The porcine follitropin receptor: cDNA cloning, functional expression and chromosomal localization of the gene

Rémy, Jean-Serge; Lahbib-Mansais, Y.; Yerle, Martine M.; Bozon, Véronique; Couture, Laurence; Pajot, Edith; Grebert, Denise; Salesse, Roland

doi:10.1016/0378-1119(95)00385-j

Cited by 27 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The porcine LH receptor (LHR) full-length cDNA cloned into the transcription vector pBluescript (Stratagene, La Jolla, CA) and the HindIII-BamHI 380 FSH receptor (FSHR) fragment contained within the extracellular domain [16] and subcloned in pGEM3z(Ϫ) vector (Promega, Madison, WI), were used as templates for the synthesis of antisense and sense RNA probes. The linearized plasmid (1 g) was incubated in the presence of 500 M ribonucleotide (r) ATP, rGTP, 50 Ci of [ 35 S]CTP, and 50 Ci of [ 35 S]UTP (Dupont de Nemours NEN, les Ulis, France) in the presence of T3 or T7 RNA polymerase (Promega) for 1 h at 37ЊC.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Comparative Expression of Luteinizing Hormone and Follicle-Stimulating Hormone Receptors in Ovarian Follicles from High and Low Prolific Sheep Breeds1

Abdennebi¹,

Monget²,

Pisselet³

et al. 1999

Biology of Reproduction

View full text Add to dashboard Cite

Expression of gonadotropin receptors and granulosa cell sensitivity to gonadotropin hormones by small (1-3 mm) and large (3.5-7 mm) follicles were compared in Romanov (ROM, ovulation rate = 3) and Ile-de-France (IF, ovulation rate = 1) ewes in the early and late follicular phase. In healthy follicles, LH receptor levels in granulosa cells increased with increasing follicular size (p < 0. 001) while FSH receptor levels decreased (p < 0.05). In granulosa cells of large follicles, LH receptor (LHR) mRNA levels were greater in the late than in the early follicular phase (p < 0.001, p < 0.05, for ROM and IF, respectively). In the early follicular phase, LHR levels in granulosa (p < 0.001) and theca cells (p < 0.05) of small follicles were greater in ROM than in IF ewes. FSH receptor mRNA levels in granulosa cells of small and large ROM follicles were greater than in the corresponding IF follicles (p < 0.05). Finally, a greater responsiveness (increase in cAMP secretion) to both FSH and hCG was observed by granulosa cells collected during the early follicular phase from ROM vs. IF ewes. Data provide evidence that the greater ovulation rate in the ROM as compared to the IF breed is associated with a greater gonadotropin responsiveness during the early follicular phase.

show abstract

Section: In Situ Hybridizationmentioning

confidence: 99%

Comparative Expression of Luteinizing Hormone and Follicle-Stimulating Hormone Receptors in Ovarian Follicles from High and Low Prolific Sheep Breeds1

Abdennebi¹,

Monget²,

Pisselet³

et al. 1999

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…PCR conditions and primer sequences for each studied gene are reported in Table 2 [6,24,[26][27][28][29][30][31][32]. RT-PCR products were electrophoresed in agarose gel; they were then Southern blotted and probed with a [␥-32 P]ATP-radiolabeled internal oligonucleotide.…”

Section: Rt-pcrmentioning

confidence: 99%

Reverse Transcription-Polymerase Chain Reaction Analysis of Genes Involved in Gonadal Differentiation in Pigs1

