The prevalence of microsatellite instability in head and neck squamous cell carcinoma

Schutter, Harlinde De; Spaepen, Marijke; Opstal, S Van; Poorten, Vincent Vander; Verbeken, Erik; Nuyts, Sandra

doi:10.1007/s00432-008-0476-1

Cited by 13 publications

(11 citation statements)

References 11 publications

(11 reference statements)

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“…We found 37.9% of head and neck squamous cell carcinoma specimens had MSI, whereas 58.6% of them had LOH in at least one marker. The frequency of MSI reported in the literature ranged from 1.25 to 88% while the frequency of LOH varied from 26 to 100% 6 7 9 10 11 12 13 15 16 17 . The diverse frequency in microsatellite alteration might be explained by the different microsatellite markers selected.…”

Section: Discussionmentioning

confidence: 99%

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Microsatellite alteration in head and neck squamous cell carcinoma patients from a betel quid-prevalent region

Lin

Wang

Jiang

et al. 2016

Sci Rep

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We investigated the frequency of microsatellite alteration and their impact on survival in head and neck squamous cell carcinoma patients from an endemic betel quid chewing area. We collected 116 head and neck squamous cell carcinoma specimens along with corresponding surgical margins which were confirmed by pathological examination. Ten oligonucleotide markers were chosen for the assessment of microsatellite alteration. The specimens were amplified by polymerase chain reaction followed by automatic fragment analysis. There were 44 specimens (37.9%) with microsatellite instability (MSI) in at least one marker while more than half of the specimens (n = 68, 58.6%) had loss of heterozygosity (LOH) in at least one marker. Though MSI/LOH was not correlated with the survival of head and neck squamous cell carcinoma patients, presence of MSI in the tumor-free surgical margins was associated with local recurrence (odds ratio: 15.14; 95% confidence interval: 6.451 ~ 35.53; P < 0.001). Genomic assessment of surgical margin can help surgeons to identify head and neck squamous cell carcinoma patients who are at risk of developing local recurrence in a betel quid-prevalent region.

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Section: Discussionmentioning

confidence: 99%

“…De Schutter et al . even found only one out of 80 tumors could be considered positive for MSI in advanced head and neck cancer patients 10 . Furthermore, MSI in the surgical margin was found to increase the likelihood of local recurrence in head and neck squamous cell carcinoma patients 7 .…”

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confidence: 99%

Microsatellite alteration in head and neck squamous cell carcinoma patients from a betel quid-prevalent region

Lin

Wang

Jiang

et al. 2016

Sci Rep

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“…Since microsatellite instability appears to have rather low prevalence in HNSCC (De Schutter et al, 2009), we assumed that the examination of multiple methylation of tumorrelated genes could provide important information on oral cancer etiology and progression.…”

Section: Discussionmentioning

confidence: 99%

Interaction between the MTHFR C677T Polymorphism and Alcohol—Impact on Oral Cancer Risk and Multiple DNA Methylation of Tumor-related Genes

et al. 2010

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Several studies have reported Oral Squamous Cell Carcinoma (OSCC) association with etiological factors, such as smoking and alcohol. The aim of the present study was to establish whether the methylenetetrahydrofolate reductase (MTHFR) C677T genotype and a high alcohol intake, solely or in interaction, have an impact on the oral cancer risk, DNA methylation, or multiple methylation of tumor-related genes. MTHFR C677T genetic polymorphism was determined by the PCR/RFLP method, and DNA methylation was assessed by nested methylation-specific PCR. The risk for multiple methylation was significantly increased in heavy-drinking patients with the TT genotype, compared with CC and CT patients (OR = 10.873; 95% CI, 1.134-104.24). Multiple methylation was significantly associated with tumor stage (p = 0.018), and showed a trend of association with the presence of nodal metastases (p = 0.058). A significant association was found between TT genotype and methylation status of the RASSF1A gene in OSCC patients (p = 0.012). Heavy-drinking individuals with the TT genotype showed increased oral cancer risk compared with the CC genotype (OR = 3.601; 95% CI, 1.036-12.513), and compared with the CC and CT genotypes (OR = 4.288; 95% CI, 1.325-13.877). Our study suggested gene-environment interactions between high alcohol intake and the MTHFR 677TT genotype for elevated oral cancer risk, with a significant impact on multiple methylation of cancer-related genes.

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“…MIN, which is known to be a genetic phenotype of non-polyposis colorectal cancer, is observed at the nucleotide level [3]. In HNSCC, the reported frequencies of MIN vary from 1.23 [4,5] to 57.9% [6]; in general the incidence is relatively low. In contrast, in most solid tumors including oral SCCs, CIN occurs at the chromosomal level, with frequent gains and losses of whole chromosomes or chromosomal segments.…”

Section: Introductionmentioning

confidence: 99%

Prognostic utility of chromosomal instability detected by fluorescence in situ hybridization in fine-needle aspirates from oral squamous cell carcinomas

et al. 2010

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BackgroundAlthough chromosomal instability (CIN) has been detected in many kinds of human malignancies by means of various methods, there is no practical assessment for small clinical specimens. In this study, we evaluated CIN in fine-needle aspiration (FNA) biopsied oral squamous cell carcinomas (SCCs) using fluorescence in situ hybridization (FISH) analysis, and investigated its prognostic significance.MethodsTo evaluate CIN status of tumors, FISH with genomic probes for the centromeres of chromosomes 7, 9, and 11 was performed on specimens obtained by FNA from 77 patients with primary oral SCCs.ResultsHigh-grade CIN (CIN3) was observed in 11.7% (9/77) of patients with oral SCCs and was associated significantly with reduced disease-free survival (p = .008) and overall survival (p = .003). Multivariate Cox proportional hazards analysis showed that CIN status was significantly correlated with disease-free survival (p = .035) and overall survival (p = .041).ConclusionAnalysis of CIN status using FISH on FNA biopsy specimens may be useful in predicting of recurrence and poor prognosis in patients with oral SCCs.

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The prevalence of microsatellite instability in head and neck squamous cell carcinoma

Abstract: The present results indicate that MSI has a low prevalence in HNSCC.

Cited by 13 publications

References 11 publications

Microsatellite alteration in head and neck squamous cell carcinoma patients from a betel quid-prevalent region

Microsatellite alteration in head and neck squamous cell carcinoma patients from a betel quid-prevalent region

Interaction between the MTHFR C677T Polymorphism and Alcohol—Impact on Oral Cancer Risk and Multiple DNA Methylation of Tumor-related Genes

Prognostic utility of chromosomal instability detected by fluorescence in situ hybridization in fine-needle aspirates from oral squamous cell carcinomas

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