The Protective Effects of Apigenin Against Radiation‐Induced Intestinal Injury

Liu, Danjie; Peng, Renjun; Chen, Zhongmin; Yu, Huijie; Wang, Sinian; Dong, Suhe; Li, Wei; Wen, Shifeng; Dai, Jing; Li, Fengsheng; Jiang, Qisheng; Sun, Wei

doi:10.1177/15593258221113791

Cited by 9 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resveratrol was reported to modulate the activity of antioxidant enzymes to alleviate oxidative stress damage through the NRF2 signaling pathway in the livers of ducks, resulting from a lipopolysaccharide-induced inflammatory response [60]. One study showed that apigenin decreased irradiation-induced oxidative stress in mice by increasing NRF2 protein expression [61], while another demonstrated that apigenin promoted the nuclear translocation of NRF2 and enhanced the protein expression level of NRF2, in addition to the expression of the NRF2 target gene NQO-1 [62]. The ZO family of proteins are key components of tight junctions.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Resveratrol and Apigenin on Jejunal Oxidative Injury in Ducks and on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2

Zhou,

Cao,

Luo

et al. 2024

Antioxidants

View full text Add to dashboard Cite

Oxidative stress increases the apoptosis of intestinal epithelial cells and impairs intestinal epithelial cell renewal, which further promotes intestinal barrier dysfunction and even death. Extensive evidence supports that resveratrol and apigenin have antioxidant, anti-inflammatory, and antiproliferative properties. Here, we investigated the ability of these two compounds to alleviate diquat-induced jejunal oxidative stress and morphological injury, using the duck as a model, as well as the effects of apigenin on oxidative stress induced by H2O2 in immortalized duck intestinal epithelial cells (IDECs). Ducks were randomly assigned to the following four groups, with five replicates: a control (CON) group, a diquat-challenged (DIQ) group, a resveratrol (500 mg/kg) + diquat (RES) group, and an apigenin (500 mg/kg) + diquat (API) group. We found that serum catalase (CAT) activity and total antioxidant capacity (T-AOC) markedly reduced in the RES and API groups as compared to the DIQ group (p < 0.05); moreover, serum S superoxide dismutase (SOD) levels increased significantly in the API group as compared to the DIQ group (p < 0.05). In jejunal mucosa, the malondialdehyde (MDA) content in the RES and API groups decreased more than that in the DIQ group (p < 0.05). In addition, the jejunal expression levels of the NRF2 and GCLM genes in the RES and API groups increased notably compared with those in the DIQ group (p < 0.05); meanwhile, CAT activity in the RES and API groups was markedly elevated compared with that in the CON group (p < 0.05). In IDECs, apigenin significantly restrained the H2O2-mediated increase in MDA content and decrease in CAT levels (p < 0.05). Furthermore, apigenin increased the protein expression of p-NRF2, NRF2, p-AKT, and p-P38; downregulated that of cleaved caspase-3 and cleaved caspase-9; and reduced the ratio of Bax/Bcl-2 in H2O2-treated IDECs (p < 0.05). In conclusion, resveratrol and apigenin can be used as natural feed additives to protect against jejunal oxidative stress in ducks.

show abstract

Section: Discussionmentioning

confidence: 99%

The Effects of Resveratrol and Apigenin on Jejunal Oxidative Injury in Ducks and on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2

Zhou,

Cao,

Luo

et al. 2024

Antioxidants

View full text Add to dashboard Cite

show abstract

“…Furthermore, CoAN is a crucial factor so that an agent can selectively kill tumor cells but with a protective effect on normal tissues. Interestingly, flavonoids have differential effects on normal and tumor tissues [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 ]. For instance, Api has been associated with a reduced risk of diabetes.…”

