The optical rotator

Tandrup, Trine; Gundersen, Hans Jørgen G.; Jensen, Eva B. Vedel

doi:10.1046/j.1365-2818.1997.2070765.x

Cited by 113 publications

(88 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Granuloma volumes were estimated using optical rotator stereology (17). Schistosoma egg density in mouse lung tissue was determined using potassium hydroxide digestion (18).…”

Section: Image Analysis and Egg Burden Quantificationmentioning

confidence: 99%

The Causal Role of IL-4 and IL-13 inSchistosoma mansoniPulmonary Hypertension

Kumar

Mickael

Chabon

et al. 2015

Am J Respir Crit Care Med

106

View full text Add to dashboard Cite

Rationale: The etiology of schistosomiasis-associated pulmonary arterial hypertension (PAH), a major cause of PAH worldwide, is poorly understood. Schistosoma mansoni exposure results in prototypical type-2 inflammation. Furthermore, transforming growth factor (TGF)-b signaling is required for experimental pulmonary hypertension (PH) caused by Schistosoma exposure.Objectives: We hypothesized type-2 inflammation driven by IL-4 and IL-13 is necessary for Schistosoma-induced TGF-b-dependent vascular remodeling.Methods: Wild-type, IL-4 2/2

show abstract

“…Granuloma volumes were estimated using optical rotator stereology (17). Schistosoma egg density in mouse lung tissue was determined using potassium hydroxide digestion (18).…”

Section: Image Analysis and Egg Burden Quantificationmentioning

confidence: 99%

The Causal Role of IL-4 and IL-13 inSchistosoma mansoniPulmonary Hypertension

Kumar

Mickael

Chabon

et al. 2015

Am J Respir Crit Care Med

106

View full text Add to dashboard Cite

show abstract

“…24 Briefly, paraffin-embedded tissue was stained with H&E, and 10 to 12 images of granulomas were acquired for each sample. The rotator method for object volume estimation was then applied using the egg spine as the central reference point.…”

Section: Granuloma Volume Assessmentmentioning

confidence: 99%

Schistosomiasis-Induced Experimental Pulmonary Hypertension

Graham

Mentink‐Kane

El-Haddad

et al. 2010

The American Journal of Pathology

View full text Add to dashboard Cite

The mechanisms underlying schistosomiasis-induced pulmonary hypertension (PH), one of the most common causes of PH worldwide, remain unclear. We sought to determine whether Schistosoma mansoni causes experimental PH associated with pulmonary vascular remodeling in an interleukin (IL)-13-dependent manner. IL-13R␣1 is the canonical IL-13 signaling receptor, whereas IL-13R␣2 is a competitive nonsignaling decoy receptor. Wild-type, IL-13R␣1 ؊/؊ , and IL-13R␣2 ؊/؊ C57BL/6J mice were percutaneously infected with S. ؊/؊ mice. Phosphorylated Smad2/3, a target of transforming growth factor-␤ signaling, was increased in both infected mice and humans with the disease. Our data indicate that experimental schistosomiasis causes PH and potentially relies on up-regulated IL-13 signaling.

show abstract

“…The absolute number of neurons ( N) and the mean neuronal volume (v N ) and distribution of individual volume estimates were obtained in each TG by the application of the optical fractionator (West, 1993) and the planar rotator (Tandrup et al, 1997), respectively, over the same neurons systematically sampled. All measurements were performed using an interactive computer system consisting of a highprecision motorized microscope stage, a 0.5 m resolution z-axis reader (microcator VZR 401; Heidenhain, Traunreut, Germany), a solid-state video camera, and a high-resolution video monitor.…”

Section: Quantitative Studies In Adult Rat Tg In Vivomentioning

confidence: 99%

Primary Sensory Neuron Addition in the Adult Rat Trigeminal Ganglion: Evidence for Neural Crest Glio-Neuronal Precursor Maturation

Lagares¹,

Li²,

Zhou³

et al. 2007

J. Neurosci.

View full text Add to dashboard Cite

It is debated whether primary sensory neurons of the dorsal root ganglia increase the number in adult animals and, if so, whether the increase is attributable to postnatal neurogenesis or maturation of dormant, postmitotic precursors. Similar studies are lacking in the trigeminal ganglion (TG). Here we demonstrate by stereological methods that the number of neurons in the TG of adult male rats nearly doubles between the third and eighth months of age. The increase is mainly attributable to the addition of small, B-type neurons, with a smaller contribution of large, A-neurons. We looked for possible proliferative or maturation mechanisms that could explain this dramatic postnatal expansion in neuron number, using bromodeoxyuridine (BrdU) labeling, immunocytochemistry for neural precursor cell antigens, retrograde tracing identification of peripherally projecting neurons, and in vitro isolation of precursor cells from adult TG explant cultures. Cell proliferation identified months after an extended BrdU administration was sparse and essentially corresponded to glial cells. No BrdU-labeled cell took up the peripherally injected tracer, and only a negligible number coexpressed BrdU and the pan-neuronal tracer neuron-specific enolase. In contrast, a population of cells not recognizable as mature neurons in the TG and neighboring nerve expressed neuronal precursor antigens, and neural crest glioneuronal precursor cells were successfully isolated from adult TG explants. Our data suggest that a protracted maturation process persists in the TG that can be responsible for the neuronal addition found in the adult rat.

show abstract

The optical rotator

Cited by 113 publications

References 12 publications

The Causal Role of IL-4 and IL-13 inSchistosoma mansoniPulmonary Hypertension

The Causal Role of IL-4 and IL-13 inSchistosoma mansoniPulmonary Hypertension

Schistosomiasis-Induced Experimental Pulmonary Hypertension

Primary Sensory Neuron Addition in the Adult Rat Trigeminal Ganglion: Evidence for Neural Crest Glio-Neuronal Precursor Maturation

Contact Info

Product

Resources

About