The physiology of infection with nematodes: The role of intracellular pH in the development of the early parasitic stage

External stimuli and intracellular signalling in the modification of the nematode surface during transition to the mammalian host environment

“…The fact that inhibition with amiloride was incomplete indicates that other antiporter systems could be involved, such as those for Na + /HCO 3 7H + /Cr, K + /H + and CT/HCCV which have been shown to control pH, (Thomas, 1984;Moolenaar, 1986). HCO 3~ itself is thought to be an important stimulus to nematodes in infection (Petronijevic & Rogers, 1987), and Fig. 1 shows that HCO 3 " alone is almost as effective as RPMI 1640 medium in promoting AF18 insertion into the surface of post-infective larvae.…”

External stimuli and intracellular signalling in the modification of the nematode surface during transition to the mammalian host environment

“…The fact that inhibition with amiloride was incomplete indicates that other antiporter systems could be involved, such as those for Na + /HCO 3 7H + /Cr, K + /H + and CT/HCCV which have been shown to control pH, (Thomas, 1984;Moolenaar, 1986). HCO 3~ itself is thought to be an important stimulus to nematodes in infection (Petronijevic & Rogers, 1987), and Fig. 1 shows that HCO 3 " alone is almost as effective as RPMI 1640 medium in promoting AF18 insertion into the surface of post-infective larvae.…”

Acidic intracellular pH shift during Caenorhabditis elegans larval development.

“…Regulation of pHi may be a key event involving growth factor-associated protein phosphorylation in mammals (6). Recent studies with third-stage "infective juveniles" of the parasitic nematode Haemonchus contortus suggest that the host agents H2CO3 and HCO may initiate Ca2+-dependent development by inducing changes in pH, (28). Since third-stage infective larvae of many parasitic nematodes are developmentally analogous to dauer larvae (29), an understanding of the cellular physiology of the C. elegans dauer larva might be applicable to parasite control.…”

Developmental arrest: from sea urchins to seeds

“…In unicellular systems such as sea urchin and Xenopus eggs, one of the early events of fertilization or artificial activation is an increase in intracellular pH (Grainger et al, 1979;Whitaker and Steinhardt, 1982;Busa and Nuccitelli, 1984;Charbonneau and Grandin, 1989;Epel, 1989;Freeman and Ridgway, 1993;Miller and Epel, 1999). By contrast, intracellular pH decreases upon activation of dormant multicellular systems, such as diapause cysts of the brine shrimp, Artemia franciscana (Drinkwater and Crowe, 1987), larvae and juveniles of the nematodes, Caenorhabditis elegans and Haemonchus contortus (Petronijevic and Rogers, 1987b;Wadsworth and Riddle, 1988), and upon proliferation of Syrian hamster embryo cells (Isfort et al, 1993(Isfort et al, , 1995. In plants, intracellular pH is also higher in dormant than in non-dormant tissues, e.g.…”

External stimuli and intracellular signalling in the modification of the nematode surface during transition to the mammalian host environment

“…The fact that inhibition with amiloride was incomplete indicates that other antiporter systems could be involved, such as those for Na + /HCO 3 7H + /Cr, K + /H + and CT/HCCV which have been shown to control pH, (Thomas, 1984;Moolenaar, 1986). HCO 3~ itself is thought to be an important stimulus to nematodes in infection (Petronijevic & Rogers, 1987), and Fig. 1 shows that HCO 3 " alone is almost as effective as RPMI 1640 medium in promoting AF18 insertion into the surface of post-infective larvae.…”

Acidic intracellular pH shift during Caenorhabditis elegans larval development.

“…Regulation of pHi may be a key event involving growth factor-associated protein phosphorylation in mammals (6). Recent studies with third-stage "infective juveniles" of the parasitic nematode Haemonchus contortus suggest that the host agents H2CO3 and HCO may initiate Ca2+-dependent development by inducing changes in pH, (28). Since third-stage infective larvae of many parasitic nematodes are developmentally analogous to dauer larvae (29), an understanding of the cellular physiology of the C. elegans dauer larva might be applicable to parasite control.…”

Developmental arrest: from sea urchins to seeds

“…In unicellular systems such as sea urchin and Xenopus eggs, one of the early events of fertilization or artificial activation is an increase in intracellular pH (Grainger et al, 1979;Whitaker and Steinhardt, 1982;Busa and Nuccitelli, 1984;Charbonneau and Grandin, 1989;Epel, 1989;Freeman and Ridgway, 1993;Miller and Epel, 1999). By contrast, intracellular pH decreases upon activation of dormant multicellular systems, such as diapause cysts of the brine shrimp, Artemia franciscana (Drinkwater and Crowe, 1987), larvae and juveniles of the nematodes, Caenorhabditis elegans and Haemonchus contortus (Petronijevic and Rogers, 1987b;Wadsworth and Riddle, 1988), and upon proliferation of Syrian hamster embryo cells (Isfort et al, 1993(Isfort et al, , 1995. In plants, intracellular pH is also higher in dormant than in non-dormant tissues, e.g.…”

External stimuli and intracellular signalling in the modification of the nematode surface during transition to the mammalian host environment

“…The fact that inhibition with amiloride was incomplete indicates that other antiporter systems could be involved, such as those for Na + /HCO 3 7H + /Cr, K + /H + and CT/HCCV which have been shown to control pH, (Thomas, 1984;Moolenaar, 1986). HCO 3~ itself is thought to be an important stimulus to nematodes in infection (Petronijevic & Rogers, 1987), and Fig. 1 shows that HCO 3 " alone is almost as effective as RPMI 1640 medium in promoting AF18 insertion into the surface of post-infective larvae.…”

Acidic intracellular pH shift during Caenorhabditis elegans larval development.

“…Regulation of pHi may be a key event involving growth factor-associated protein phosphorylation in mammals (6). Recent studies with third-stage "infective juveniles" of the parasitic nematode Haemonchus contortus suggest that the host agents H2CO3 and HCO may initiate Ca2+-dependent development by inducing changes in pH, (28). Since third-stage infective larvae of many parasitic nematodes are developmentally analogous to dauer larvae (29), an understanding of the cellular physiology of the C. elegans dauer larva might be applicable to parasite control.…”

Developmental arrest: from sea urchins to seeds

“…In unicellular systems such as sea urchin and Xenopus eggs, one of the early events of fertilization or artificial activation is an increase in intracellular pH (Grainger et al, 1979;Whitaker and Steinhardt, 1982;Busa and Nuccitelli, 1984;Charbonneau and Grandin, 1989;Epel, 1989;Freeman and Ridgway, 1993;Miller and Epel, 1999). By contrast, intracellular pH decreases upon activation of dormant multicellular systems, such as diapause cysts of the brine shrimp, Artemia franciscana (Drinkwater and Crowe, 1987), larvae and juveniles of the nematodes, Caenorhabditis elegans and Haemonchus contortus (Petronijevic and Rogers, 1987b;Wadsworth and Riddle, 1988), and upon proliferation of Syrian hamster embryo cells (Isfort et al, 1993(Isfort et al, , 1995. In plants, intracellular pH is also higher in dormant than in non-dormant tissues, e.g.…”