The potential of CD44 as a diagnostic and prognostic tool in oral cancer

Emich, Helena; Chapireau, David; Hutchison, Iain; Mackenzie, Ian C.

doi:10.1111/jop.12308

Cited by 22 publications

(27 citation statements)

References 82 publications

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“…CD44s play an important role as tumor-promoting oncoproteins in early localized ampullary adenocarcinoma, while CD44 variants were correlated with advanced cancer and recurrent disease [4]. Recent studies have suggested that CD44 is probably a type of stem cell marker and useful as a diagnostic and prognostic tool in many cancers, including non-small cell lung cancer (NSCLC), breast cancer, gastric cancer, and oral cancer [2,5,6].…”

Section: Introductionmentioning

confidence: 99%

Expression of Novel CD44st and MMP2 in NSCLC Tissues and Their Clinical Significance

Zhang

Hai

et al. 2017

Oncol Res Treat

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Background: Abnormal expression of some CD44 molecules in tumor tissues can induce the degradation of the extracellular matrix, and is closely associated with axillary lymph node metastasis, angiogenesis, cancer progression, and drug resistance. Patients and Methods: We measured and confirmed the expression of CD44st and MMP2 mRNAs and proteins in the cancer tissues and adjacent normal tissues of postoperative non-small cell lung cancer (NSCLC) patients with either adenocarcinoma (n = 72) or squamous cell carcinoma (n = 53) using quantitative reverse transcription polymerase chain reaction, gene sequencing, and immunohistochemical analysis. Results: CD44st and MMP2 expression were closely associated with the histopathological classification, lymph node metastasis, and TNM stage of the tumors, and the difference was statistically significant (p < 0.05). The median overall survival (OS) for the high CD44st expression group was 30.52 months (95% confidence interval (CI) 24.02-36.15); for the low expression group it was 43.23 months (95% CI 31.81-52.02) (p = 0.020). The median OS for the high MMP-2 expression group was 30.53 months (95% CI 26.69-33.31); for the low expression group it was 40.06 months (95% CI 33.55-46.45) (p = 0.022). Conclusion: The rates of CD44st and MMP2 expression were higher in squamous cell carcinomas than in adenocarcinomas, were closely associated with lymph node metastasis and TNM stage, and affected patients' prognoses.

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Section: Introductionmentioning

confidence: 99%

Expression of Novel CD44st and MMP2 in NSCLC Tissues and Their Clinical Significance

Zhang

Hai

et al. 2017

Oncol Res Treat

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“…Since it has been recognized that CSCs drives tumor progression and resistance to therapy, different stem cell markers have been investigated in solid tumors. An association among clinical pathological parameters, aggressiveness, and prognosis is expected in tumors with high expression of different stem cell markers, including CD44, NANOG, OCT4, and BMI1 . In this article, we have showed that there is a significant difference in the expression of CD44s , CD44v3 , NANOG , OCT4 , and BMI1 in tongue SCC in relation with nontumoral margins.…”

Section: Discussionmentioning

confidence: 65%

“…An association among clinical pathological parameters, aggressiveness, and prognosis is expected in tumors with high expression of different stem cell markers, including CD44, NANOG, OCT4, and BMI1. 3,23,24 In this article, we have showed that there is a significant difference in the expression of CD44s, CD44v3, NANOG, OCT4, and BMI1 in tongue SCC in relation with nontumoral margins. Additionally, CD44, NANOG, and OCT4 were positively associated with poor prognosis in patients with tongue SCC.…”

Section: Discussionmentioning

confidence: 80%

“…Many IHC studies have also investigated the relationship between CD44 expression and the relevant clinical aspects of OSCC; however, the published data are largely controversial. 24,33 Although some studies have reported the association of high CD44 expression with advanced disease, metastasis, invasion, and worse overall survival, 34 others have shown low CD44 expression to be indicative of more aggressive and poorly differentiated tumors 35,36 or have shown the lack of a correlation between TNM classification and survival. 37 In a systematic review and meta-analysis study evaluating the prognosis significance of CD44 expression in oral and pharyngeal cancer, Chen et al 38 surprisingly found that CD44 expression predicts an improved disease-free survival rate, although no association with clinicopathological features was observed.…”

