The predominant bacteria isolated from radicular cysts

Abstract

PurposeTo detect predominant bacteria associated with radicular cysts and discuss in light of the literature.Material and methodsClinical materials were obtained from 35 radicular cysts by aspiration. Cultures were made from clinical materials by modern laboratory techniques, they underwent microbiologic analysis.ResultsThe following are microorganisms isolated from cultures: Streptococcus milleri Group (SMG) (23.8%) [Streptococcus constellatus (19.1%) and Streptococcus anginosus (4.7%)], Streptococcus sanguis (14.3%), Streptococcus mitis (4.7%), Streptococcus cremoris (4.7%), Peptostreptococcus pevotii (4.7%), Prevotella buccae (4.7%), Prevotella intermedia (4.7%), Actinomyces meyeri (4.7%), Actinomyces viscosus (4.7%), Propionibacterium propionicum (4.7%), Bacteroides capillosus (4.7%), Staphylococcus hominis (4.7%), Rothia denticariosa (4.7%), Gemella haemolysans (4.7%), and Fusobacterium nucleatum (4.7%).ConclusionsResults of this study demonstrated that radicular cysts show a great variety of anaerobic and facultative anaerobic bacterial flora. It was observed that all isolated microorganisms were the types commonly found in oral flora. Although no specific microorganism was found, Streptococcus spp. bacteria (47.5%) – especially SMG (23.8%) – were predominantly found in the microorganisms isolated. Furthermore, radicular cysts might be polymicrobial originated. Although radicular cyst is an inflammatory cyst, some radicular cyst fluids might be sterile.

Bacterial genera in the fluids from apical periodontitis‐related radicular cysts: An observational study

“…Cystic fluids from RCs showed a remarkably reduced bacterial alpha-diversity, consisting mainly of anaerobic gram-negative bacteria in line with other studies (Iatrou et al, 1988;Scalas et al, 2013;Tek et al, 2013). Our results are consistent with previously reported NGS findings (Subramanian & Mickel, 2009), where a decrease in bacterial diversity and abundance of bacterial content in the periapex compared to the root ends was observed.…”

“…Cutibacterium acnes ( Propionibacterium acnes ) and Arachnia propionica ( Propionibacterium propionicum, Pseudopropionibacterium propionicum ) were found in the cystic fluids in previous studies (Scalas et al., 2013 ; Tek et al., 2013 ) and/or generally in the periapex (Mussano et al., 2018 ; Signoretti et al., 2013 ). The genus Pseudopropionibacterium , to which both bacteria previously belonged, was detected only in a few samples of cryopulverized teeth and cystic fluids but was almost entirely missing in supragingival plaques in our study.…”

“…Streptococcus sp., Fusobacterium nucleatum and Prevotella intermedia were previously found to be the predominant bacteria cultured from RCs (Tek et al., 2013 ). Our findings are in line with this observation; however, due to the NGS approach, we also found high relative abundances of other anaerobic gram‐negative bacterial genera (such as Porphyromonas and Fretibacterium ) in the cystic fluids.…”

Bacterial genera in the fluids from apical periodontitis‐related radicular cysts: An observational study

“…Cystic fluids from RCs showed a remarkably reduced bacterial alpha-diversity, consisting mainly of anaerobic gram-negative bacteria in line with other studies (Iatrou et al, 1988;Scalas et al, 2013;Tek et al, 2013). Our results are consistent with previously reported NGS findings (Subramanian & Mickel, 2009), where a decrease in bacterial diversity and abundance of bacterial content in the periapex compared to the root ends was observed.…”

“…Cutibacterium acnes ( Propionibacterium acnes ) and Arachnia propionica ( Propionibacterium propionicum, Pseudopropionibacterium propionicum ) were found in the cystic fluids in previous studies (Scalas et al., 2013 ; Tek et al., 2013 ) and/or generally in the periapex (Mussano et al., 2018 ; Signoretti et al., 2013 ). The genus Pseudopropionibacterium , to which both bacteria previously belonged, was detected only in a few samples of cryopulverized teeth and cystic fluids but was almost entirely missing in supragingival plaques in our study.…”

“…Streptococcus sp., Fusobacterium nucleatum and Prevotella intermedia were previously found to be the predominant bacteria cultured from RCs (Tek et al., 2013 ). Our findings are in line with this observation; however, due to the NGS approach, we also found high relative abundances of other anaerobic gram‐negative bacterial genera (such as Porphyromonas and Fretibacterium ) in the cystic fluids.…”

Apical periodontitis: preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing

“…It may be noteworthy that the anaerobic taxa detected were most abundant in RCs samples. F. nucleatum , P. intermedia and R. dentocariosa were observed to be statistically more abundant in those samples and, as previously reported, the presence of these taxa suggested the onset of secondary infection [ 27 ]. In addition, Iatrou et al [ 31 ] determined in their study that the isolated bacteria were mostly anaerobic.…”

Integrated metagenomics and transcriptomics analysis reveals pathways associated with oral periapical lesions formation and progression

