus ) under low temperature stress

Cheng, Chang-Hong; Liang, Heng; Luo, Sheng‐Wei; Wang, An‐Li; Ye, Chaoxia

doi:10.1016/j.jtherbio.2017.11.004

Cited by 29 publications

(18 citation statements)

References 66 publications

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“…Therefore, when UPase2 protein is up-regulated, it indicates that the catalytic reaction of uridine is strengthening, which leads to a decrease in uridine. Hence, in previous studies, when low-temperature stress was found to cause DNA damage in T. fasciatus [58], this might have been caused by an imbalance in uridine metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…For example, RBP was up-regulated in Channa striatus under high-temperature stress [63], suggesting that RBP has a positive effect on teleosts facing environmental temperature changes. In the vitamin digestion and absorption pathway, pantotherate (vitamin B5) and nicotinamide (vitamin B3) are up-regulated at low temperature, suggesting they might enhance low-temperature tolerance of T. fasciatus along with vitamin C [58]. Of course, the dosage of vitamins and their mechanism of action still need further study.…”

Section: Discussionmentioning

confidence: 99%

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Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus

Wen

Zhang

et al. 2019

BMC Genomics

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Background T. fasciatus ( Takifugu fasciatus ) faces the same problem as most warm water fish: the water temperature falls far below the optimal growth temperature in winter, causing a massive death of T. fasciatus and large economic losses. Understanding of the cold-tolerance mechanisms of this species is still limited. Integrated application of multi-omics research can provide a wealth of information to help us improve our understanding of low-temperature tolerance in fish. Results To gain a comprehensive and unbiased molecular understanding of cold-tolerance in T. fasciatus , we characterized mRNA-seq and metabolomics of T. fasciatus livers using Illumina HiSeq 2500 and UHPLC-Q-TOF MS. We identified 2544 up-regulated and 2622 down-regulated genes in the liver of T. fasciatus . A total of 40 differential metabolites were identified, including 9 down-regulated and 31 up-regulated metabolites. In combination with previous studies on proteomics, we have established an mRNA-protein-metabolite interaction network. There are 17 DEMs (differentially-expressed metabolites) and 14 DEGs-DEPs (differentially co-expressed genes and proteins) in the interaction network that are mainly involved in fatty acids metabolism, membrane transport, signal transduction, and DNA damage and defense. We then validated a number of genes in the interaction network by qRT-PCR. Additionally, a number of SNPs (single nucleotide polymorphisms) were revealed through the transcriptome data. These results provide key information for further understanding of the molecular mechanisms of T. fasciatus under cold stress. Conclusion The data generated by integrated application of multi-omics can facilitate our understanding of the molecular mechanisms of fish response to low temperature stress. We have not only identified potential genes and SNPs involved in cold tolerance, but also show that some nutrient metabolites may be added to the diet to help the overwintering of T. fasciatus . Electronic supplementary material The online version of this article (10.1186/s12864-019-5915-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus

Wen

Zhang

et al. 2019

BMC Genomics

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“…Consistent with its role in genome transcription, vitamin C was found to upregulate the expression of a series of genes that contribute to energy metabolism, immune responses, and cytoskeleton formation [34].…”

