The potential importance of methylated substrates in methane production within three northern Minnesota peatlands

Hanna, Emily; Keller, Jason K.; Chang, Daniel K.; Bruyn, Warren de; Zalman, Cassandra

doi:10.1016/j.soilbio.2020.107957

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…These MAGs exhibited strong depth stratification, indicating that members of the Methanobacteriaceae may be more important methylotrophs in the surface (10 to 20 cm), whereas C. methanomethyliaceae become dominant in deeper peat (100 to 175 cm). Typically, it has been assumed that the majority of CH 4 in peatlands is produced via the hydrogenotrophic and acetoclastic pathways and only recently has the potential importance of methylotrophic methanogenesis gained attention (61,74,75). Further, methanol is a degradation product of lignin, and the accumulation of lignin-like compounds in the DOM (SI Appendix, Fig.…”

Section: Discussionmentioning

confidence: 99%

Soil metabolome response to whole-ecosystem warming at the Spruce and Peatland Responses under Changing Environments experiment

Wilson

Tfaily

Kolton

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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In this study, a suite of complementary environmental geochemical analyses, including NMR and gas chromatography-mass spectrometry (GC-MS) analyses of central metabolites, Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) of secondary metabolites, and lipidomics, was used to investigate the influence of organic matter (OM) quality on the heterotrophic microbial mechanisms controlling peatland CO2, CH4, and CO2:CH4 porewater production ratios in response to climate warming. Our investigations leverage the Spruce and Peatland Responses under Changing Environments (SPRUCE) experiment, where air and peat warming were combined in a whole-ecosystem warming treatment. We hypothesized that warming would enhance the production of plant-derived metabolites, resulting in increased labile OM inputs to the surface peat, thereby enhancing microbial activity and greenhouse gas production. Because shallow peat is most susceptible to enhanced warming, increases in labile OM inputs to the surface, in particular, are likely to result in significant changes to CO2 and CH4 dynamics and methanogenic pathways. In support of this hypothesis, significant correlations were observed between metabolites and temperature consistent with increased availability of labile substrates, which may stimulate more rapid turnover of microbial proteins. An increase in the abundance of methanogenic genes in response to the increase in the abundance of labile substrates was accompanied by a shift toward acetoclastic and methylotrophic methanogenesis. Our results suggest that as peatland vegetation trends toward increasing vascular plant cover with warming, we can expect a concomitant shift toward increasingly methanogenic conditions and amplified climate–peatland feedbacks.

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Section: Discussionmentioning

confidence: 99%

Soil metabolome response to whole-ecosystem warming at the Spruce and Peatland Responses under Changing Environments experiment

Wilson

Tfaily

Kolton

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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“…A Bray-Curtis index calculated between these 22 samples revealed that the microbial community compositions were mainly structured according to sample type and by sampling site (see network topology in Figure 1 ; Table 1 ). So far, some of the anoxic habitats where Methanomassiliicoccales have been detected were reported to contain fermentation products and methylated compounds [ 67 , 68 , 69 , 70 , 71 ]. The methylated compounds result from the anaerobic degradation of organic compounds, such as pectin (for methanol) or N -methylated compounds (e.g., choline, betaine or trimethylamine- N -oxide), which are particularly represented in marine habitats [ 72 ], where they mainly serve as osmoregulators in organisms.…”

Section: Resultsmentioning

confidence: 99%

New Insights into the Ecology and Physiology of Methanomassiliicoccales from Terrestrial and Aquatic Environments

et al. 2020

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Members of the archaeal order Methanomassiliicoccales are methanogens mainly associated with animal digestive tracts. However, environmental members remain poorly characterized as no representatives not associated with a host have been cultivated so far. In this study, metabarcoding screening combined with quantitative PCR analyses on a collection of diverse non-host-associated environmental samples revealed that Methanomassiliicoccales were very scarce in most terrestrial and aquatic ecosystems. Relative abundance of Methanomassiliicoccales and substrates/products of methanogenesis were monitored during incubation of environmental slurries. A sediment slurry enriched in Methanomassiliicoccales was obtained from a freshwater sample. It allowed the reconstruction of a high-quality metagenome-assembled genome (MAG) corresponding to a new candidate species, for which we propose the name of Candidatus ‘Methanomassiliicoccus armoricus MXMAG1’. Comparison of the annotated genome of MXMAG1 with the published genomes and MAGs from Methanomassiliicoccales belonging to the 2 known clades (‘free-living’/non-host-associated environmental clade and ‘host-associated’/digestive clade) allowed us to explore the putative physiological traits of Candidatus ‘M. armoricus MXMAG1’. As expected, Ca. ‘Methanomassiliicoccus armoricus MXMAG1’ had the genetic potential to produce methane by reduction of methyl compounds and dihydrogen oxidation. This MAG encodes for several putative physiological and stress response adaptations, including biosynthesis of trehalose (osmotic and temperature regulations), agmatine production (pH regulation), and arsenic detoxication, by reduction and excretion of arsenite, a mechanism that was only present in the ‘free-living’ clade. An analysis of co-occurrence networks carried out on environmental samples and slurries also showed that Methanomassiliicoccales detected in terrestrial and aquatic ecosystems were strongly associated with acetate and dihydrogen producing bacteria commonly found in digestive habitats and which have been reported to form syntrophic relationships with methanogens.

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Microbial communities and functions are structured by vertical geochemical zones in a northern peatland

Wang,

Yu,

Zhou

et al. 2024

Science of The Total Environment

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The potential importance of methylated substrates in methane production within three northern Minnesota peatlands

Cited by 3 publications

References 27 publications

Soil metabolome response to whole-ecosystem warming at the Spruce and Peatland Responses under Changing Environments experiment

Soil metabolome response to whole-ecosystem warming at the Spruce and Peatland Responses under Changing Environments experiment

New Insights into the Ecology and Physiology of Methanomassiliicoccales from Terrestrial and Aquatic Environments

Microbial communities and functions are structured by vertical geochemical zones in a northern peatland

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