The otc gene of Streptococcus suis plays an important role in biofilm formation, adhesion, and virulence in a murine model

Li, Yang; Li, Jinpeng; Fan, Qingying; Mao, Chenlong; Jin, Muchun; Liu, Yichen; Sun, Lijun; Grenier, Daniel; Wang, Yang

doi:10.1016/j.vetmic.2020.108925

Cited by 16 publications

(9 citation statements)

References 29 publications

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“…We previously identified nine unique proteins in the biofilm of S. suis through comparative proteomics analysis [ 34 ]. Further research found that the pdh significantly up-regulates the adhesion and invasion ability of S. suis [ 13 ], and the otc can improve the pathogenicity in the mouse abdominal infection model [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies have shown that S. suis serotype 2 can regulate biofilm formation and virulence factor expression through the LuxS/AI-2 density sensing system, leading to a marked resistance to fluoroquinolones and tetracycline antibiotics [ 10 , 12 , 14 , 15 , 35 ]. Currently, there is little treatment option for infections caused by S. suis serotype 2, and consequently the search for antibiotic substitutes with the ability to inhibit bacteria in a biofilm state is an active field of research.…”

Section: Discussionmentioning

confidence: 99%

“…A mouse protection assay was performed according to a protocol previously published by our group [ 15 ]. Mice were intraperitoneally injected with 1 × 10 6 CFU/ml of S. suis to induce infection.…”

Section: Methodsmentioning

confidence: 99%

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Paeoniflorin reduce luxS/AI-2 system-controlled biofilm formation and virulence in Streptococcus suis

Fan

Jin

et al. 2021

Virulence

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Streptococcus suis ( S. suis ), more specifically serotype 2, is a bacterial pathogen that threatens the lives of pigs and humans. Like many other pathogens, S. suis exhibits quorum sensing (QS) system-controlled virulence factors, such as biofilm formation that complicates treatment. Therefore, impairing the QS involving LuxS/AI-2 cycle in S. suis , may be a promising alternative strategy for overcoming S. suis infections. In this study, we investigated paeoniflorin (PF), a monoterpenoid glycoside compound extracted from peony, as an inhibitor of S. suis LuxS/AI-2 system. At a sub-minimal inhibitory concentration (MIC) (1/16 MIC; 25 μg/ml), PF significantly reduced biofilm formation by S. suis through inhibition of extracellular polysaccharide (EPS) production, without affecting bacterial growth. Moreover, evidence was brought that PF reduces AI-2 activity in S. suis biofilm. Molecular docking indicated that LuxS may be the target of PF. Monitoring LuxS enzymatic activity confirmed that PF had a partial inhibitory effect. Finally, we showed that the use of PF in a mouse model can relieve S. suis infections. This study highlighted the anti-biofilm potential of PF against S. suis , and brought evidence that it may as an inhibitor of the LuxS/AI-2 system to prevent S. suis biofilm-related infections. PF can thus be used as a new type of natural biofilm inhibitor for clinical application.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Paeoniflorin reduce luxS/AI-2 system-controlled biofilm formation and virulence in Streptococcus suis

Fan

Jin

et al. 2021

Virulence

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“…The crystal violet microplate method was used for determination (Li et al 2021;Liu et al 2020). The critical point ODc value for judging whether the biofilm can be formed is 2 times the absorbance (OD) value of the negative control well (Wang et al 2016;Yi et al 2020).…”

Section: Biofilm Formation Ability Testmentioning

confidence: 99%

Phylogenetic Group, Biofilm Formation and Drug Resistance of Escherichia coli Isolated from Goose and Fish in Henan, China

