2020
DOI: 10.3390/ijms21093342
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Radiation therapy is one of the main methods of treating patients with non-small cell lung cancer (NSCLC). However, the resistance of tumor cells to exposure remains the main factor that limits successful therapeutic outcome. To study the molecular/cellular mechanisms of increased resistance of NSCLC to ionizing radiation (IR) exposure, we compared A549 (p53 wild-type) and H1299 (p53-deficient) cells, the two NSCLC cell lines. Using fractionated X-ray irradiation of these cells at a total dose of 60 Gy, we obt… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

2
17
0
1

Year Published

2020
2020
2022
2022

Publication Types

Select...
7

Relationship

2
5

Authors

Journals

citations
Cited by 19 publications
(20 citation statements)
references
References 57 publications
2
17
0
1
Order By: Relevance
“…In overall, the post-irradiation kinetics of change in γH2AX and 53BP1 numbers upon exposure to quasi-continuous radiation was in accordance to those reported in the literature for low LET radiation [ 22 , 24 ]. At 4 h post-irradiation about 45% of the γH2AX and 40% of 53BP1 foci of those observed at 1 h still were found, while at 24 h these values were 7 and 6%, respectively.…”
Section: Discussionsupporting
confidence: 89%
See 2 more Smart Citations
“…In overall, the post-irradiation kinetics of change in γH2AX and 53BP1 numbers upon exposure to quasi-continuous radiation was in accordance to those reported in the literature for low LET radiation [ 22 , 24 ]. At 4 h post-irradiation about 45% of the γH2AX and 40% of 53BP1 foci of those observed at 1 h still were found, while at 24 h these values were 7 and 6%, respectively.…”
Section: Discussionsupporting
confidence: 89%
“…This time-point was selected since it has been reported that the maximum number of γH2AX and 53BP1 foci is formed at 0.5–1 h post-irradiation [ 23 , 24 ]. For quasi-continuous electron irradiation, the quantitative yields of γH2AX and 53BP1 per Gy/cell are comparable to those reported in the literature for the exposures of cancer cells with low linear energy transfer (LET) radiation (17.6 and 16.4 foci/Gy/cell, respectively) [ 22 , 24 ]. After exposure with ultrashort pulsed irradiation at 2 and 20 Hz, the quantitative yield of foci was higher (about 1.1 and 1.3 times), though statistically significant changes were observed only at 20 Hz.…”
Section: Discussionsupporting
confidence: 71%
See 1 more Smart Citation
“…However, another uncertainty is whether radioresistance increased in association with the promotion of autophagy. Our previous [38] and current ( Figure 8) data indicated that both apoptosis and autophagy dose-dependently increased in the bulk of parental and MFR-surviving p53wt cells in response to acute IR exposure. On the contrary, the bulk of p53null cells possessed the decline of apoptosis and autophagy under the same conditions.…”
Section: Discussionmentioning
confidence: 73%
“…In our previous study [ 38 ], exponentially growing A549 (p53wt) and H1299 (p53null) cell lines were multiply irradiated by increasing doses of X-ray (ten fractions of 2 Gy, four fractions of 5 Gy, and two fractions of 10 Gy). The original parental cells were cultured without irradiation under the same conditions.…”
Section: Introductionmentioning
confidence: 99%