The Pathogen-Induced MATE Gene TaPIMA1 Is Required for Defense Responses to Rhizoctonia cerealis in Wheat

Su, Qiang; Rong, Wei; Zhang, Zengyan

doi:10.3390/ijms23063377

Cited by 6 publications

(4 citation statements)

References 73 publications

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“…To evaluate the effect of MeJA treatment on Dn1, we first examined the expression levels of TaPIEP1 (pathogen-induced ERF encoding gene) and TaPIMA1 (pathogen-induced MATE) genes, which were previously reported to be the marker genes in response to JA ( Dong et al., 2010 ; Su et al., 2022 ). The results showed that at 5°C and -10°C, the expression of TaPIEP1 and TaPIMA1 in the MeJA treatment group was significantly higher than that in the control group ( Figure 1A, B ).…”

Section: Resultsmentioning

confidence: 99%

Overexpression of TaMYC2 confers freeze tolerance by ICE-CBF-COR module in Arabidopsis thaliana

Wang

Xia

et al. 2022

Front. Plant Sci.

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Dongnongdongmai No.1 (Dn1) is one of the few winter wheat varieties that can successfully overwinter at temperatures as low as -25°C or even lower. To date, few researches were carried to identify the freeze tolerance genes in Dn1 and applied them to improve plant resistance to extreme low temperatures. The basic helix-loop-helix (bHLH) transcription factor MYC2 is a master regulator in JA signaling, which has been reported to involve in responses to mild cold stress (2°C and 7°C). We hypothesized that MYC2 might be part of the regulatory network responsible for the tolerance of Dn1 to extreme freezing temperatures. In this study, we showed that wheat MYC2 (TaMYC2) was induced under both extreme low temperature (-10°C and-25°C) and JA treatments. The ICE-CBF-COR transcriptional cascade, an evolutionary conserved cold resistance pathway downstream of MYC2, was also activated in extreme low temperatures. We further showed that overexpression of any of the MYC2 genes from Dn1 TaMYC2A, B, D in Arabidopsis led to enhanced freeze tolerance. The TaMYC2 overexpression lines had less electrolyte leakage and lower malondialdehyde (MDA) content, and an increase in proline content, an increases antioxidant defences, and the enhanced expression of ICE-CBF-COR module under the freezing temperature. We further verified that TaMYC2 might function through physical interaction with TaICE41 and TaJAZ7, and that TaJAZ7 physically interacts with TaICE41. These results elucidate the molecular mechanism by which TaMYC2 regulates cold tolerance and lay the foundation for future studies to improve cold tolerance in plants.

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Section: Resultsmentioning

confidence: 99%

Overexpression of TaMYC2 confers freeze tolerance by ICE-CBF-COR module in Arabidopsis thaliana

Wang

Xia

et al. 2022

Front. Plant Sci.

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“…However, unlike EDS5, in A. thaliana EDS5H is constitutively expressed in green tissues independent of pathogen infection and does not contribute to pathogen-induced SA accumulation (Parinthawong et al ., 2015). Nevertheless, MATE proteins transport a broad range of, such as organic acids, plant hormones, and secondary metabolites, which could influence QDR (Nawrath et al ., 2002; Takanashi, et al ., 2014; Su, et al ., 2022). Conversely, BBX18, as well as the additional XK1 and FBA1 genes in the potential deletion linked to resistance, lack documented defense associations.…”

Section: Discussionmentioning

confidence: 99%

Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus)

Jacott¹,

Schoonbeek²,

Steuernagel³

et al. 2023

Preprint

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Crops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. Here, we use association transcriptomics (AT) to identify candidate gene loci associated withBrassica napusQDR to four contrasting fungal pathogens:Alternaria brassicicola,Botrytis cinerea,Pyrenopezia brassicaeandVerticillium longisporum. We observed broad-spectrum QDR dependent on the lifestyle of the fungal pathogen—hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci—suggesting potential trade-offs associated with such molecular determinants. We observed some loci involved in both early immune signalling and downstream QDR. In addition, we identify a novel QDR locus associated with resistance toV. longisporum. This is the first time AT has been used simultaneously for several pathosystems to identify loci involved in broad-spectrum QDR. As interesting candidates for crop breeding, we specifically highlight candidate loci for broad-spectrum QDR—to either the hemibiotrophic or necrotrophic pathogens—with no antagonistic effects on susceptibility to other pathogens.

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“…To address this, we utilized the FDR to manage the expected proportion of false positives among significant associations. FDR values for both GEM and GWA were determined using the q value R package (Storey 2011 ). Bonferroni thresholds for GWA and GEM are 6.64 and 6.03-Log 10 P, respectively, ( P < 0.05) and 6.34 and 5.73 −Log 10 P ( P < 0.1).…”

Section: Methodsmentioning

confidence: 99%

Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus)

Jacott,

Schoonbeek,

Sidhu

et al. 2024

Theor Appl Genet

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Key message Using associative transcriptomics, our study identifies genes conferring resistance to four diverse fungal pathogens in crops, emphasizing key genetic determinants of multi-pathogen resistance. Abstract Crops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. Here, we use associative transcriptomics (AT) to identify candidate gene loci associated with Brassica napus constitutive QDR to four contrasting fungal pathogens: Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae, and Verticillium longisporum. We did not identify any shared loci associated with broad-spectrum QDR to fungal pathogens with contrasting lifestyles. Instead, we observed QDR dependent on the lifestyle of the pathogen—hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci, including some loci associated with early immunity. Furthermore, we identify a genomic deletion associated with resistance to V. longisporum and potentially broad-spectrum QDR. This is the first time AT has been used for several pathosystems simultaneously to identify host genetic loci involved in broad-spectrum QDR. We highlight constitutive expressed candidate loci for broad-spectrum QDR with no antagonistic effects on susceptibility to the other pathogens studies as candidates for crop breeding. In conclusion, this study represents an advancement in our understanding of broad-spectrum QDR in B. napus and is a significant resource for the scientific community.

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The Pathogen-Induced MATE Gene TaPIMA1 Is Required for Defense Responses to Rhizoctonia cerealis in Wheat

Cited by 6 publications

References 73 publications

Overexpression of TaMYC2 confers freeze tolerance by ICE-CBF-COR module in Arabidopsis thaliana

Overexpression of TaMYC2 confers freeze tolerance by ICE-CBF-COR module in Arabidopsis thaliana

Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus)

Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus)

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