The product of BMP-directed differentiation protocols for human primed pluripotent stem cells is placental trophoblast and not amnion

Seetharam, Arun S.; Vu, Ha; Choi, Sehee; Khan, Teka; Sheridan, Megan A.; Ezashi, Toshihiko; Roberts, R. Michael; Tuteja, Geetu

doi:10.1016/j.stemcr.2022.04.014

Cited by 16 publications

(12 citation statements)

References 48 publications

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“…Subsequently, we performed GSEA using an amnion gene list obtained through a more stringent analysis of the one by Roost et al. (2015) , as performed by Seetheram et al. (2022) ( Figure 2 F).…”

Section: Resultsmentioning

confidence: 99%

Derivation of functional trophoblast stem cells from primed human pluripotent stem cells

et al. 2022

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Section: Resultsmentioning

confidence: 99%

Derivation of functional trophoblast stem cells from primed human pluripotent stem cells

et al. 2022

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“…( 30 ) and Castel et al ( 31 ) were obtained from Seetharam et al . ( 48 ). The data from Liu et al .…”

Section: Methodsmentioning

confidence: 99%

Conservation at the uterine-placental interface

Scott

Jain

et al. 2022

Preprint

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The hemochorial placentation site is characterized by a dynamic interplay between trophoblast cells and maternal cells. These cells cooperate to establish an interface required for nutrient delivery to promote fetal growth. In the human, trophoblast cells penetrate deep into the uterus. This is not a consistent feature of hemochorial placentation and has hindered the establishment of suitable animal models. The rat represents an intriguing model for investigating hemochorial placentation with deep trophoblast cell invasion. In this study, we used single cell RNA sequencing to characterize the transcriptome of the invasive trophoblast cell lineage, as well as other cell populations within the rat uterine-placental interface during early (gestation day, gd, 15.5) and late (gd 19.5) stages of intrauterine trophoblast cell invasion. We identified a robust set of transcripts that define invasive trophoblast cells, as well as transcripts that distinguished endothelial, smooth muscle, natural killer, and macrophage cells. Invasive trophoblast, immune, and endothelial cell populations exhibited distinct spatial relationships within the uterine-placental interface. Furthermore, the maturation stage of invasive trophoblast cell development could be determined by assessing gestation-stage dependent changes in transcript expression. Finally, and most importantly, expression of a prominent subset of rat invasive trophoblast cell transcripts is conserved in the invasive extravillous trophoblast cell lineage of the human placenta. These findings provide foundational data to identify and interrogate key conserved regulatory mechanisms essential for development and function of an important compartment within the hemochorial placentation site that is essential for a healthy pregnancy.

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“…It is therefore somewhat surprising that many reports have confirmed that these archetypal hPSCs are able to give rise to amnion and primordial germ cells, since these are lineages thought to arise from the post-implantation epiblast very soon after implantation (review, Pera and Rossant, 2021 ). A cohort of recent publications, including two in this issue of Stem Cell Reports ( Soncin et al., 2022 ; Seetharam et al., 2022 ), raises further fascinating questions concerning the developmental potential of archetypal hPSCs.…”

Section: Main Textmentioning

confidence: 99%

“… Seetharam et al (2022) surveyed RNA sequencing (RNA-seq) data from three published BMP differentiation studies and compared them using a placenta database and data from blastocyst and early embryo cultures. They performed principal component analysis across a range of experimental models of trophoblast and amnion cells, and carried out cluster analysis on single-cell RNA-seq (scRNA-seq) studies of hPSC differentiation.…”

Section: Main Textmentioning

confidence: 99%

Pluripotent cell states and unexpected fates

Pera

2022

Stem Cell Reports

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The product of BMP-directed differentiation protocols for human primed pluripotent stem cells is placental trophoblast and not amnion

Cited by 16 publications

References 48 publications

Derivation of functional trophoblast stem cells from primed human pluripotent stem cells

Derivation of functional trophoblast stem cells from primed human pluripotent stem cells

Conservation at the uterine-placental interface

Pluripotent cell states and unexpected fates

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