Jia¹,

Cui²,

Ye³

et al. 2023

IJAR

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Background: Escherichia coli (E. coli) is a pathogen that sickens both humans and animals. There are relatively few reports on the correlation between drug resistance and biofilm formation ability of E.coli isolated from goose and fish in water environment of China. This study investigated the genetic typing, antibiotic sensitivity and biofilm formation ability of E. coli strains isolated from goose and fish. Methods: The phylogenetic clustering, drug resistance and biofilm formation ability of E. coli were tested by triple PCR, crystal violet microplate and Microdilution method. Result: The results showed the prevalence of group B2 and D in fish-derived E.coli accounted for 59% and 12%, goose-derived E. coli accounted for 48% and 9%, respectively. Some strains could form biofilm and biofilm formation ability was associated with the drug resistance. The MIC values of strains growing in biofilm were 2-16 times higher than those of corresponding planktonic bacteria. The antibiotic resistance among biofilm-forming isolates was significantly higher than that strains unable to form biofilm (p less than 0.05).

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“…Identification of an agent by the MALDI-TOF MS method requires the development of databases for accurate identification at the level of species and serotypes. Currently developed serological diagnostic methods are aimed at detecting only 2 serotypes of the pathogen that have the greatest zoonotic potential (Yi et al, 2020;Jiang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Detection of Streptococcus suis using the optimized real-time polymerase chain reaction protocol

Savcheniuk

Tarasov²,

Zakharova³

et al. 2022

Regul. Mech. Biosyst.

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The article presents the results of studies on the detection of Streptococcus suis by real-time polymerase chain reaction. Isolation and species identification of the studied isolates of streptococci was carried out according to morphological, cultural, biochemical and biological properties by conventional methods. The study of cultural characteristics of growth was carried out using conventional bacteriological methods on the brain heart infusion broth (BHI) and BHI agar with the addition of 5% sheep blood (blood BHI agar). To confirm biochemical properties as a confirmatory method, API 20 STREP test kit (bioMerieux, France) was used. In addition, to differentiate S. suis from the non-pathogenic species of streptococci, the hemolysis test was used. As a result of the studies, it was found that the use of the real-time PCR (polymerase chain reaction) method makes it possible to detect S. suis in an amount of 1 x 104 genome copies in the sample. All described validation parameters for the qualitative detection of S. suis DNA by real-time PCR meet international requirements, which guarantees accurate and reliable results. In Ukraine only a diagnostic test kit for convential PCR has been developed for the detection of swine streptococcosis. This approach is more time consuming and complex in comparison with the real-time PCR approach. We recommend that diagnostic laboratories implement this method in their practice. This will increase the number of effective diagnostic tools available to veterinarians on pig farms when they order laboratory tests. The high analytical sensitivity limit of a test is an essential parameter when screening is the focus, and obtaining false negative results causes a risk of the development of infection process among pig populations within infected herds. Our study showed that microbiological diagnostic methods to determine morphological and cultural properties can identify S. suis at the genus level. Determination of biochemical properties using the API 20 STREP test kit can be used to identify S. suis 1 and 2 serotypes. The conventional method and real-time PCR have 100% specificity and can be used to identify S. suis of different serotypes. Real-time PCR is a 2 to 4 times more sensitive limit than conventional PCR depending on the serotype being studied, and can be used to more accurately identify streptococcal DNA. It was found that the use of the real-time PCR method makes it possible to detect S. suis in an amount of 1 x 104 copies of the genome in the sample. Additionally, it was found that all the studied validation parameters of the qualitative method for determining S. suis DNA by real-time PCR meet international requirements, which guarantees accurate and reliable results.

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The otc gene of Streptococcus suis plays an important role in biofilm formation, adhesion, and virulence in a murine model

Cited by 16 publications

References 29 publications

Paeoniflorin reduce luxS/AI-2 system-controlled biofilm formation and virulence in Streptococcus suis

Paeoniflorin reduce luxS/AI-2 system-controlled biofilm formation and virulence in Streptococcus suis

Phylogenetic Group, Biofilm Formation and Drug Resistance of Escherichia coli Isolated from Goose and Fish in Henan, China

Detection of Streptococcus suis using the optimized real-time polymerase chain reaction protocol

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