The potential activity of biosynthesized silver nanoparticles of Pseudomonas aeruginosa as an antibacterial agent against multidrug-resistant isolates from intensive care unit and anticancer agent

Abstract

Background Antibiotic resistance is a global problem; especially the multidrug-resistant bacteria are a serious and fatal problem in the intensive care unit. Interestingly, biosynthesized silver nanoparticles are the promising key to eliminate these microbes. Using Pseudomonas aeruginosa supernatant is an easy and cheap method in silver nanoparticle biosynthesis. The biosynthesis conditions were adjusted, and the profiling of the biosynthesized silver nanoparticles was confirmed. Results The UV spectroscopy at a wavelength at 400 nm was 0.539 A.U., transmission electron microscope showed nanoparticles were homogeneous with a square and spherical shape, its average size 20 nm, The capping material and the existence of silver nanoparticles were confirmed using Fourier transform infrared spectroscopy, and energy dispersive X-ray spectroscopy. The minimum inhibitory concentration was 1 mg/ml against multidrug-resistant bacteria, and LC50 was 62.307 μg/ml on the hepatocellular carcinoma cell line. Conclusions Microbial-synthesized silver nanoparticles have a potential application to combat multidrug-resistant bacteria, especially in the intensive care unit.

Biosynthesis of silver nanoparticles using Pseudomonas canadensis, and its antivirulence effects against Pseudomonas tolaasii, mushroom brown blotch agent

“…The biosynthesized AgNPs showed a peak at 425 nm in UV/Visible spectral analysis. This result is in agreement with previous studies that confirmed the formation of AgNPs by other bacteria with the presence of the peak at 400–450 nm 35 . Analysis through an XRD spectrometer confirmed the presence of an elemental silver signal of the AgNPs.…”

“…There has been no report of the use of bacteria closely related to P. canadensis for the synthesis of AgNPs. Although, other taxonomically related strains like P. hibiscicola , P. aeruginosa , and P. stutzeri have been reported for the synthesis of silver nanoparticles 35 – 37 .…”

Design rules applied to silver nanoparticles synthesis: A practical example of machine learning application.

“…Already available on the market, silver nanoparticles (AgNPs) embedded into products offer a variety of benefits such as an antimicrobial functionalities by eradicating or inhibiting the growth of bacteria [1] , fungi [2] , and viruses [3] , as well as the ability to prolong the shelf life of products (i.e., food packaging). Their versatility renders them valuable in various products’ applications including wound dressings [4] , medical devices [5] , [6] , water purification systems [7] , and antibacterial coatings [8] .…”

Molecular Analysis of Carbapenem and Aminoglycoside Resistance Genes in Carbapenem-Resistant Pseudomonas aeruginosa Clinical Strains: A Challenge for Tertiary Care Hospitals

“…One of the possible strategy to manage resistance is to use combination treatment, especially with non-antibiotic drugs that may interact with antibiotics [55,56]. In this context, a prime non-antibiotic treatment is the use of silver nanoparticles (AgNPs) with powerful bactericidal properties against MDR microbes [57]. AgNPs facilitate antimicrobial uptake into bateria and lower the desired antibiotic dose required resulting in enhanced antimicrobial activity [58].…”