Section: Vitamin Cmentioning

confidence: 99%

Vitamin C as a Modulator of the Response to Cancer Therapy

et al. 2019

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Ascorbic acid (vitamin C) has been gaining attention as a potential treatment for human malignancies. Various experimental studies have shown the ability of pharmacological doses of vitamin C alone or in combinations with clinically used drugs to exert beneficial effects in various models of human cancers. Cytotoxicity of high doses of vitamin C in cancer cells appears to be related to excessive reactive oxygen species generation and the resulting suppression of the energy production via glycolysis. A hallmark of cancer cells is a strongly upregulated aerobic glycolysis, which elevates its relative importance as a source of ATP (Adenosine 5′-triphosphate). Aerobic glycolysis is maintained by a highly increased uptake of glucose, which is made possible by the upregulated expression of its transporters, such as GLUT-1, GLUT-3, and GLUT-4. These proteins can also transport the oxidized form of vitamin C, dehydroascorbate, permitting its preferential uptake by cancer cells with the subsequent depletion of critical cellular reducers as a result of ascorbate formation. Ascorbate also has a potential to affect other aspects of cancer cell metabolism due to its ability to promote reduction of iron(III) to iron(II) in numerous cellular metalloenzymes. Among iron-dependent dioxygenases, important targets for stimulation by vitamin C in cancer include prolyl hydroxylases targeting the hypoxia-inducible factors HIF-1/HIF-2 and histone and DNA demethylases. Altered metabolism of cancer cells by vitamin C can be beneficial by itself and promote activity of specific drugs.

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“…Moreover, it acts as a cofactor for many important mono-oxygenase and dioxygenase enzymes ( Spoelstra-de Man et al., 2018 ). Interestingly, VC has also been suggested to alleviate the side-effects of multiple stressors in animals as well as cell culture experiments (J. S. Mckee and Harrison, 1995 , Nosrati et al., 2017 , Cheng et al., 2018 , Yin et al., 2018 ). Chickens possess the ability to synthesize VC in kidneys through the glucuronatexylulose-xylulose cycle in the presence of L-gulonolactone oxidase ([EC 1.1.3.8], GLO ).…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with vitamin C ameliorates the adverse effects of Salmonella Enteritidis-challenge in broilers by shaping intestinal microbiota

et al. 2020

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Salmonella Enteritidis ( SE ) infection is not only a leading cause of poor production performance and compromised animal welfare in broilers but also a potential threat to public health. Two experiments were conducted to evaluate the effects of dietary supplemental vitamin C ( VC ) on SE challenged-broilers. In experiment 1, one hundred eighty 1-day-old Arbor Acre broilers were randomly allocated into 3 treatments, with 0, 500, or 1,000 mg/kg VC included in the diet. In experiment 2, dietary VC at 0 or 500 mg/kg, with or without SE challenge was applied in a 2 × 2 factorial arrangement in 6 randomized complete blocks. In experiment 1, addition with 500 mg/kg VC increased BW and infectious bursal disease ( IBD ) titer of broilers on 35 D ( P < 0.05), whereas 1,000 mg/kg VC had no effects on the IBD titer ( P > 0.05) compared with the control group. In experiment 2, SE challenge depressed BW on 11 and 21 D ( P < 0.05 and P = 0.088, respectively), whereas increased mortality and hepatic bacterial translocation ( P < 0.05) on 21 D. Further, SE challenge resulted in lower villus height in jejunum, lower microbial richness, and diversity, whereas higher abundance of Enterobacteriaceae in cecum ( P < 0.05). Importantly, supplementation with VC increased BW on both 21 and 35 D ( P < 0.05 and P = 0.088, respectively) and enhanced the intestinal health by improving villus morphology and microbial structure as indicated by higher cecal microbial richness and Firmicutes to Bacteroidetes ratio, while lower abundance of Enterobacteriaceae ( P < 0.05). In addition, birds fed with 500 mg/kg VC in the diet had significantly increased jejunal secretory immunoglobulin A levels, T lymphocytes stimulation index, and serum total antioxidant capability compared with groups without VC ( P < 0.05). In conclusion, SE challenge induced lower production performance and higher mortality in broilers. However, dietary supplementation with VC ameliorated SE-caused damage in broilers by improving the intestinal health, partly mediated by shaping the structure of cecal microbiota.

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The protective effects of vitamin C on apoptosis, DNA damage and proteome of pufferfish ( Takifugu obscurus ) under low temperature stress

Cited by 29 publications

References 66 publications

Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus

Integrated application of multi-omics provides insights into cold stress responses in pufferfish Takifugu fasciatus

Vitamin C as a Modulator of the Response to Cancer Therapy

Dietary supplementation with vitamin C ameliorates the adverse effects of Salmonella Enteritidis-challenge in broilers by shaping intestinal microbiota

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