The pigments of some further marine phytoplankton species

Abstract

Thin-layer chromatography on plates coated with silica gel and with sucroseglucose (1:1) has been used in the study of the pigments of twenty species of marine phytoplankton from four phyla. A number of apparently new xanthophylls have been found, including a fucoxanthin-like pigment which is the major xanthophyll in an unidentified coccoid alga (Plymouth no. 407).

SHORT COMMUNICATION. The Effects of Copper and Zinc on the Fatty Acids and Carotenoids in the Marine Diatom Asterionella japonica

“…Four of the samples had the expected composition for diatoms with fucoxanthin äs the major pigment and ß,ß-carotene, diatoxanthin and diadinoxanthin also present. These four analyses are consistent with the previous study of the carotenoids in A. japonica (Riley and Segar 1969). The zinc-treated culture (expt.…”

“…Cells were harvested during late log-phase growth by filtration, extracted with chloroform/methanol (2:1, plus 0.05% pyridine), and analysed for fatty acids by capillary GC, and carotenoids by normal-phase HPLC, s described previously (Gillan et al 1981, Gillan andJohns, 1983). Fatty -acids and carotenoids were identified by comparison of their Chromatographie behaviour with that of Standards and by comparison with previously reported analyses of A. japonica (Chuecas andSegar 1969). Three experiments were performed on different dates with different batches of seawater.…”

Pigments, polysaccharides, and photosynthetic products of Phaeosaccion collinsii

“…This unidentified carotene was more strongly sorbed than F- Fucoxa~lthin from all algae examined was chromatographically identical. The absorption maxima recorded at nm 423, 447, 475 petrol; (429), 450, (474) EtOH, were similar in all cases and were in close agreement with published values (Riley and Segar 1969;Davies 1965).…”

“…Ochron~onus stipitata and Ruttnera sp. (both Chrysophyceae, Ochromonadales) are reported to contain violaxanthin ill lieu of diadinoxanthin (Hager and Stransky 1970b;Riley and Segar 1969). Our finding of violaxanthin in P. collinsii zoospores does not, therefore, give the species a unique status anlong the chrysophycean algae.…”

“…Pigmented material was exposed only to dim light and solutions of pigments were stored where necessary in darkness, under nitrogen in a refrigerator. Pigment concentrates were Riley 1969). The carotenoids were eluted by 10% methanol in diethyl ether while chlorophylls a and c were recovered with 90Y0 acetone and methanol respectively.…”