The Ontogeny of Blood Cells, Complement, and Immunoglobulins in 3- to 12-Week-Old 15I5-B Congenic White Leghorn Chickens

Abstract

Six partially developed line 15I5-B congenic White Leghorn chicken strains were used to investigate age and B haplotype effects on hematocrit, total and differential leukocyte counts, hemolytic complement, immunoglobulins, and body weights. The .C-12 line had the lowest hematocrit values throughout the study. The .15I-5 line always had the highest mean hematocrit value and differed from the .C-12 and .P-13 lines at 3, 8, and 12 weeks, as well as lines .N-15 and .6-2 at 12 weeks. Ontogenically, the total leukocyte, hemolytic complement, immunoglobulins, and body weight values increased after 3 weeks in all lines. Lymphocytes were the predominant leukocyte after 3 weeks in all lines studied. At 12 weeks, the .C-12 line had the highest total leukocytes, heterophils, lymphocytes, and monocytes among the lines studied. The .6-2 line had a significantly higher hemolytic complement level than the .P-13 or the .C-12 line at all 3 ages, and at 12 weeks it also differed from the .7-2 line. The lines were found to be comparable for immunoglobulin classes M and G. In this study the line 15I5-B congenic lines differ for the traits of hematocrit and hemolytic complement level, and the results suggest the difference may be determined by genes in the B complex.

Molecular analysis reveals MHC class I intra-locus recombination in the chicken

“…Traditional serology, skin graft rejection, mixed lymphocyte reactivity, B haplotype association with disease resistance as well as restriction fragment length polymorphisms (RFLP) and DNA sequence analysis have identified extensive functional and physical polymorphisms in the loci encoding the class I and class II antigen presenting glycoproteins (Shen et al 1984;Guillemot et al 1988; Bacon and Motta 1982;Zoorob et al 1993).…”

“…With the aid of these B typing reagents important inbred and B congenic chicken lines have been established (Stone 1975;Shen et al 1984;Abplanalp 1992). These well-characterized inbred and B congenic chicken lines are important resources for the ongoing molecular analysis of the B complex.…”

Functional analysis of avian class I (BFIV) glycoproteins by epitope tagging and mutagenesis in vitro

“…Chickens used were from either the inbred RPRL lS.B congenic lines [24] or the non-inbred line 0 [25,261 developed at the USDA Avian Disease and Oncology Lab in East Lansing, Michigan. The congenic lines are 99.9 % identical to the inbred 1515 line, but differ at the MHC.…”

Analysis of Restriction Fragment Length Polymorphisms of the Major Histocompatibility Complex of 15I5-B-Congenic Chicken Lines

“…The origin of these lines has been described (Cole, 1968;Stone, 1975;Bacon and Motta, 1982;Shen et al, 1984). Line 15Is was used as the background line because chickens within this line are nearly histocompatible, are highly susceptible to Marek's disease (MD) and subgroups A and B avian leukosis virus, and are progressors of Rous' sarcoma virus tumors.…”

“…The 15Is B-congenic lines have been evaluated for many aspects of immune response and disease resistance during at least 10 generations of backcrossing and after the establishment of homozy- gous lines. The lines have been studied for hematocrits, hemolytic complement levels, BW, total leukocyte and differential blood cell types, immunoglobulin M and G levels (Shen et al, 1984), developmental antigens (Qureshi et al, 1986b), phagocytic function of peritoneal macrophages (Qureshi et al, 1986a), RSV tumor regression , lymphoid leukosis , coccidiosis (Lillehoj et al, 1989), MD resistance (Bacon, 1987), and antibody responses to sheep red blood cells, Brucella abortus antigen, and infectious bursal disease virus vaccine (Bacon et al, 1987).…”