The possible mechanism of Hippophae fructus oil applied in tympanic membrane repair identified based on network pharmacology and molecular docking

Huang, Juntao; Teh, Bing Mei; Xu, Ziqian; Yuan, Zhechen; Zhou, Chongchang; Shi, Yunbin; Shen, Yi

doi:10.1002/jcla.24157

Cited by 5 publications

(1 citation statement)

References 56 publications

(106 reference statements)

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“…However, there is currently no research on Fructus hippophae genomic information. Studies on Fructus hippophae are currently limited to compounds and their related protein targets of Hippophae Fructus oil (HFO), relying on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP: https://old.tcmsp-e.com/tcmsp.php ) 22 . Other studies have focused on methods of extracting and purifying flavonoids, tannins, and other novel nutritional supplements from Fructus hippophae , which depend on spectrophotometry, chromatography and other chemical methods 23 – 25 .…”

Section: Background and Summarymentioning

confidence: 99%

Chromosome-level genome assembly of Hippophae rhamnoides variety

Yang,

Luo,

Yang

et al. 2024

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Fructus hippophae (Hippophae rhamnoides spp. mongolica×Hippophae rhamnoides sinensis), a hybrid variety of sea buckthorn that Hippophae rhamnoides spp. mongolica serves as the female parent and Hippophae rhamnoidessinensis serves as the male parent, is a traditional plant with great potentials of economic and medical values. Herein, we gained a chromosome-level genome of Fructus hippophae about 918.59 Mb, with the scaffolds N50 reaching 83.65 Mb. Then, we anchored 440 contigs with 97.17% of the total genome sequences onto 12 pseudochromosomes. Next, de-novo, homology and transcriptome assembly strategies were adopted for gene structure prediction. This predicted 36475 protein-coding genes, of which 36226 genes could be functionally annotated. Simultaneously, various strategies were used for quality assessment, both the complete BUSCO value (98.80%) and the mapping rate indicated the high assembly quality. Repetitive elements, which occupied 63.68% of the genome, and 1483600 bp of non-coding RNA were annotated. Here, we provide genomic information on female plants of a popular variety, which can provide data for pan-genomic construction of sea buckthorn and for the resolution of the mechanism of sex differentiation.

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Section: Background and Summarymentioning

confidence: 99%

Chromosome-level genome assembly of Hippophae rhamnoides variety

Yang,

Luo,

Yang

et al. 2024

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The possible mechanism of Hippophae fructus oil applied in tympanic membrane repair identified based on network pharmacology and molecular docking

Huang

Teh

et al. 2021

Clinical Laboratory Analysis

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Objective This study aimed to explore the mechanisms of Hippophae fructus oil (HFO) in the treatment of tympanic membrane (TM) perforation through network pharmacology‐based identification. Methods The compounds and related targets of HFO were extracted from the TCMSP database, and disease information was obtained from the OMIM, GeneCards, PharmGkb, TTD, and DrugBank databases. A Venn diagram was generated to show the common targets of HFO and TM, and GO and KEGG analyses were performed to explore the potential biological processes and signaling pathways. The PPI network and core gene subnetwork were constructed using the STRING database and Cytoscape software. A molecular docking analysis was also conducted to simulate the combination of compounds and gene proteins. Results A total of 33 compounds and their related targets were obtained from the TCMSP database. After screening the 393 TM‐related targets, 21 compounds and 22 gene proteins were selected to establish the network diagram. GO and KEGG enrichment analyses revealed that HFO may promote TM healing by influencing cellular oxidative stress and related signaling pathways. A critical subnetwork was obtained by analyzing the PPI network with nine core genes: CASP3, MMP2, IL1B, TP53, EGFR, CXCL8, ESR1, PTGS2, and IL6. In addition, a molecular docking analysis revealed that quercetin strongly binds the core proteins. Conclusion According to the analysis, HFO can be utilized to repair perforations by influencing cellular oxidative stress. Quercetin is one of the active compounds that potentially plays an important role in TM regeneration by influencing 17 gene proteins.

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Probing the mechanism of action (MOA) of Solanum nigrum Linn against breast cancer using network pharmacology and molecular docking

2023

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Solanum nigrum Linn is a medicinal herb widely used in traditional Chinese medicine to treat ailments such as fever, inflammation and cancer. Although quite a few compounds have been isolated and characterized, its anticancer mechanism remains elusive. Thus, in this study, we used network pharmacology and molecular docking strategies to identify the major active ingredients in S. nigrum and reveal its putative mechanism against human breast cancer. Six compounds, quercetin, cholesterol, 3-epi-beta-sitosterol, diosgenin, medioresinol and solanocapsine, were identified to be the major active ingredients. Target identification and analysis showed that they regulate 80 breast cancer-related targets. Furthermore, network analysis showed that the six active ingredients regulate multiple pathways including ErbB signaling pathway and estrogen signaling pathway and genes AKT1(AKT serine/threonine kinase 1), ESR1(estrogen receptor 1), EGFR(epidermal growth factor receptor), SRC(proto-oncogene tyrosine-protein kinase Src), AR(androgen receptor) and MMP9(matrix metalloproteinase 9) are crucial genes involved in the regulations. Molecular docking implied that quercetin could form good binding with AKT1, EGFR, SRC and MMP9. Our current study suggests that the anticancer function of S. nigrum is likely via synergistic/additive effects of multiple active ingredients’ regulations of different signaling pathways. Further studies are warranted to establish the standard for S. nuigrum to be used as a CAM (complementary and alternative medicine) in breast cancer treatment and dissect its potential interactions with chemotherapy drugs.

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The possible mechanism of Hippophae fructus oil applied in tympanic membrane repair identified based on network pharmacology and molecular docking

Cited by 5 publications

References 56 publications

Chromosome-level genome assembly of Hippophae rhamnoides variety

Chromosome-level genome assembly of Hippophae rhamnoides variety

The possible mechanism of Hippophae fructus oil applied in tympanic membrane repair identified based on network pharmacology and molecular docking

Probing the mechanism of action (MOA) of Solanum nigrum Linn against breast cancer using network pharmacology and molecular docking

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