Section: Discussionmentioning

confidence: 99%

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Prognostic implications of CD44, NANOG, OCT4, and BMI1 expression in tongue squamous cell carcinoma

et al. 2018

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“…Ela medeia interações célula-célula e célulamatriz em grande parte por conta de sua afinidade com o ácido hialurônico, que é um constituinte da matriz extracelular. A CD44 também apresenta afinidade com outros ligantes, como a osteopontina e metaloproteinases da matriz (MMPs)(Cichy;Puré, 2003;Zhang et al, 2012;Emich et al, 2015).Pesquisas já mostraram a associação da expressão de CD44 com vias de sinalização que influenciam o crescimento, propagação e resistência do CEO(Al-Hajj et al, 2003;Prince et al, 2007;Zhang et al, 2012;Emich et al, 2015). Isso ocorre devido à interação da CD44 e do ácido hialurônico no domínio extracelular, promovendo vias de sinalização que desempenham papeis importantes na progressão tumoral de diversos tipos de câncer(Thapa;Wilson, 2016).Essa proteína já foi usada no isolamento e na caracterização das células tronco tumorais em carcinomas epidermóides de cabeça e pescoço.Prince et.al.…”

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Análise do fenótipo de células-tronco neoplásicas em células epiteliais displásicas orais e de carcinoma epidermóide oral resistentes à terapia fotodinâmica

Buck¹,

Corrêa²

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PDT de ambas as linhagens exibiram superexpressão de CD44 high /ESA high . A linhagem SCC9 exibiu ainda maior frequência de células CD44 high /ESA low e CD44 high /p75 NTR+ , sugerindo a presença de uma subpopulação com alto potencial de invasão e resistente aos tratamentos convencionais. Não houve diferenças significativas na frequência de células ESA high /p75 NTR + em ambas as linhagens.Concluiu-se que as células displásicas orais desenvolvem resistência à PDT com intensidade menor se comparada às células SCC9. Em ambas as linhagens, as células resistentes exibiram um fenótipo sugestivo de células tronco tumorais. Esse fato deve ser considerado ao se instituir a PDT mediada pelo 5-ALA em lesões displásicas e neoplásicas orais que demandam múltiplos ciclos de tratamento. Palavras-chave: Terapia fotodinâmica. Ácido 5-aminolevulínico. Lesões potencialmente malignas orais. Carcinoma epidermóide oral. Resistência tumoral. ABSTRACT Buck MGT. Analysis of the tumor stem cell phenotype in oral dysplastic epithelial cells and oral squamous cell carcinoma resistant to photodynamic therapy [thesis]. São Paulo: Universidade de São Paulo, Faculdade de Odontologia; 2018. Versão Corrigida.Oral squamous cell carcinoma (SCC) has a high incidence and mortality in the world.There are frequent cases of relapses due to its resistance to conventional treatments.This resistance has been associated with the presence of cancer stem cells (CSC).Photodynamic therapy (PDT) has been used in cases of potentially malignant oral lesions (PMOL) and superficial SCC's, with a relative success rate. However, relapses are reported, which suggest some resistance to PDT. The aim of the present study was to evaluate whether PMOL cells develop resistance to PDT mediated by the 5aminolevulinic acid (5-ALA), as occurs with SCC cells exposed to the same treatment, as well as to verify if dysplastic and oral neoplastic cells exhibit a suggestive phenotype of CSC. Oral dysplastic epithelial cells (DOK lineage) and CSC cells of the tongue (SCC9 lineage) were divided into the following experimental groups: Control Groupcells with no treatment; Group ALA -5-ALA treated cells, with concentration and incubation time equal to that of the PDT group; LED group -LED light treated cells, with dosimetry equal to that of the PDT group; PDT group -cells treated with PDT mediated by the 5-ALA, subdivided into two generations: Generation 1 -cells exposed to only one PDT cycle; Generation 4 -cells exposed to four PDT cycles and exhibiting resistance (greater viability) to the initial dose applied for Generation 1. Colony forming and invasion assays, as well as flow cytometry to verify the expression of CD44, ESA and p75 NTR were performed to the experimental groups. It was observed that all groups exposed to PDT containing DOK lineage cells exhibited resistance to the initial dose of PDT, but at a lower intensity than those observed for the SCC9 lineage. PDTresistant cells from both lineages exhibited increased potential for colony formation, but only the first generation...

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The potential of CD44 as a diagnostic and prognostic tool in oral cancer

Cited by 22 publications

References 82 publications

Expression of Novel CD44st and MMP2 in NSCLC Tissues and Their Clinical Significance

Expression of Novel CD44st and MMP2 in NSCLC Tissues and Their Clinical Significance

Prognostic implications of CD44, NANOG, OCT4, and BMI1 expression in tongue squamous cell carcinoma

Análise do fenótipo de células-tronco neoplásicas em células epiteliais displásicas orais e de carcinoma epidermóide oral resistentes à terapia fotodinâmica

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