The ouabain-insensitive sodium pump

Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way

“…Due to its abundance in the extracellular fluid, it is imperative to understand the mechanisms by which Na + is reabsorbed or excreted in urine because alterations in the interstitial and intravascular Na + concentrations lead to changes in volume and might cause disturbances in blood pressure [50]. The regulation of Na + levels in body liquid compartments are principally driven by primary active transporters with the classical Na + + K + -ATPase [51] being involved in mass transport, while the "second" Na + pump or Na + -ATPase is involved in the fine-tuned regulation of Na + reabsorption [52,53]. There are many reports in the literature concerning the regulation of Na + + K + -ATPase by hormones and autacoids [50,54] as well as the role of regulatory phosphorylation [50,55].…”

Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way

“…Due to its abundance in the extracellular fluid, it is imperative to understand the mechanisms by which Na + is reabsorbed or excreted in urine because alterations in the interstitial and intravascular Na + concentrations lead to changes in volume and might cause disturbances in blood pressure [50]. The regulation of Na + levels in body liquid compartments are principally driven by primary active transporters with the classical Na + + K + -ATPase [51] being involved in mass transport, while the "second" Na + pump or Na + -ATPase is involved in the fine-tuned regulation of Na + reabsorption [52,53]. There are many reports in the literature concerning the regulation of Na + + K + -ATPase by hormones and autacoids [50,54] as well as the role of regulatory phosphorylation [50,55].…”

Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: biochemical characteristics and differential modulation after feeding

“…In several vertebrate and invertebrate tissues, the insensitivity to ouabain (a well-established specific inhibitor of Na + -K + ATPase; Kaplan 2002;Nesher et al 2007;Miles et al 2013;Leone et al 2017) and the inhibition by furosemide (unlike Na + -K + ATPase) allowed to identify the occurrence of a K + -independent Na + ATPase activity ( Proverbio et al 1986Proverbio et al , 1991Caruso-Neves et al1999;Beltowski et al2004;Rocafull et al 2012;Vieyra et al 2016). By using this approach, in this work we showed, for the first time in a euryhaline crab, the occurrence of K + independent, ouabain-insensitive Na + ATPase activity in hepatopancreas of N. granulata.…”

Low salinity‐induced alterations in epithelial ultrastructure, Na+/K+‐ATPase immunolocalization and enzyme kinetic characteristics in the gills of the thinstripe hermit crab,Clibanarius vittatus(Anomura, Diogenidae)

“…Ouabain‐insensitive Na + ‐stimulated ATPase activity may play a role in cell volume regulation and in transepithelial Na + transport in various invertebrates ranging from freshwater shrimp ( Macrobrachium amazonicum , Proverbio, Zanders, Marin, Rodrigues, & Proverbio, ; M. olfersii , Lima, McNamara, & Terra, ) to bivalves ( Tapes philippinarum, Mytilus galloprovincialis , Pagliarani et al., ; Proverbio, Marin, & Proverbio, ), and in certain teleosts ( Dicentraxus labrax , Ventrella, Pagliarani, Trigari, Trombetti, & Borgatti, ; Oncorhynchus mykiss , Ventrella et al., ). Such Mg 2+ ‐dependent ouabain‐insensitive ATP activity represents ≈ 30% of total ATPase activity in C. vittatus gills, and its stimulation by Na + , K + or NH 4 + corroborates a role in the cellular regulation of monovalent cations.…”

Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way

“…Due to its abundance in the extracellular fluid, it is imperative to understand the mechanisms by which Na + is reabsorbed or excreted in urine because alterations in the interstitial and intravascular Na + concentrations lead to changes in volume and might cause disturbances in blood pressure [50]. The regulation of Na + levels in body liquid compartments are principally driven by primary active transporters with the classical Na + + K + -ATPase [51] being involved in mass transport, while the "second" Na + pump or Na + -ATPase is involved in the fine-tuned regulation of Na + reabsorption [52,53]. There are many reports in the literature concerning the regulation of Na + + K + -ATPase by hormones and autacoids [50,54] as well as the role of regulatory phosphorylation [50,55].…”

Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: biochemical characteristics and differential modulation after feeding

