The plasma exosomal miR-1180-3p serves as a novel potential diagnostic marker for cutaneous melanoma

Guo, Yeye; Zhang, Xu; Wang, Linconghua; Li, Min; Shen, Minxue; Zhou, Zhe; Zhu, Susi; Li, Keke; Fang, Zhiqin; Yan, Bei; Zhao, Shuang; Su, Juan; Chen, Xiang; Peng, Cong

doi:10.1186/s12935-021-02164-8

Cited by 27 publications

(22 citation statements)

References 45 publications

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“…So far, the number of studies on EV embedded miRNAs of melanomas as biomarkers is limited. miR-1180-3p was reported as potential exosomal diagnostic marker, but in contrast to the miRNAs reported here, this miRNA was shown to be reduced in melanoma patients ( 72 ). The miR-222 was proposed as a diagnostic marker in melanoma, but it has not been analysed in patient-derived EVs, but only in cell lines derived from patients ( 82 ).…”

Section: Discussioncontrasting

confidence: 98%

Comprehensive Analyses of miRNAs Revealed miR-92b-3p, miR-182-5p and miR-183-5p as Potential Novel Biomarkers in Melanoma-Derived Extracellular Vesicles

et al. 2022

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Extracellular vesicles (EVs) are important mediators in the intercellular communication, influencing the function and phenotype of different cell types within the tumor micro-milieu and thus promote tumor progression. Since EVs safely transport packages of proteins, lipids and also nucleic acids such as miRNAs, EVs and their cargo can serve as diagnostic and prognostic markers. Therefore, the aim of this study was to investigate EV embedded miRNAs specific for melanoma, which could serve as potential biomarkers. In contrast to previous studies, we not only analysed miRNAs from EVs, but also included the miRNA profiles from the EV-secreting cells to identify candidates as suitable biomarkers. While the characterization of EVs derived from normal melanocytes and melanoma cells showed largely comparable properties with regard to size distribution and expression of protein markers, the NGS analyses yielded marked differences for several miRNAs. While miRNA load of EVs derived from normal human epidermal melanocytes (NHEMs) and melanoma cells were very similar, they were highly different from their secreting cells. By comprehensive analyses, six miRNAs were identified to be enriched in both melanoma cells and melanoma cell-derived EVs. Of those, the accumulation of miR-92b-3p, miR-182-5p and miR-183-5p in EVs could be validated in vitro. By functional network generation and pathway enrichment analysis we revealed an association with different tumor entities and signaling pathways contributing melanoma progression. Furthermore, we found that miR-92b-3p, miR-182-5p and miR-183-5p were also enriched in EVs derived from serum of melanoma patients. Our results support the hypothesis that miRNAs derived from EVs can serve as prognostic or diagnostic liquid biopsy markers in melanoma. We identified EV-derived miRNAs and showed that those miRNAs, which were enriched in melanoma cells and EVs, are also found elevated in serum-derived EVs of patients with metastatic melanoma, but not in healthy subjects.

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Section: Discussioncontrasting

confidence: 98%

Comprehensive Analyses of miRNAs Revealed miR-92b-3p, miR-182-5p and miR-183-5p as Potential Novel Biomarkers in Melanoma-Derived Extracellular Vesicles

et al. 2022

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“…Using ROC curve analysis of expression level in blood samples, miR-1180-3p was verified to owning the discriminating ability between CRC and healthy participants. In previous studies, miR-1180-3p has showed its diagnostic value in melanoma, gastric cancer, and early hepatocellular carcinoma [ 21 , 30 , 31 ]. This finding would shed light on the development of miR-1180-3p as a new noninvasive diagnostic marker for CRC.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, miR-145-5p could inhibit PLD5 resulting in downregulation of cell proliferation and metastasis in prostate cancer [ 36 ]. miR-1180-3p has been reported involving in the regulation of C11of54 in hepatocellular carcinoma and ST3GAL4 in cutaneous melanoma [ 30 , 37 ]. The search for gene targets of miR-1180-3p rendered COL12A1 by three online databases.…”

Section: Discussionmentioning

confidence: 99%

Clinical significance of microRNA-1180-3p for colorectal cancer and effect of its alteration on cell function

