The Protective Effect of <i>Cassia obtusifolia</i> on DSS-Induced Colitis

Kim, Sujin; Kim, Koh-Woon; Kim, Daeseung; Kim, Min‐Cheol; Jeon, Yong-Deok; Kim, Se-Gun; Jung, Hyun‐Ju; Jang, Hyeung-Jin; Lee, Byung-Cheol; Chung, Won-Seok; Hong, Seung‐Heon; Chung, Seok-Hee; Um, Jae‐Young

doi:10.1142/s0192415x11009032

Cited by 27 publications

(20 citation statements)

References 43 publications

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“…The inflammatory bowel disease (IBD), including ulcerative colitis and Crohn’s disease, is associated with colonic mucosal ulceration, shortening of the colon, diarrhea, blood in the feces, and weight loss (Fiocchi, 1998; Kim et al , 2011; Kohn and Meddi, 2012). It is well known that chronic inflammation in the colon can lead to cancer in humans (Hanauer, 1996; Geier et al , 2007).…”

Section: Introductionmentioning

confidence: 99%

Salvia miltiorrhiza (Dan Shen) Significantly Ameliorates Colon Inflammation in Dextran Sulfate Sodium Induced Colitis

Wen

Wang

et al. 2013

Am. J. Chin. Med.

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Inflammatory bowel disease increases the risks of human colorectal cancer. In this study, the effects of Salvia miltiorrhiza extract (SME) on chemically-induced colitis in a mouse model were evaluated. Chemical composition of SME was determined by HPLC analysis. A/J mice received a single injection of AOM 7.5 mg/kg. After one week, these mice received 2.5% DSS for 8 days, or DSS plus SME (25 or 50 mg/kg). DSS-induced colitis was scored with the disease activity index (DAI). Body weight and colon length were also measured. The severity of inflammatory lesions was further evaluated by colon tissue histological assessment. HPLC assay showed that the major constituents in the tested SME were danshensu, protocatechuic aldehyde, salvianolic acid D and salvianolic acid B. In the model group, the DAI score reached its highest level on Day 8, while the SME group on both doses showed a significantly reduced DAI score (both P < 0.01). As an objective index of the severity of inflammation, colon length was reduced significantly from the vehicle group to model group. Treatment with 25 and 50 mg/kg of SME inhibited the reduction of colon in a dose-related manner (P < 0.05 and P < 0.01, respectively). SME groups also significantly reduced weight reduction (P < 0.05). Colitis histological data supported the pharmacological observations. Thus, Salvia miltiorrhiza could be a promising candidate in preventing and treating colitis and in reducing the risks of inflammation-associated colorectal cancer.

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Section: Introductionmentioning

confidence: 99%

Salvia miltiorrhiza (Dan Shen) Significantly Ameliorates Colon Inflammation in Dextran Sulfate Sodium Induced Colitis

Wen

Wang

et al. 2013

Am. J. Chin. Med.

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“…COX-2 is involved in a variety of pathological processes and regulates the production of prostaglandins, which can promote cell growth, angiogenesis, and immunosuppression. [30] In chronic inflammation, nitric oxide stimulates COX-2 activity and reduces p53 expression, thereby contributing to clone cellular expansion and genomic instability. [31] The mechanism by which meju prevents colitis-associated colon carcinogenesis may specifically involve iNOS and COX-2.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of meju prepared with mixed starter cultures on azoxymethane and dextran sulfate sodium-induced colon carcinogenesis in mice

Jeong¹,

Chang²,

Park³

2012

J Carcinog

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Backgrounds:Meju is the main ingredient and the starter culture of traditional Korean fermented soybean foods; these fermented soybean products are well-known for their various health benefits, including anticancer effects. We developed the grain-type meju using probiotic mixed starter cultures to improve the qualities and functionalities of fermented soybean products, as well as the meju itself. In this study, the inhibitory effects of the grain-type meju were investigated in azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colon carcinogenesis mice model.Materials and Methods:AOM and DSS colon carcinogenesis was induced in female C57BL/6 mice and meju was orally administered for 4 weeks. The body weight, colon length, and colon weight of mice were determined, and colonic tissues were histologically observed. The serum levels of proinflammatory cytokines and the levels of inflammation- and apoptosis-related genes in colonic tissue were also analyzed.Results:The administration of meju using probiotic mixed starter cultures ameliorated the symptoms of colon cancer and reduced number of neoplasia, and reduced serum proinflammatory cytokine levels and iNOS and COX-2 expression levels in colonic tissue. It increased Bax and reduced Bcl-2 expression levels and increased p21 and p53 expression in colonic tissues.Conclusion:The meju showed inhibitory effects on the progression of colon cancer induced by AOM and DSS by ameliorating the symptoms of colon cancer, reducing the number of neoplasias and regulating proinflammatory cytokine levels and the expressions of inflammation- and apoptosis-related genes in the colonic tissue.

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“…It has been widely used as medicinal herb for the treatment of headache, dizziness, dysentery, and eye disease for centuries. 17) Several classes of bioactive metabolites have been identified from C. obtusifolia, including anthraquinone, 18) terpenoid, 19) flavonoid, 20) and lipid, 21) etc. According to the Chinese Pharmacopoeia, aurantio-obtusin, 22) a kind of anthraquinone, was identified as the characteristic bioactive component in C. obtusifolia and Cassia tora.…”

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confidence: 99%

Selection and evaluation of reference genes for expression analysis of Cassi

Liu

Zhu

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Cassia obtusifolia, belonging to legume family, is important in many fields with high pharmaceutical, economic, and ecological values. These interests of C. obtusifolia triggered in-depth and fundamental genetic and molecular research. Therefore, the stable reference gene is necessary for normalization of the gene expression studies. In this study, 10 candidate reference genes were subjected to expression analysis in 12 different tissues and under different stresses by qRT-PCR. The expression stability was evaluated using geNorm, NormFinder, and BestKeeper software. In conclusion, different suitable reference genes were selected in different tissues and under different stress. CYP1, EF1α2, ACT2, UBQ1 were the most stable reference genes in all samples. The relative expression levels of WRKY gene were detected to confirm the reliability of the selected reference genes. These results provided suitable reference genes that could be used for normalization in C. obtusifolia tissues and under different stress.

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The Protective Effect of Cassia obtusifolia on DSS-Induced Colitis

Cited by 27 publications

References 43 publications

Salvia miltiorrhiza (Dan Shen) Significantly Ameliorates Colon Inflammation in Dextran Sulfate Sodium Induced Colitis

Salvia miltiorrhiza (Dan Shen) Significantly Ameliorates Colon Inflammation in Dextran Sulfate Sodium Induced Colitis

Inhibitory effects of meju prepared with mixed starter cultures on azoxymethane and dextran sulfate sodium-induced colon carcinogenesis in mice

Selection and evaluation of reference genes for expression analysis of Cassi

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