The promoter of the recA gene of Escherichia coli

Identification of genetic interactions with priB links the PriA/PriB DNA replication restart pathway to double-strand DNA break repair in Escherichia coli

“…In addition to restricting the scope of RecA activity in vivo with a recR deletion, we hypothesized that reducing the cellular levels of RecA would also produce a suppressive effect. To accomplish this, we utilized a recA promoter mutation, P recA (AtoG), which decreases recA expression [43, 67, 68]. This mutation also suppressed the effects of lexA or rdgC mutations in Δ priB cells, and the degree of suppression was strikingly similar to that of a recR deletion (Fig 7D).…”

Identification of genetic interactions with priB links the PriA/PriB DNA replication restart pathway to double-strand DNA break repair in Escherichia coli

“…In addition to restricting the scope of RecA activity in vivo with a recR deletion, we hypothesized that reducing the cellular levels of RecA would also produce a suppressive effect. To accomplish this, we utilized a recA promoter mutation, P recA (AtoG), which decreases recA expression [43, 67, 68]. This mutation also suppressed the effects of lexA or rdgC mutations in Δ priB cells, and the degree of suppression was strikingly similar to that of a recR deletion (Fig 7D).…”

From macro to micro: a combined bioluminescence‐fluorescence approach to monitor bacterial localization

“…purpose of the experiment, equipment used to detect luminescence). While we would expect the constructs to give different expression levels in different bacterial strains, the constructs should maintain a wide expression range as the OXB promoters (Oxford Genetics Limited, UK) were all derived from the constitutive and conserved recA promoter (Weisemann and Weinstock, 1991 ).…”

Resolving Toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD

“…The base change is a T to C in the first position of the six-nucleotide Pribnow box sequence (Figure 4B ). Mutations in this position will result in reduced expression of recA ( 43 , 44 ). RecA is the central recombinase in E. coli that facilitates homologous pairing in double strand break and daughter strand gap repair ( 45 , 46 ).…”

Identification of genetic interactions with priB links the PriA/PriB DNA replication restart pathway to double-strand DNA break repair in Escherichia coli

“…In addition to restricting the scope of RecA activity in vivo with a recR deletion, we hypothesized that reducing the cellular levels of RecA would also produce a suppressive effect. To accomplish this, we utilized a recA promoter mutation, P recA (AtoG), which decreases recA expression [43, 67, 68]. This mutation also suppressed the effects of lexA or rdgC mutations in Δ priB cells, and the degree of suppression was strikingly similar to that of a recR deletion (Fig 7D).…”

From macro to micro: a combined bioluminescence‐fluorescence approach to monitor bacterial localization

“…purpose of the experiment, equipment used to detect luminescence). While we would expect the constructs to give different expression levels in different bacterial strains, the constructs should maintain a wide expression range as the OXB promoters (Oxford Genetics Limited, UK) were all derived from the constitutive and conserved recA promoter (Weisemann and Weinstock, 1991 ).…”

Resolving Toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD

“…The base change is a T to C in the first position of the six-nucleotide Pribnow box sequence (Figure 4B ). Mutations in this position will result in reduced expression of recA ( 43 , 44 ). RecA is the central recombinase in E. coli that facilitates homologous pairing in double strand break and daughter strand gap repair ( 45 , 46 ).…”

Identification of genetic interactions with priB links the PriA/PriB DNA replication restart pathway to double-strand DNA break repair in Escherichia coli

“…In addition to restricting the scope of RecA activity in vivo with a recR deletion, we hypothesized that reducing the cellular levels of RecA would also produce a suppressive effect. To accomplish this, we utilized a recA promoter mutation, P recA (AtoG), which decreases recA expression [43, 67, 68]. This mutation also suppressed the effects of lexA or rdgC mutations in Δ priB cells, and the degree of suppression was strikingly similar to that of a recR deletion (Fig 7D).…”

From macro to micro: a combined bioluminescence‐fluorescence approach to monitor bacterial localization

“…purpose of the experiment, equipment used to detect luminescence). While we would expect the constructs to give different expression levels in different bacterial strains, the constructs should maintain a wide expression range as the OXB promoters (Oxford Genetics Limited, UK) were all derived from the constitutive and conserved recA promoter (Weisemann and Weinstock, 1991 ).…”

Resolving Toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD

“…The base change is a T to C in the first position of the six-nucleotide Pribnow box sequence (Figure 4B ). Mutations in this position will result in reduced expression of recA ( 43 , 44 ). RecA is the central recombinase in E. coli that facilitates homologous pairing in double strand break and daughter strand gap repair ( 45 , 46 ).…”