“…Although sequencing methods are widely used, no study has compared the microbial profiles of periapical lesions and healthy pulp tissues, nor explored their correlation with the human transcriptome. Current findings show that radicular cysts exhibit diverse bacterial profiles ( Tek et al, 2013 ), while healthy pulp displays a distinct ( Widmer et al, 2018 ) and significantly higher microbial diversity than periapical abscesses, likely due to neutrophil activity and bacterial DNA degradation in abscesses ( Arnett et al, 2014 ; Ha et al, 2020 ).…”

Bacterial genera in the fluids from apical periodontitis‐related radicular cysts: An observational study

“…Cystic fluids from RCs showed a remarkably reduced bacterial alpha-diversity, consisting mainly of anaerobic gram-negative bacteria in line with other studies (Iatrou et al, 1988;Scalas et al, 2013;Tek et al, 2013). Our results are consistent with previously reported NGS findings (Subramanian & Mickel, 2009), where a decrease in bacterial diversity and abundance of bacterial content in the periapex compared to the root ends was observed.…”

“…Cutibacterium acnes ( Propionibacterium acnes ) and Arachnia propionica ( Propionibacterium propionicum, Pseudopropionibacterium propionicum ) were found in the cystic fluids in previous studies (Scalas et al., 2013 ; Tek et al., 2013 ) and/or generally in the periapex (Mussano et al., 2018 ; Signoretti et al., 2013 ). The genus Pseudopropionibacterium , to which both bacteria previously belonged, was detected only in a few samples of cryopulverized teeth and cystic fluids but was almost entirely missing in supragingival plaques in our study.…”

“…Streptococcus sp., Fusobacterium nucleatum and Prevotella intermedia were previously found to be the predominant bacteria cultured from RCs (Tek et al., 2013 ). Our findings are in line with this observation; however, due to the NGS approach, we also found high relative abundances of other anaerobic gram‐negative bacterial genera (such as Porphyromonas and Fretibacterium ) in the cystic fluids.…”

Apical periodontitis: preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing

“…It may be noteworthy that the anaerobic taxa detected were most abundant in RCs samples. F. nucleatum , P. intermedia and R. dentocariosa were observed to be statistically more abundant in those samples and, as previously reported, the presence of these taxa suggested the onset of secondary infection [ 27 ]. In addition, Iatrou et al [ 31 ] determined in their study that the isolated bacteria were mostly anaerobic.…”

Integrated metagenomics and transcriptomics analysis reveals pathways associated with oral periapical lesions formation and progression

“…Although sequencing methods are widely used, no study has compared the microbial profiles of periapical lesions and healthy pulp tissues, nor explored their correlation with the human transcriptome. Current findings show that radicular cysts exhibit diverse bacterial profiles ( Tek et al, 2013 ), while healthy pulp displays a distinct ( Widmer et al, 2018 ) and significantly higher microbial diversity than periapical abscesses, likely due to neutrophil activity and bacterial DNA degradation in abscesses ( Arnett et al, 2014 ; Ha et al, 2020 ).…”

Bacterial genera in the fluids from apical periodontitis‐related radicular cysts: An observational study

“…Cystic fluids from RCs showed a remarkably reduced bacterial alpha-diversity, consisting mainly of anaerobic gram-negative bacteria in line with other studies (Iatrou et al, 1988;Scalas et al, 2013;Tek et al, 2013). Our results are consistent with previously reported NGS findings (Subramanian & Mickel, 2009), where a decrease in bacterial diversity and abundance of bacterial content in the periapex compared to the root ends was observed.…”

“…Cutibacterium acnes ( Propionibacterium acnes ) and Arachnia propionica ( Propionibacterium propionicum, Pseudopropionibacterium propionicum ) were found in the cystic fluids in previous studies (Scalas et al., 2013 ; Tek et al., 2013 ) and/or generally in the periapex (Mussano et al., 2018 ; Signoretti et al., 2013 ). The genus Pseudopropionibacterium , to which both bacteria previously belonged, was detected only in a few samples of cryopulverized teeth and cystic fluids but was almost entirely missing in supragingival plaques in our study.…”

“…Streptococcus sp., Fusobacterium nucleatum and Prevotella intermedia were previously found to be the predominant bacteria cultured from RCs (Tek et al., 2013 ). Our findings are in line with this observation; however, due to the NGS approach, we also found high relative abundances of other anaerobic gram‐negative bacterial genera (such as Porphyromonas and Fretibacterium ) in the cystic fluids.…”

Apical periodontitis: preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing

“…It may be noteworthy that the anaerobic taxa detected were most abundant in RCs samples. F. nucleatum , P. intermedia and R. dentocariosa were observed to be statistically more abundant in those samples and, as previously reported, the presence of these taxa suggested the onset of secondary infection [ 27 ]. In addition, Iatrou et al [ 31 ] determined in their study that the isolated bacteria were mostly anaerobic.…”

Integrated metagenomics and transcriptomics analysis reveals pathways associated with oral periapical lesions formation and progression

“…Although sequencing methods are widely used, no study has compared the microbial profiles of periapical lesions and healthy pulp tissues, nor explored their correlation with the human transcriptome. Current findings show that radicular cysts exhibit diverse bacterial profiles ( Tek et al, 2013 ), while healthy pulp displays a distinct ( Widmer et al, 2018 ) and significantly higher microbial diversity than periapical abscesses, likely due to neutrophil activity and bacterial DNA degradation in abscesses ( Arnett et al, 2014 ; Ha et al, 2020 ).…”