The production of nitric oxide and TNF-α in peritoneal macrophages is inhibited by Dichroa febrifuga Lour

Dichroa febrifuga Lour. inhibits the production of IL-1β and IL-6 through blocking NF-κB, MAPK and Akt activation in macrophages

“…These cytokines play pivotal roles in the induction of the innate immune response as well as in the determination of the magnitude and nature (Th1 vs Th2) of the acquired immune response (Netea et al, 2003). We have previously demonstrated that AEDF exhibits anti-inflammatory function by inhibiting the production of NO and TNF-␣ in macrophages (Kim et al, 2000). Our results in the present investigation are consistent with our previous data, and these results suggest that AEDF acts as anti-inflammatory agent influencing these proinflammatory cytokines and NO.…”

“…Thioglycollate (TG) broth (Brewer, DIFCO, Detroit, MI)-elicited macrophages were harvested 3 days after intraperitoneal injection of 2.5 ml TG into mice and isolated as reported previously (Kim et al, 2000). Murine macrophage RAW 264.7 cells were maintained in Dulbecco's modified Eagle's medium supplemented with glutamine (1 mM) and 10% FBS.…”

“…Halofuginone is also reported to inhibit NF-B and p38 MAPK in activated T cells (Leiba et al, 2006) and ameliorate trinitrobenzene sulfonic acid (TNBS)-induced colonic inflammation (Karakoyun et al, in press). According to our previous study, the aqueous extract from Dichroa febrifuga root (AEDF) suppresses the endotoxin-induced inflammatory responses through inhibiting the production of NO in vivo and in vitro (Kim et al, 2000). But, the underlying mechanism of AEDF function has remained to be characterized so far.…”

Hydrangeaceae

“…Roots of Dichroa febrifuga have been used as a traditional source of antimalarial drugs in Asia (Fairbairn and Lou 1950;Steinmetz 1972;Murata et al1998;Kim et al 2000). Roots of Dichroa febrifuga have been used as a traditional source of antimalarial drugs in Asia (Fairbairn and Lou 1950;Steinmetz 1972;Murata et al1998;Kim et al 2000).…”

Biochemistry and Herbs

“…Lipopolysaccharide (LPS)-stimulated macrophage cell line with detection of NO production is one of the in vitro models used to identify anti-inflammatory potential substances (Ismaili et al, 2002;Kim et al, 2000). There are several inflammatory animal models for different degrees and types of inflammatory responses.…”