The OST‐complex as target for RNAi‐based pest control in <i>Nilaparvata lugens</i>

Schutter, Kristof De; Chen, Pengyu; Shen, Ying; Damme, Els J. M. Van; Smagghe, Guy

doi:10.1002/arch.21555

Cited by 7 publications

(10 citation statements)

References 19 publications

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“…trimming caused a peak of mortality at the transition from nymph to adult. These results confirm the crucial role of N-glycan attachment for insect growth and survival as was previously observed in the holometabolous insects T. castaneum and D. melanogaster (Spradling et al, 1999;De Schutter et al, 2019). However, some differences between the phenotypes in holometabolous and hemimetabolous insects were observed.…”

Section: Tablesupporting

confidence: 90%

“…Similarly, RNAi-mediated silencing of the genes encoding for the enzymes involved in these essential steps causes high mortality in N. lugens. Blockage of the N-glycan attachment, by cosilencing of both Stt3 isoforms, resulted in high nymphal mortality (De Schutter et al, 2019). While RNAi of the glucosidases and interference with the high-mannose N-glycan…”

Section: Discussionmentioning

confidence: 99%

“…The sequences of 17 NGRG were identified by Scheys et al (2019) (Table S1). A functional analysis for 2 of these 17 NGRGs, namely the OST Stt3a and Stt3b subunits, was previously performed (De Schutter et al, 2019); therefore, this study was performed with the 15 remaining NGRGs, involved in the processing the N-glycan structure. Gene-specific dsRNAs were synthesized using the MEGAscript TM RNAi Kit (catalog no.…”

Section: Rnai and Microinjectionmentioning

confidence: 99%

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RNAi of the N‐glycosylation‐related genes confirms their importance in insect development and α‐1,6‐fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens

et al. 2021

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Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N-glycosylation in the development of insects, a functional characterization of the N-glycosylation-related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi-mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N-glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the α-1,6-fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene-silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.

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Section: Tablesupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Rnai and Microinjectionmentioning

confidence: 99%

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RNAi of the N‐glycosylation‐related genes confirms their importance in insect development and α‐1,6‐fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens

et al. 2021

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“…Another possible explanation is that due to the increased pressure by silencing three genes a compensation effect takes place. A similar observation was made when silencing the catalytic subunit of the N-glycosylation oligosaccharyl transferase complex, after silencing of one isoform, the expression of the other isoform increased (De Schutter et al, 2019). Nevertheless, it is interesting that all double or triple RNAi experiments involving TcOGT resulted in a significantly higher larval mortality than the single RNAi, while the larval mortality was only minor in the single RNAi of TcOGT.…”

Section: Functional Analysis Of Ogrgs During Post-embryonic Developmesupporting

confidence: 60%

RNAi-Mediated Silencing of Pgants Shows Core 1 O-Glycans Are Required for Pupation in Tribolium castaneum

Schutter

Damme

et al. 2021

Front. Physiol.

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Protein glycosylation is one of the most common and most important post-translational modifications. Despite the growing knowledge on N-glycosylation, the research on O-glycosylation is lagging behind. This study investigates the importance of O-glycosylation in the post-embryonic development of insects using the red flour beetle, Tribolium castaneum, as a model. We identified 28 O-glycosylation-related genes (OGRGs) in the genome of the red flour beetle. 14 OGRGs were selected for functional analysis based on their involvement in the initial attachment of the carbohydrate in the different O-glycosylation pathways or the further elongation of the most abundant O-glycans and, in addition, showing severe RNAi-induced phenotypes in Drosophila melanogaster. The expression profile of these OGRGs was mapped throughout the developmental stages of the insect and in the different tissues of the pupa and adult. Subsequently, these genes were silenced using RNA interference (RNAi) to analyze their role in development. A broad spectrum of phenotypes was observed: from subtle effects and disrupted wing formation when silencing the genes involved in O-mannosylation, to blockage of pupation and high mortality after silencing of the genes involved in O-GalNAc and core 1 O-glycan (O-GalNAc-Gal) synthesis. RNAi experiments were also performed to assess the effects of blocking multiple pathways of O-glycosylation. However, the observed phenotypes induced by multiple RNAi were similar to those of the single gene RNAi experiments. The silencing of OGRGs often resulted in high mortality and wing phenotypes, indicating the importance of O-glycosylation for the survival of the insect and the formation of wings during the post-embryonic development of T. castaneum.

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“…In the majority of the surveyed publications, only one target gene was used per bioassay, but multiple studies used two (for examples see the literature 41,51,52 ) and up to four target genes in a single RNAi assay. 53 The top five target genes by number observed, Vha, Ace, chitinase (Cht), COPI coatomer (COP), and cellulase (GHF) represent 24.5% of the data.…”

Section: Target Genesmentioning

confidence: 99%

RNA meets toxicology: efficacy indicators from the experimental design of RNAi studies for insect pest management

List

Tarone

Zhu-Salzman

et al. 2022

Pest Management Science

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RNA interference (RNAi) selectively targets genes and silences their expression in vivo, causing developmental defects, mortality and altered behavior. Consequently, RNAi has emerged as a promising research area for insect pest management. However, it is not yet a viable alternative over conventional pesticides despite several theoretical advantages in safety and specificity. As a first step toward a more standardized approach, a machine learning algorithm was used to identify factors that predict trial efficacy. Current research on RNAi for pest management is highly variable and relatively unstandardized. The applied random forest model was able to reliably predict mortality ranges based on bioassay parameters with 72.6% accuracy. Response time and target gene were the most important variables in the model, followed by applied dose, double‐stranded RNA (dsRNA) construct size and target species, further supported by generalized linear mixed effect modeling. Our results identified informative trends, supporting the idea that basic principles of toxicology apply to RNAi bioassays and provide initial guidelines standardizing future research similar to studies of traditional insecticides. We advocate for training that integrates genetic, organismal, and toxicological approaches to accelerate the development of RNAi as an effective tool for pest management. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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The OST‐complex as target for RNAi‐based pest control in Nilaparvata lugens

Cited by 7 publications

References 19 publications

RNAi of the N‐glycosylation‐related genes confirms their importance in insect development and α‐1,6‐fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens

RNAi of the N‐glycosylation‐related genes confirms their importance in insect development and α‐1,6‐fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens

RNAi-Mediated Silencing of Pgants Shows Core 1 O-Glycans Are Required for Pupation in Tribolium castaneum

RNA meets toxicology: efficacy indicators from the experimental design of RNAi studies for insect pest management

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