The physical exercise-induced oxidative/inflammatory response in peripheral blood mononuclear cells: Signaling cellular energetic stress situations

Royes, Luis Fernando Freire; Rosa, Pamela Carvalho Da; Bertomeu, Judit Borras; Osiecki, Raúl

doi:10.1016/j.lfs.2023.121440

Cited by 4 publications

(1 citation statement)

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“…High-altitude mountaineering involves exposure to a hypobaric hypoxic environment, leading to a series of physiological, metabolic and immunological changes. Circulating immune cells, including monocytes, DCs, T cells, NK cells and B cells, function as "sentinel tissue" to monitor the body's response [71]. Previous studies have proposed peripheral blood lymphocytes as biosensors for whole-body responses to high altitudes [16].…”

Section: Discussionmentioning

confidence: 99%

Multi-omics Reveals Immune Response and Metabolic Profiles during High-Altitude Mountaineering

Yin,

Lv,

Yang

et al. 2024

Preprint

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SUMMARYThe physiological perturbations induced by high-altitude exposure in mountain climbers, manifesting as metabolic and immunologic deviations, have been previously reported but are not fully understood. In this study, we obtained longitudinal multi-omic profiles of blood samples for healthy mountain climbers during two mountaineering stages (acclimatization and extreme altitude mountaineering). Our integrative assay included metabolomics and lipidomics profiling of plasma coupled with single-cell transcriptomic analysis of 375,722 immune cells. Longitudinal analysis revealed dynamic immune response profiles, during the acclimatization period, characterized by the downregulation of inflammatory responses in monocytes and classical dendritic cells (cDCs) and an increase in the proportion of cytotoxic CD8+T cells with enhanced immune effector processes. In contrast, during extreme altitude mountaineering, the activation of inflammatory responses and impairment of T cell effector function were observed, concomitant with an increased cellular response to hypoxia and oxidative stress pathways. Furthermore, we found upregulated glycolysis and antioxidant gene expression during extreme altitude mountaineering, which was primarily orchestrated byHIF1AandNFE2L2, while decreased expression of these genes was observed in dysregulated plasmacytoid dendritic cells (pDCs). Finally, high-resolution plasma metabolic analysis revealed significant alterations in the metabolism of climbers, involving enhanced glutamine and fatty acid metabolism.

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Section: Discussionmentioning

confidence: 99%