2013
DOI: 10.1111/febs.12528
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Abstract: The formate dehydrogenase from Rhodobacter capsulatus (RcFDH) is an oxygen-tolerant protein with an (abc) 2 . The preference for formate oxidation shows an energy barrier for CO 2 reduction of the enzyme. Furthermore, the FMN-containing and [Fe 4 S 4 ]-containing b-subunit together with the [Fe 2 S 2 ]-containing c-subunit forms a diaphorase unit with activities for both NAD + reduction and NADH oxidation. In addition to the structural genes fdsG, fdsB, and fdsA, the fds operon in R. capsulatus contains the f… Show more

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Cited by 128 publications
(180 citation statements)
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“…capsulatus FDH was expressed, purified and the cofactor content was determined as described previously (4).…”
Section: Site-directed Mutagenesis Expression and Purification And Cmentioning
confidence: 99%
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“…capsulatus FDH was expressed, purified and the cofactor content was determined as described previously (4).…”
Section: Site-directed Mutagenesis Expression and Purification And Cmentioning
confidence: 99%
“…In general, FDH enzymes were shown to be stabilized by the addition of nitrate during purification (4,17). We therefore investigated the effect of nitrate on the catalytic activity of FDH WT .…”
Section: The Effect Of Nitrate On Fdh Wt Stability and Activitymentioning
confidence: 99%
See 1 more Smart Citation
“…Besides Mo-nitrogenase containing FeMoco, the iron-molybdenum cofactor, R. capsulatus is capable of synthesizing the molybdoenzymes xanthine dehydrogenase (XDH), formate dehydrogenase (FDH), and aldehyde dehydrogenase (ALDH), containing the Moco molybdopterin cofactor (30)(31)(32). The XDH, FDH, ALDH, and Moco biosynthesis enzymes were expressed at very low levels or were not even identified in our data set (see Tables S1 and S2 in the supplemental material).…”
Section: Discussionmentioning
confidence: 99%
“…In R. capsulatus , one FDH is present in the cytoplasm [112]. The physiological role of the enzyme for R. capsulatus has not been resolved to date.…”
Section: Fes Cluster Containing Molybdoenzymes In Bacteriamentioning
confidence: 99%