Zhang

Tian

2021

Bioengineered

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An early diagnosis and effective prognostic factors would greatly reduce the mortality rate of colorectal cancer (CRC). This research is intended to complete the evaluation of the prognostic value and potential role of miR-1180-3p in CRC. The miR-1180-3p levels were reduced in CRC patients’ tissues, blood, and human CRC cell lines. The ability of miR-1180-3p was explored in discrimination of CRC patients and healths and the value in overall survival estimate. The effect of miR-1180-3p dysregulation on the CRC cellular function was investigated. miR-1180-3p is downregulated in CRC tissues, blood and cells than normal ones. This lower expression was correlated with vascular invasion, lymph node metastasis, and TNM stage. With the use of ROC curve, miR-1180-3p showed discriminating ability in CRC patients and healthy subjects. With the result of Kaplan–Meier analysis and multi-multivariate Cox analysis, miR-1180-3p was an independent predictor for CRC patients’ overall survival. Utilizing CCK-8, Transwell and matrigel assays, overexpression of miR-1180-3p reduced cancer cell proliferation and mobility, but induced apoptosis, by targeting COL12A1 . miR-1180-3p might function as a suppressor in CRC progression and allowed the discovery of a new biomarker for diagnosis, prognosis and therapy target for CRC.

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“…Of note, in another study, EMT regulators miR-191 and let-7a were significantly higher in serum-derived sEVs of stage I melanoma patients compared to control patients in a cohort of 41 patients (20 nonmelanoma vs. 21 stage I melanoma) [121]. In a more recent study, RNA-seq of plasma-derived sEVs from a patient cohort of 20 patients per group (melanoma vs. healthy control patients) and a validation cohort of 28 patients per group revealed miR-1180-3p as a novel potential diagnostic marker for cutaneous melanoma [122] with an area under the curve (AUC) value of 0.729. In functional experiments, miR-1180-3p reduced proliferation, migration, and invasion of melanoma cells and was negatively regulated by ST3GAL4 [122].…”

Section: Ev-derived Mirna Profiling Of Melanoma Liquid Biopsiesmentioning

confidence: 98%

“…In a more recent study, RNA-seq of plasma-derived sEVs from a patient cohort of 20 patients per group (melanoma vs. healthy control patients) and a validation cohort of 28 patients per group revealed miR-1180-3p as a novel potential diagnostic marker for cutaneous melanoma [122] with an area under the curve (AUC) value of 0.729. In functional experiments, miR-1180-3p reduced proliferation, migration, and invasion of melanoma cells and was negatively regulated by ST3GAL4 [122]. A further study revealed a potential homeostatic role of sEVs in regulating miR-494.…”

Section: Ev-derived Mirna Profiling Of Melanoma Liquid Biopsiesmentioning

confidence: 99%

The Role of Extracellular Vesicles in Melanoma Progression

Lattmann

Levesque

2022

Cancers

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Cutaneous melanoma arises from a malignant transformation of the melanocytes in the skin. It is the deadliest form of skin cancer owing to its potential to metastasize. While recent advances in immuno-oncology have been successful in melanoma treatment, not all the patients respond to the treatment equally, thus individual pre-screening and personalized combination therapies are essential to stratify and monitor patients. Extracellular vesicles (EVs) have emerged as promising biomarker candidates to tackle these challenges. EVs are ~50–1000-nm-sized, lipid bilayer-enclosed spheres, which are secreted by almost all cell types, including cancer cells. Their cargo, such as nucleic acids, proteins, lipids, amino acids, and metabolites, can be transferred to target cells. Thanks to these properties, EVs can both provide a multiplexed molecular fingerprint of the cell of origin and thus serve as potential biomarkers, or reveal pathways important for cancer progression that can be targeted pharmaceutically. In this review we give a general overview of EVs and focus on their impact on melanoma progression. In particular, we shed light on the role of EVs in shaping the tumor–stroma interactions that facilitate metastasis and summarize the latest findings on molecular profiling of EV-derived miRNAs and proteins that can serve as potential biomarkers for melanoma progression.

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The plasma exosomal miR-1180-3p serves as a novel potential diagnostic marker for cutaneous melanoma

Cited by 27 publications

References 45 publications

Comprehensive Analyses of miRNAs Revealed miR-92b-3p, miR-182-5p and miR-183-5p as Potential Novel Biomarkers in Melanoma-Derived Extracellular Vesicles

Comprehensive Analyses of miRNAs Revealed miR-92b-3p, miR-182-5p and miR-183-5p as Potential Novel Biomarkers in Melanoma-Derived Extracellular Vesicles

Clinical significance of microRNA-1180-3p for colorectal cancer and effect of its alteration on cell function

The Role of Extracellular Vesicles in Melanoma Progression

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