The potential of Raman spectroscopy for the identification of biofilm formation by<i>Staphylococcus epidermidis</i>

Samek, Ota; Almarashi, Jamal F. M.; Telle, Helmut H.

doi:10.1002/lapl.200910154

Cited by 66 publications

(53 citation statements)

References 33 publications

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“…Protein (BSA) solutions were used as standards to compare relative hydration. In this context, Samek et al acquired Raman spectra of the biofilm-forming Staphylococcus epidermidis 1457 strain and its non-biofilm-forming mutant Staphylococcus epidermidis 1457-M10 strain after growth of each on Columbia blood agar culture plates for 24 h [32]. Although there were subtle differences between the spectra of the two strains, the principal component analysis (PCA) of the spectra clearly discriminated the two strains and showed the effects of the biofilm matrix ingredients on the spectra, especially that of polysaccharide inter-cellular adhesions (PIA).…”

Section: Raman Spectroscopy Of Biofilmsmentioning

confidence: 99%

Raman and Surface-Enhanced Raman Scattering for Biofilm Characterization

2018

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Biofilms are a communal way of living for microorganisms in which microorganism cells are surrounded by extracellular polymeric substances (EPS). Most microorganisms can live in biofilm form. Since microorganisms are everywhere, understanding biofilm structure and composition is crucial for making the world a better place to live, not only for humans but also for other living creatures. Raman spectroscopy is a nondestructive technique and provides fingerprint information about an analyte of interest. Surface-enhanced Raman spectroscopy is a form of this technique and provides enhanced scattering of the analyte that is in close vicinity of a nanostructured noble metal surface such as silver or gold. In this review, the applications of both techniques and their combination with other biofilm analysis techniques for characterization of composition and structure of biofilms are discussed.

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Section: Raman Spectroscopy Of Biofilmsmentioning

confidence: 99%

Raman and Surface-Enhanced Raman Scattering for Biofilm Characterization

2018

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“…The investigation of microorganisms [13] as well as the examination of biofilm formations [1,[14][15][16] by using Raman microspectroscopy has currently become quite popular. Components such as DNA, RNA, proteins, lipids, and carbohydrates of the bacterial cells may vary and, therefore, cause the Raman spectra of two different species to be slightly different which can be successfully used for species discrimination [17,18].…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopic differentiation of planktonic bacteria and biofilms

et al. 2015

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Both biofilm formations as well as planktonic cells of water bacteria such as diverse species of the Legionella genus as well as Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli were examined in detail by Raman microspectroscopy. Production of various molecules involved in biofilm formation of tested species in nutrient-deficient media such as tap water was observed and was particularly evident in the biofilms formed by six Legionella species. Biofilms of selected species of the Legionella genus differ significantly from the planktonic cells of the same organisms in their lipid amount. Also, all Legionella species have formed biofilms that differ significantly from the biofilms of the other tested genera in the amount of lipids they produced. We believe that the significant increase in the synthesis of this molecular species may be associated with the ability of Legionella species to form biofilms. In addition, a combination of Raman microspectroscopy with chemometric approaches can distinguish between both planktonic form and biofilms of diverse bacteria and could be used to identify samples which were unknown to the identification model. Our results provide valuable data for the development of fast and reliable analytic methods based on Raman microspectroscopy, which can be applied to the analysis of tap water-adapted microorganisms without any cultivation step.

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“…In order to extract quantitative information from the experimentally obtained spectral data, we adopted the Rolling Circle Filter (RCF) technique for background removal [1][2][3]. In principle, RCF is a high-pass signal filter that allows separating the narrow Raman spectral peaks from the background whose radius of curvature is significantly higher.…”

Section: Methodsmentioning

confidence: 99%

“…In many applications optical trapping has been combined with Raman microspectroscopy which provides non-invasive spatially resolved mapping of molecular composition of studied sample. Recently, it has been shown that the technique of Raman spectroscopy (including Raman imaging) can be regarded as the method of choice for many studies of microorganisms, cells and biological samples [1][2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopy for bacterial identification and characterization

et al. 2012

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The main goal of our investigation is to use Raman tweezers technique so that the responce of Raman scattering on microorganisms suspended in liquid media (bacteria, algae and yeast cells in microfluidic chips) can be used to identify different species. The investigations presented here include identification of different bacteria strains (biofilm-positive and biofilm-negative) and yeast cells by using principal component analysis (PCA). The main driving force behind our investigation was a common problem in the clinical microbiology laboratory -how to distinguish between contaminant and invasive isolates. Invasive bacterial/yeast isolates can be assumed to form a biofilm, while isolates which do not form a biofilm can be treated as contaminant. Thus, the latter do not represent an important virulence factor.

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The potential of Raman spectroscopy for the identification of biofilm formation byStaphylococcus epidermidis

Cited by 66 publications

References 33 publications

Raman and Surface-Enhanced Raman Scattering for Biofilm Characterization

Raman and Surface-Enhanced Raman Scattering for Biofilm Characterization

Raman spectroscopic differentiation of planktonic bacteria and biofilms

Raman spectroscopy for bacterial identification and characterization

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