Parma

Pailhoux

Cotinot

1999

Biology of Reproduction

View full text Add to dashboard Cite

In mammals, testis development is initiated in the embryo as a response to the expression of the sex-determining gene, SRY. The time course of SRY expression during gonadal differentiation in the male has been described in detail only in mice and sheep. In this study, we used reverse transcription-polymerase chain reaction analysis to define the SRY transcription profile in pig genital ridges. SRY transcripts were first detectable from 23 days postcoitum (dpc), then declined sharply after 35 dpc. None were detected at 60 dpc. In addition, we analyzed temporal expression of other genes known to be involved in mammalian sex determination: WT-1, SF-1, SOX9, and AMH. A key stage seems to be 28 dpc, in which SOX9 expression switches between the male and female, and AMH expression begins to attest to Sertoli cell differentiation and to correspond to seminiferous cord formation in the male. Expression of gonadotropin receptors and aromatase was also investigated in porcine gonads, and we showed that their transcripts were detected very early on, especially in the male: 25 dpc for the LH receptor (rLH) and aromatase, and 28 dpc for the FSH receptor (rFSH). In the female, aromatase transcripts were not detected until 70 dpc, and rFSH expression occurred later: at 45 dpc at the onset of meiosis. Moreover, no difference was observed between the sexes for the onset of rLH transcription at 25 dpc. Such a thorough study has never been performed on pigs; developmental analysis will be useful for investigating sex-reversed gonads and determining ontogeny in intersexuality, a common pathology in pigs.

show abstract

“…Recently, SNPs in the human FSHR gene have been identified and severely affects the process of gametogenesis and infertility (Gromoll et al, 1996;Ahda et al, 2005;Wunsch et al, 2005;De Leener et al, 2006). Although the FSHR gene was first investigated as a potential candidate gene influencing NBA in pig in 1995 (Remy et al, 1995), only a few researchers have done further investigation on SNPs of this gene. Zhang et al (2002) performed an association study of SNP located at exon7 in the FSHR gene with size of reproductive organ and revealed sows that could provide more piglets with bigger reproductive organs usually carried BB genotype on the site Table 2 Least-squares mean and standard error for TN and NBA affected by genotype of FSHR gene.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we have focused on identifying associations between different reproductive phenotypes of pigs with the genotypes at single-nucleotide polymorphisms (SNPs) in two genes, namely the folliclestimulating hormone receptor (FSHR) and the zona pellucida 3 glycoprotein (ZP3) genes. FSHR in pig is a candidate gene which influences sow reproductive traits (Remy et al, 1995;Zhang et al, 2002). ZP3 in pig is an extracellular matrix protein which surrounds eggs and early embryos and is critical for normal fertilisation and pre-implantation development (David, 1997).…”

Section: Introductionmentioning

confidence: 99%

Association of four new single-nucleotide polymorphisms in follicle-stimulating hormone receptor and zona pellucida glycoprotein with reproductive traits in pigs

Yuan

Jafer

Affara

et al. 2007

Animal

View full text Add to dashboard Cite

Two new single-nucleotide polymorphisms (SNPs) (C1166T and G1190A) were discovered in the follicle-stimulating hormone receptor (FSHR) gene and two (G261A and T302C) in the zona pellucida glycoprotein (ZP3) gene. These SNPs were genotyped in three Chinese domestic purebred sow lines (42 Small Meishan, 46 Qingping and 41 Jinhua sows) and three European purebred sow lines (225 Duroc, 195 Large White and 65 Landrace sows) by using SNP chips. Phenotypic data including the functional teat number (i.e. milk-producing teats, TN) and number of piglets born alive per litter (NBA). These traits were tested for association with the genotypes of four SNPs. The association analysis revealed genotype of G261A in the ZP3 gene was significantly (P , 0.01) associated with overall NBA and NBA at later parities (NBA 21 ) but not with NBA at first parity (NBA 1 ). There was a significant (P , 0.05) difference between sows with genotype GG (14.83 6 0.18) and AA (14.26 6 0.09) in TN at position 261 in the ZP3 gene. No significant associations were observed for the SNPs in the FSHR gene with NBA or TN in our populations. The results showed that the new SNPs in the ZP3 gene may be an effective potential marker to be used in conjunction with traditional selection methods.

show abstract

The porcine follitropin receptor: cDNA cloning, functional expression and chromosomal localization of the gene

Cited by 27 publications

References 17 publications

Comparative Expression of Luteinizing Hormone and Follicle-Stimulating Hormone Receptors in Ovarian Follicles from High and Low Prolific Sheep Breeds1

Comparative Expression of Luteinizing Hormone and Follicle-Stimulating Hormone Receptors in Ovarian Follicles from High and Low Prolific Sheep Breeds1

Reverse Transcription-Polymerase Chain Reaction Analysis of Genes Involved in Gonadal Differentiation in Pigs1

Association of four new single-nucleotide polymorphisms in follicle-stimulating hormone receptor and zona pellucida glycoprotein with reproductive traits in pigs

Contact Info

Product

Resources

About