The potential for clinical applications using a new ionization method combined with ion mobility spectrometry-mass spectrometry

Inutan, Ellen D.; Wager‐Miller, James; Narayan, Srinivas B.; Trimpin, Sarah

doi:10.1007/s12127-013-0131-7

Cited by 33 publications

(109 citation statements)

References 109 publications

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“…The MH + and MH 2 2+ ions, m/z 336 and m/z 168, respectively, appear well above background. As previously reported, the MAI method also works well using salty and buffered solutions [23,25,26]. This is demonstrated with Method 3 using a urine sample spiked with LSD to make a 1-ppm solution.…”

Section: Resultssupporting

confidence: 65%

High Sensitivity Analysis of Nanoliter Volumes of Volatile and Nonvolatile Compounds using Matrix Assisted Ionization (MAI) Mass Spectrometry

Hoang

Pophristić

Horan

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. First results are reported using a simple, fast, and reproducible matrixassisted ionization (MAI) sample introduction method that provides substantial improvements relative to previously published MAI methods. The sensitivity of the new MAI methods, which requires no laser, high voltage, or nebulizing gas, is comparable to those reported for MALDI-TOF and n-ESI. High resolution full acquisition mass spectra having low chemical background are acquired from low nanoliters of solution using only a few femtomoles of analyte. The limit-of-detection for angiotensin II is less than 50 amol on an Orbitrap Exactive mass spectrometer. Analysis of peptides, including a bovine serum albumin digest, and drugs, including drugs in urine without a purification step, are reported using a 1 μL zero dead volume syringe in which only the analyte solution wetting the walls of the syringe needle is used in the analysis.

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Section: Resultssupporting

confidence: 65%

High Sensitivity Analysis of Nanoliter Volumes of Volatile and Nonvolatile Compounds using Matrix Assisted Ionization (MAI) Mass Spectrometry

Hoang

Pophristić

Horan

et al. 2016

J. Am. Soc. Mass Spectrom.

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“…Analytes of interest can be quantified in lower ppm levels using MAI-MS and MS/MS directly from urine without dilution or sample treatment. With further engineering and method development, it seems reasonable that increased reproducibility, sensitivity, and speed of analyses will be obtainable, making MAI increasingly viable 22,27 for use in clinical laboratories. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 Figure S12) for the characterization of hydrocodone.…”

Section: Resultsmentioning

confidence: 99%

“…21 MAI is applicable to a variety of commercial mass spectrometers 123,8,21-25 eliminating the need of an ion source, and producing mass spectra from complex samples such as blood, urine, and tissue. 2,26,27 In addition, MAI has recently been shown to be capable of high-throughput and automated analysis, 26 requiring only a few seconds per sample. MAI is hypothesized to spontaneously produce gas-phase ions from a solid matrix by a sublimation driven triboluminescence process when the matrix:analyte sample is exposed to vacuum.…”

Section: Mass Spectrometry (Ms) Is a Powerful Newmentioning

confidence: 99%

Reproducibility and Quantification of Illicit Drugs Using Matrix-Assisted Ionization (MAI) Mass Spectrometry

Chakrabarty¹,

DeLeeuw

Woodall

et al. 2015

Anal. Chem.

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Matrix-assisted ionization (MAI) mass spectrometry (MS) is a simple and sensitive method for analysis of low- and high-mass compounds, requiring only that the analyte in a suitable matrix be exposed to the inlet aperture of an atmospheric pressure ionization mass spectrometer. Here, we evaluate the reproducibility of MAI and its potential for quantification using six drug standards. Factors influencing reproducibility include the matrix compound used, temperature, and the method of sample introduction. The relative standard deviation (RSD) using MAI for a mixture of morphine, codeine, oxymorphone, oxycodone, clozapine, and buspirone and their deuterated internal standards using the matrix 3-nitrobenzonitrile is less than 10% with either a Waters SYNAPT G2 or a Thermo LTQ Velos mass spectrometer. The RSD values obtained using MAI are comparable to those using ESI or MALDI on these instruments. The day-to-day reproducibility of MAI determined for five consecutive days with internal standards was better than 20% using manual sample introduction. The reproducibility improved to better than 5% using a mechanically assisted sample introduction method. Hydrocodone, present in a sample of undiluted infant urine, was quantified with MAI using the standard addition method.

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“…Unlike secondary ion mass spectrometry, MALDI, ESI, DESI, ASAP, DART, LSI, SAII, or MAII, no high‐energy source (voltage, laser, ion beam, or heat) is required to achieve ionization by this novel method. This matrix assisted ionization vacuum (MAIV) method is assisted by the vacuum of a mass spectrometer and operates by placing the matrix:analyte sample in vacuum, similar to vacuum MALDI, or from atmospheric pressure sources without the necessity for an air‐tight closure …”

mentioning

confidence: 99%

“…In addition to the numerous ambient ionization methods developed thus far, these novel inlet and vacuum ionization methods have been shown to be useful in biological materials characterization and are of fundamental interest . Previous interrogations have shown that 1 M sodium chloride solutions produce mainly protonated ions from drugs and proteins, indicating that metal cation attachment is not a favorable process using MAIV. Cationization, however, is essential for polymer ionization .…”

mentioning

confidence: 99%

Characterizing synthetic polymers and additives using new ionization methods for mass spectrometry

El‐Baba

Lutomski

Wang

et al. 2014

Rapid Comm Mass Spectrometry

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The potential for clinical applications using a new ionization method combined with ion mobility spectrometry-mass spectrometry

Cited by 33 publications

References 109 publications

High Sensitivity Analysis of Nanoliter Volumes of Volatile and Nonvolatile Compounds using Matrix Assisted Ionization (MAI) Mass Spectrometry

High Sensitivity Analysis of Nanoliter Volumes of Volatile and Nonvolatile Compounds using Matrix Assisted Ionization (MAI) Mass Spectrometry

Reproducibility and Quantification of Illicit Drugs Using Matrix-Assisted Ionization (MAI) Mass Spectrometry

Characterizing synthetic polymers and additives using new ionization methods for mass spectrometry

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