The placental vascular endothelial growth factor polymorphisms and preeclampsia/preeclampsia severity

Keshavarzi, Farshid; Mohammadpour‐Gharehbagh, Abbas; Sheikhi, Mahnaz; Teimoori, Batool; Yazdi, Atefeh; Yaghmaei, Minoo; Naroeei-Nejad, Mehrnaz; Salimi, Saeedeh

doi:10.1080/10641963.2017.1299751

Cited by 15 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Early diagnosis would enable closer monitoring and treatment of this condition, and may open the window for earlier intervention that might help reduce the associated risks to the fetus and the mother. In addition to investigations studying circulating free proteins and microRNAs for their biomarker potential, plasma extracellular vesicles, including exosomes, have been extensively studied in many medical fields for their diagnostic potential [38][39][40][41][42][43][44][45][46][47][48][49][50] .…”

Section: Discussionmentioning

confidence: 99%

Syncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia

Levine

Habertheuer

Ram

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Preeclampsia is the most common placental pathology in pregnant females, with increased morbidity and mortality incurred on the mother and the fetus. There is a need for improved biomarkers for diagnosis and monitoring of this condition. Placental syncytiotrophoblasts at the maternal-fetal interface release nanoparticles, including extracellular microvesicles, into the maternal blood during pregnancy. Syncytiotrophoblast extracellular microvesicles (STEVs) are being studied for their diagnostic potential and for their potential physiologic role in preeclampsia. We hypothesized that STEV profiles in maternal circulation would be altered under conditions of preeclampsia compared to normal pregnancy. Extracellular vesicles (EVs) released by BeWo cells in vitro showed high expression of syncytin-1, but no plac1 expression, demonstrating that trophoblast cell EVs express syncytin-1 on their surface. Placental alkaline phosphatase also showed high expression on BeWo EVs, but due to concern for cross reactivity to highly prevalent isoforms of intestinal and bone alkaline phosphatase, we utilized syncytin-1 as a marker for STEVs. In vivo, syncytin-1 protein expression was confirmed in maternal plasma EVs from Control and Preeclampsia subjects by Western blot, and overall, lower expression was noted in samples from patients with preeclampsia (n = 8). By nanoparticle analysis, EV profiles from Control and Preeclampsia groups showed similar total plasma EV quantities (p = 0.313) and size distribution (p = 0.415), but STEV quantitative signal, marked by syncytin-1 specific EVs, was significantly decreased in the Preeclampsia group (p = 2.8 × 10 −11). Receiver operating characteristic curve demonstrated that STEV signal threshold cutoff of <0.316 was 95.2% sensitive and 95.6% specific for diagnosis of preeclampsia in this cohort (area under curve = 0.975 ± 0.020). In conclusion, we report that the syncytin-1 expressing EV profiles in maternal plasma might serve as a placental tissue specific biomarker for preeclampsia.

show abstract

Section: Discussionmentioning

confidence: 99%

Syncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia

Levine

Habertheuer

Ram

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Moreover, placental −634 GC and CC genotypes might be involved in the development of preeclampsia and also in its severe form [ 46 ], with OR 1.85 (95%CI 1.25–2.75) and OR 1.90 (95%CI 1.28–2.83) in the maternal and fetal dominant model [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

DNA Polymorphisms in Pregnant Women with Sticky Platelet Syndrome

Stančiaková

Žolková

Vadelová

et al. 2022

JCM

View full text Add to dashboard Cite

Sticky platelet syndrome (SPS) is a thrombophilia caused by the increased aggregability of platelets in response to the addition of low concentrations of epinephrine (EPI) and/or adenosine diphosphate (ADP). Some of the single nucleotide polymorphisms (SNP), alleles and haplotypes of platelet glycoprotein receptors were proved to have a role in the etiology of thrombotic episodes When comparing SPS and the control group, in VEGFA rs3025039, the p value for both CC vs. TT and CT vs. TT analyses was <0.001. Interestingly, no minor TT genotype was present in the SPS group, suggesting the thrombotic pathogenesis of recurrent spontaneous abortions (RSA) in these patients. Moreover, we found a significant difference in the presence of AT containing a risky A allele and TT genotype of ALPP rs13026692 (p = 0.034) in SPS patients when compared with the controls. Additionally, we detected a decreased frequency of the GG (CC) genotype of FOXP3 rs3761548 in patients with SPS and RSA when compared with the control group (p value for the CC (GG) vs. AA (TT) 0.021). This might indicate an evolutionary protective mechanism of the A (T) allele in the SPS group against thrombotic complications in pregnancy. These results can be used for antithrombotic management in such pregnant patients.

show abstract

“…PE is a complicated genetic disorder influenced by maternal and/or fetal genetic variants (32). Several genes are candidated for PE susceptibility (33,34), but no particular gene is introduced as the unique risk factor for PE (35). Although the association between different polymorphisms in maternal genes and PE has been reported, the correlation between placental polymorphisms and PE predisposition is reported in a few studies (36,37).…”

Section: Discussionmentioning

confidence: 99%

Impact of HOTAIR variants on preeclampsia susceptibility based on blood and placenta and in silico analysis

Mohammadpour‐Gharehbagh

Jahantigh

Saravani

et al. 2019

IUBMB Life

Self Cite

View full text Add to dashboard Cite

HOX transcript antisense RNA (HOTAIR) as a lncRNA involves in epigenetic regulation of various genes. Several studies have been suggested the effects of HOTAIR polymorphisms on different diseases. The aim of the present study was to evaluate the effect of maternal and placental HOTAIR polymorphisms on risk of preeclampsia (PE). The maternal blood of 203 preeclamptic and 202 nonpreeclamptic pregnant women as well as the placentas of 87 of preeclamptic and 95 nonpreeclamptic pregnant women were genotyped for HOTAIR polymorphisms. There was no association between maternal and placental HOTAIR polymorphisms (rs12826786, rs920778, and rs1899663) and PE risk. However, the maternal rs4759314AG and dominant model genotypes were associated with increased risk of PE. The maternal and placental HOTAIR rs10783618 polymorphism was associated with PE risk in recessive and allelic models. Haplotype analysis showed that, the maternal CTGAT and CCTAT and placental CTGAT haplotypes were significantly higher and maternal CTGAC, TCTAT, and TTGAT and placental CTGAC haplotypes were significantly lower in PE women. In silico analysis revealed that HOTAIR rs1899663 had a main effect on the secondary structure of mRNA, however, HOTAIR rs4759314 variant had potential alteration of splicing. In conclusion, the maternal and placental HOTAIR rs10783618 polymorphism might increase PE susceptibility. © 2019 IUBMB Life, 71(9):1367–1381, 2019

show abstract

The placental vascular endothelial growth factor polymorphisms and preeclampsia/preeclampsia severity

Cited by 15 publications

References 32 publications

Syncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia

Syncytiotrophoblast extracellular microvesicle profiles in maternal circulation for noninvasive diagnosis of preeclampsia

DNA Polymorphisms in Pregnant Women with Sticky Platelet Syndrome

Impact of HOTAIR variants on preeclampsia susceptibility based on blood and placenta and in silico analysis

Contact Info

Product

Resources

About