The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis

Hutchings, Greg; Janowicz, Krzysztof; Moncrieff, Lisa; Dompe, Claudia; Strauss, Ewa; Kocherova, Ievgeniia; Nawrocki, Mariusz J.; Kruszyna, Łukasz; Wąsiatycz, Grzegorz; Antosik, Paweł; Shibli, Jamil Awad; Mozdziak, Paul; Perek, Bartłomiej; Krasiński, Zbigniew; Kempisty, Bartosz; Nowicki, Michał

doi:10.3390/ijms21113790

Cited by 59 publications

(67 citation statements)

References 174 publications

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“…In contrast, dysregulated adipogenesis by ROS was demonstrated by increased proliferation of adipose-derived stem cells (ASCs) and preadipocyte differentiation [105,106], followed by a reduction of NOX4 content [107,108]. Moreover, hypoxia induces increased ASCs expression of vascular endothelial growth factor (VEGF), which in turn stimulates adipocyte proliferation [109]. Noteworthy, non-coordinated adipogenesis and angiogenesis may result in the impairment of recruitment and differentiation of ASCs, contributing to adipocyte hypertrophy [110].…”

Section: Obesity-induced At Dysfunction and Oxidative Stressmentioning

confidence: 99%

Contribution of Adipose Tissue Oxidative Stress to Obesity-Associated Diabetes Risk and Ethnic Differences: Focus on Women of African Ancestry

et al. 2021

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Adipose tissue (AT) storage capacity is central in the maintenance of whole-body homeostasis, especially in obesity states. However, sustained nutrients overflow may dysregulate this function resulting in adipocytes hypertrophy, AT hypoxia, inflammation and oxidative stress. Systemic inflammation may also contribute to the disruption of AT redox equilibrium. AT and systemic oxidative stress have been involved in the development of obesity-associated insulin resistance (IR) and type 2 diabetes (T2D) through several mechanisms. Interestingly, fat accumulation, body fat distribution and the degree of how adiposity translates into cardio-metabolic diseases differ between ethnicities. Populations of African ancestry have a higher prevalence of obesity and higher T2D risk than populations of European ancestry, mainly driven by higher rates among African women. Considering the reported ethnic-specific differences in AT distribution and function and higher levels of systemic oxidative stress markers, oxidative stress is a potential contributor to the higher susceptibility for metabolic diseases in African women. This review summarizes existing evidence supporting this hypothesis while acknowledging a lack of data on AT oxidative stress in relation to IR in Africans, and the potential influence of other ethnicity-related modulators (e.g., genetic-environment interplay, socioeconomic factors) for consideration in future studies with different ethnicities.

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Section: Obesity-induced At Dysfunction and Oxidative Stressmentioning

confidence: 99%

Contribution of Adipose Tissue Oxidative Stress to Obesity-Associated Diabetes Risk and Ethnic Differences: Focus on Women of African Ancestry

et al. 2021

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“…In addition, tissue-derived stem cells have a degree of plasticity, depending on their type. This manifests in the differentiation phenotypic potential that goes beyond the cell phenotype of their original tissue [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Adipose Tissue-Derived Stem Cells: The Biologic Basis and Future Directions for Tissue Engineering

Câmara¹,

Shibli

Müller

et al. 2020

Materials

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Mesenchymal stem cells (MSCs) have been isolated from a variety of tissues using different methods. Active research have confirmed that the most accessible site to collect them is the adipose tissue; which has a significantly higher concentration of MSCs. Moreover; harvesting from adipose tissue is less invasive; there are no ethical limitations and a lower risk of severe complications. These adipose-derived stem cells (ASCs) are also able to increase at higher rates and showing telomerase activity, which acts by maintaining the DNA stability during cell divisions. Adipose-derived stem cells secret molecules that show important function in other cells vitality and mechanisms associated with the immune system, central nervous system, the heart and several muscles. They release cytokines involved in pro/anti-inflammatory, angiogenic and hematopoietic processes. Adipose-derived stem cells also have immunosuppressive properties and have been reported to be “immune privileged” since they show negative or low expression of human leukocyte antigens. Translational medicine and basic research projects can take advantage of bioprinting. This technology allows precise control for both scaffolds and cells. The properties of cell adhesion, migration, maturation, proliferation, mimicry of cell microenvironment, and differentiation should be promoted by the printed biomaterial used in tissue engineering. Self-renewal and potency are presented by MSCs, which implies in an open-source for 3D bioprinting and regenerative medicine. Considering these features and necessities, ASCs can be applied in the designing of tissue engineering products. Understanding the heterogeneity of ASCs and optimizing their properties can contribute to making the best therapeutic use of these cells and opening new paths to make tissue engineering even more useful.