Section: Discussionmentioning

confidence: 99%

Synergism Antiproliferative Effects of Apigenin and Naringenin in NSCLC Cells

et al. 2023

View full text Add to dashboard Cite

Non-small cell lung cancer (NSCLC) is one of the leading cancer killers. Apigenin (Api) and Naringenin (Nar) are natural bioactive substances obtained in various vegetables and fruits, possessing anti-tumor effects across multiple studies. This study investigated the latent synergistic antiproliferative functions of Api and Nar in A549 and H1299 NSCLC cells. Cell viability was determined after incubating with different concentrations of Api, Nar, or the combination of Api and Nar (CoAN) for 24 h. Analysis using the CompuSyn software revealed that the CI value of each combined dose was < 1, depicting that the two drugs had a synergistic inhibitory effect. The CoAN (A:N = 3:2) group with the lowest CI value was selected for subsequent experiments. The IC50 of CoAN (A:N = 3:2) was used to determine the cell cycle, the expression ratio of Bax to Bcl2, Caspase 3 activity, and mitochondrial function to assess oxidative stress and apoptosis. The results established that CoAN treatment caused significant cytotoxicity with cell cycle arrest at G2/M phases. Furthermore, CoAN significantly enhanced mitochondria dysfunction, elevated oxidative stress, and activated the apoptotic pathway versus Api or Nar alone groups. Thus, the CoAN chemotherapy approach is promising and deserves further research.

show abstract

“…Table 3. 4 shows the binding energies and Ki of apigenin and related flavonoids with DNA gyrase B(PDB:6F86 and PDB:4PRV) and FABZ(PDB:3CF9 site A and site B). Our results show that apigenin and other flavonoids are capable of binding to DNA gyrase at both sites, but are more potent in binding to the PDB:4PRV site than PDB:6F86.…”

Section: Binding Energies and Inhibition Constants (Ki) Of Apigenin A...mentioning

confidence: 99%

In silico comparison of apigenin and related compounds with diverse pharmacological activities using AutoDock 4.2.6

Peanlikhit,

Aryal,

Welsh

et al. 2024

Preprint

View full text Add to dashboard Cite

In recent years, molecular docking has been used to predict the binding affinity and interaction between flavonoids (ligands) and specific protein targets (receptors). However, existing studies have focused on a limited number of flavonoids with a small number of targeted proteins. In this study, on the other hand, we used the AutoDock 4.2.6 molecular docking software to compare the binding energy values and interactions of apigenin and 15 flavonoids with 24 different proteins known to be associated with oxidative stress, inflammation, carcinogenesis, and bacterial infections. This comparison will be important for the development of flavonoid-based drugs, allowing for the optimization of flavonoid drugs to better interact with their target proteins alone or in combination with conventional drugs. We used three-dimensional crystal structures of the receptors to identify the amino acids in the active site for docking purposes. Our goal was to identify potential inhibitors of these proteins and understand the structural configurations of flavonoids that affect their binding energy (binding affinity or inhibitory effects). We found that apigenin was a highly effective inhibitor for all tested proteins, with the highest binding energy of -8.21 kcal/mol with p38 mitogen-activated protein kinases and the lowest binding energy of -5.34 kcal/mol with cyclin-dependent kinase 4. In fact, apigenin and most of the tested flavonoids were better inhibitors for xanthine oxidase or DNA methyltransferase than known inhibitors. Our results also suggest that apigenin and selected flavonoids could be used to inhibit the oncogenic activity of KRAS mutations at codon 12 (i.e., G12C, G12D, and G12V) frequently found in solid tumors since they possess high binding energies with these mutated proteins. This finding offers an advantage over targeted therapy drugs that focus on specific KRAS mutations. Furthermore, most flavonoids have good safety profiles, so our resulting data are crucial for developing flavonoid-based drugs and present new insights into developing effective treatments that can attenuate the resistance and side effects of chemotherapy.

show abstract

The Protective Effects of Apigenin Against Radiation‐Induced Intestinal Injury

Cited by 9 publications

References 26 publications

The Effects of Resveratrol and Apigenin on Jejunal Oxidative Injury in Ducks and on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2

The Effects of Resveratrol and Apigenin on Jejunal Oxidative Injury in Ducks and on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2

Synergism Antiproliferative Effects of Apigenin and Naringenin in NSCLC Cells

In silico comparison of apigenin and related compounds with diverse pharmacological activities using AutoDock 4.2.6

Contact Info

Product

Resources

About