“…In several vertebrate and invertebrate tissues, the insensitivity to ouabain (a well-established specific inhibitor of Na + -K + ATPase; Kaplan 2002;Nesher et al 2007;Miles et al 2013;Leone et al 2017) and the inhibition by furosemide (unlike Na + -K + ATPase) allowed to identify the occurrence of a K + -independent Na + ATPase activity ( Proverbio et al 1986Proverbio et al , 1991Caruso-Neves et al1999;Beltowski et al2004;Rocafull et al 2012;Vieyra et al 2016). By using this approach, in this work we showed, for the first time in a euryhaline crab, the occurrence of K + independent, ouabain-insensitive Na + ATPase activity in hepatopancreas of N. granulata.…”

Low salinity‐induced alterations in epithelial ultrastructure, Na+/K+‐ATPase immunolocalization and enzyme kinetic characteristics in the gills of the thinstripe hermit crab,Clibanarius vittatus(Anomura, Diogenidae)

“…Ouabain‐insensitive Na + ‐stimulated ATPase activity may play a role in cell volume regulation and in transepithelial Na + transport in various invertebrates ranging from freshwater shrimp ( Macrobrachium amazonicum , Proverbio, Zanders, Marin, Rodrigues, & Proverbio, ; M. olfersii , Lima, McNamara, & Terra, ) to bivalves ( Tapes philippinarum, Mytilus galloprovincialis , Pagliarani et al., ; Proverbio, Marin, & Proverbio, ), and in certain teleosts ( Dicentraxus labrax , Ventrella, Pagliarani, Trigari, Trombetti, & Borgatti, ; Oncorhynchus mykiss , Ventrella et al., ). Such Mg 2+ ‐dependent ouabain‐insensitive ATP activity represents ≈ 30% of total ATPase activity in C. vittatus gills, and its stimulation by Na + , K + or NH 4 + corroborates a role in the cellular regulation of monovalent cations.…”

Ceramide-1-Phosphate as a Potential Regulator of the Second Sodium Pump from Kidney Proximal Tubules by Triggering Distinct Protein Kinase Pathways in a Hierarchic Way

“…Due to its abundance in the extracellular fluid, it is imperative to understand the mechanisms by which Na + is reabsorbed or excreted in urine because alterations in the interstitial and intravascular Na + concentrations lead to changes in volume and might cause disturbances in blood pressure [50]. The regulation of Na + levels in body liquid compartments are principally driven by primary active transporters with the classical Na + + K + -ATPase [51] being involved in mass transport, while the "second" Na + pump or Na + -ATPase is involved in the fine-tuned regulation of Na + reabsorption [52,53]. There are many reports in the literature concerning the regulation of Na + + K + -ATPase by hormones and autacoids [50,54] as well as the role of regulatory phosphorylation [50,55].…”

Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: biochemical characteristics and differential modulation after feeding

“…In several vertebrate and invertebrate tissues, the insensitivity to ouabain (a well-established specific inhibitor of Na + -K + ATPase; Kaplan 2002;Nesher et al 2007;Miles et al 2013;Leone et al 2017) and the inhibition by furosemide (unlike Na + -K + ATPase) allowed to identify the occurrence of a K + -independent Na + ATPase activity ( Proverbio et al 1986Proverbio et al , 1991Caruso-Neves et al1999;Beltowski et al2004;Rocafull et al 2012;Vieyra et al 2016). By using this approach, in this work we showed, for the first time in a euryhaline crab, the occurrence of K + independent, ouabain-insensitive Na + ATPase activity in hepatopancreas of N. granulata.…”

Low salinity‐induced alterations in epithelial ultrastructure, Na+/K+‐ATPase immunolocalization and enzyme kinetic characteristics in the gills of the thinstripe hermit crab,Clibanarius vittatus(Anomura, Diogenidae)

“…Ouabain‐insensitive Na + ‐stimulated ATPase activity may play a role in cell volume regulation and in transepithelial Na + transport in various invertebrates ranging from freshwater shrimp ( Macrobrachium amazonicum , Proverbio, Zanders, Marin, Rodrigues, & Proverbio, ; M. olfersii , Lima, McNamara, & Terra, ) to bivalves ( Tapes philippinarum, Mytilus galloprovincialis , Pagliarani et al., ; Proverbio, Marin, & Proverbio, ), and in certain teleosts ( Dicentraxus labrax , Ventrella, Pagliarani, Trigari, Trombetti, & Borgatti, ; Oncorhynchus mykiss , Ventrella et al., ). Such Mg 2+ ‐dependent ouabain‐insensitive ATP activity represents ≈ 30% of total ATPase activity in C. vittatus gills, and its stimulation by Na + , K + or NH 4 + corroborates a role in the cellular regulation of monovalent cations.…”