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“…Pathologic neovascularization is characterized by proliferation and migration of abnormal cells [ 19 , 20 ]. In this study, we showed that in vivo, PSF ameliorated RNV and corrected aberrant VEGF expression in an OIR mouse model and reduced IRNV in Vldlr − / − mice.…”

Section: Discussionmentioning

confidence: 99%

PSF functions as a repressor of hypoxia-induced angiogenesis by promoting mitochondrial function

Dong

Lin

et al. 2021

Cell Commun Signal

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Background Abnormal neovascularization is the most common cause of blindness, and hypoxia alters tissue metabolism, function, and morphology. HIF-1α, the transcriptional activator of VEGF, has intricate mechanisms of nuclear translocation and activation, but its signal termination mechanisms remain unclear. Methods We investigated the role of polypyrimidine tract-binding protein-associated splicing factor (PSF) in cellular energy production, migration, and proliferation by targeting HIF-1α in vivo and in vitro PSF plasmids were transfected with liposome 2000 transfection reagent. Young C57/BL6J mice were kept in a hyperoxia environment, followed by indoor air, resulting in oxygen-induced retinopathy. Oxygen-induced retinopathy (OIR) animals were randomly divided into three groups: OIR group, OIR + vector group (OIR cubs treated with rAAV vector) and OIR + PSF group (OIR cubs treated with rAAV-PSF). Age-matched C57/BL6J mice were used as controls and exposed to constant normoxic conditions. The animals were executed and their pupils were subjected to subsequent experiments. The metabolic spectrum was analyzed by Seahorse XFe96 flux analyzer, and OCR and extracellular acidification rate were quantified at the same time. Results PSF ameliorated retinal neovascularization and corrected abnormal VEGF expression in mice with oxygen-induced retinopathy and reduced intra-retinal neovascularization in Vldlr − / − mice. PSF reprogrammed mitochondrial bioenergetics and inhibited the transition of endothelial cells after hypoxia, suggesting its involvement in pathological angiogenesis.Ectopic PSF expression inhibited hypoxia-induced HIF-1α activation in the nucleus by recruiting Hakai to the PSF/HIF-1α complex, causing HIF-1α inhibition. PSF knockdown increased hypoxia-stimulated HIF-1α reactions. These hypoxia-dependent processes may play a vital role in cell metabolism, migration, and proliferation. Thus, PSF is a potential treatment target in neovascularization-associated ophthalmopathy. Conclusion This is the first study showing that PSF inhibits HIF-1α via recruitment of Hakai, modulates mitochondrial oxidation and glycolysis, and downregulates VEGF expression under hypoxia. We propose a new HIF-1 α/Hakai regulatory mechanism that may play a vital role in the pathogenesis of neovascularization in ophthalmopathy. PSF-Hakai–HIF-1α signaling pathway under hypoxia condition. Schematic diagram showing that the PSF-Hakai–HIF-1α signaling pathway. Under hypoxia condition, PSF-Hakai complex regulate HIF-1α signaling, thus inhibiting downstream target gene VEGF, cell metabolism and angiogenesis eventually.

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The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis

Cited by 59 publications

References 174 publications

Contribution of Adipose Tissue Oxidative Stress to Obesity-Associated Diabetes Risk and Ethnic Differences: Focus on Women of African Ancestry

Contribution of Adipose Tissue Oxidative Stress to Obesity-Associated Diabetes Risk and Ethnic Differences: Focus on Women of African Ancestry

Adipose Tissue-Derived Stem Cells: The Biologic Basis and Future Directions for Tissue Engineering

PSF functions as a repressor of hypoxia-induced angiogenesis by promoting mitochondrial function

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