The Phases or Types of H. Pertussis

Toomey, John A.; Ranta, Katherine; Robey, Lucile; McClelland, Joseph E.

doi:10.1093/infdis/57.1.49

Cited by 13 publications

(8 citation statements)

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“…No culture line was continued beyond the 10th subculture. A rough variant of the BRI strain was produced by transferring the smooth strain to nutrient 5% chocolate-agar (18,19).…”

Section: Methodsmentioning

confidence: 99%

“…Rough-type andserum preparation and agglutination. Rough strains of B. pertussis are known to be very poor antigens (10,19). To elicit an antibody response, intramuscular and subcutaneous depot injections were given in conjunction with iv injections of saline vaccine.…”

Section: Methodsmentioning

confidence: 99%

“…The effect upon cell yield and upon the biological properties of the cells of supplementing Cohen and Wheeler broth (2) with monosodium VOL. 19,1970 GLUTAMIC ACID L-glutamate is reported here. The possibility of replacing a proportion of the casein hydrolysate in Cohen and Wheeler broth (2) by monosodium L-glutamate was also examined.…”

mentioning

confidence: 88%

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Use of Glutamic Acid to Supplement Fluid Medium for Cultivation of Bordetella pertussis

Lane¹

1970

Applied Microbiology

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“…No culture line was continued beyond the 10th subculture. A rough variant of the BRI strain was produced by transferring the smooth strain to nutrient 5% chocolate-agar (18,19).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Use of Glutamic Acid to Supplement Fluid Medium for Cultivation of Bordetella pertussis

Lane¹

1970

Applied Microbiology

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“…Later workers, while confirming the variability of B. pertussis cultures, were not able to segregate all strains into four sharply distinct groups on the basis of serological differences (Shibley and Hoelscher 1934;Toomey et al 1935;Andersen 1953). Eldering et al (1957) proposed a scheme of 14 heat-labile agglutinogens plus a common heat-stable or 0-antigen to explain the serological relationships not only between various strains of B. pertussis but also between the three species B. pertussis, B. pnrapertussis, and B. bronchiseptica.…”

Section: Introductionmentioning

confidence: 95%

A reexamination of phase IV Bordetella pertussis

Aprile¹

1972

Can. J. Microbiol.

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Eight strains of Bordetella pertussis labeled as "phase IV" and received from different laboratories were examined for their cultural, biochemical, and immunological characteristics in comparison with four phase I strains. Five of the strains possessed cultural and biochemical characteristics agreeing with published information on phase IV B. pertussis. Two of the strains had properties more closely resembling those of Brucella melitensis and one resembled Lophomonas sp.None of the phase IV strains possessed the protective antigen (PA) or the histamine-sensitizing factor (HSF) and they showed little immunological relationship with one another when tested for agglutination by antisera to phase IV except for L-46, L-51, and L-84, which formed a homogeneous serological group. Certain phase I antisera showed some degree of cross-reactivity with phase IV strains but there was much variation between the individual phase IV strains. Conversely the phase IV antisera cross-reacted with phase I organisms.

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“…Leslie tion through 4 phases, Phase IV being the final rough, avirulent form. Shibley and Hoelscher,8 and Toomey,et al,9 distinguish sharply between S and R forms but are unable to differentiate 4 phases. The former were successful in maintaining S strains over a prolonged period on a medium rich in human blood.…”

mentioning

confidence: 98%