The Olive Constituent Oleuropein Exhibits Proteasome Stimulatory Properties <i>In Vitro</i> and Confers Life Span Extension of Human Embryonic Fibroblasts

Katsiki, Magda; Chondrogianni, Niki; Chinou, İoanna; Rivett, A. Jennifer; Gonos, Efstathios S.

doi:10.1089/rej.2006.0513

Cited by 172 publications

(164 citation statements)

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“…5 oxidant activity of OLE was also determined in human leukemic cell line (Jurkat) (Fares et al, 2011). Again, OLE decreased the intracellular levels of reactive oxygen species (ROS) in human embryonic fibroblasts and H 2 O 2 -exposed in insulin secreting β-cells (Katsiki et al, 2007;Cumaoglu et al, 2011). Thus, OLE could modulate the PM-induced genetic and oxidative damage by preventing free radical generation or by stimulating components of the antioxidant defense system.…”

Section: Discussionmentioning

confidence: 96%

Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes

Türkez

Toğar

2011

J. Toxicol. Sci.

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-The aim of this study was to investigate the protective effects of olive leaf extract (OLE) on genotoxicity and oxidative damage in cultured human blood cells treated with permethrin (PM) in the presence of a rat liver S9 mix containing cytochrome P 450 enzymes. Anti-genotoxic activities of OLE were studied using sister chromatid exchange (SCE) and chromosome aberration (CA) tests and furthermore total antioxidant capacity (TAC) and total oxidative status (TOS) were examined to determine the oxidative damage. Our results clearly revealed that treatment with PM (200 mg/l) alone increased SCE and CA rates and TOS level, decreased TAC level in cultured human blood cells. The OLE alone at the all tested doses did not induce any significant changes in the genotoxicity endpoint. However OLE leads to increases of plasma TAC level in vitro. OLE starts showing this positive effect at 100 mg/l. The combined treatment showed significant improvements in cytogenetic and biochemical parameters tested. Moreover, this improvement was more pronounced in the group received the high dose of the OLE. It could be concluded that the ethanol extract of OLE induced its genoprotective effect via the increase in the antioxidant capacity, inhibition of oxidative stress and scavenging of free radicals.

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Section: Discussionmentioning

confidence: 96%

Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes

Türkez

Toğar

2011

J. Toxicol. Sci.

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“…Vitamin E (Packer et al, 1974), kinetin Clark, 1994), carnosine (McFarland andHolliday, 1994) and garlic (Svendsen et al, 1994) are few examples of natural compounds that have been shown to exert a pro-longevity effect on human primary cultures. More importantly, natural substances that possess proteasome activating properties like oleuropein (Katsiki et al, 2007) have been also shown to promote lifespan extension. Given these findings, we sought to investigate the role of QUER, a potent, natural anti-oxidant, on survival and lifespan of human fibroblasts along with its possible effects on the main secondary antioxidant mechanism, the proteasome.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…It is responsible for the cellular clearance from damaged and oxidized proteins that may cause deleterious side effects upon lack of proteolysis and aggregation. Given that we have already shown that the proteasome can be activated either through genetic means or through natural means (Katsiki et al, 2007) endowing the cells with an enhanced survival capacity against oxidative stress and an extended lifespan, we have examined whether QUER as a potent antioxidant agent may stimulate the proteasome function. We show that CT-L proteasome activity and content are induced upon QUER treatment in HFL-1 human primary cells.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

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Anti-ageing and rejuvenating effects of quercetin

Chondrogianni

Kapeta

Chinou

et al. 2010

Experimental Gerontology

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180

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Homeostasis is a key feature of the cellular lifespan. Its maintenance influences the rate of ageing and it is determined by several factors, including efficient proteolysis. The proteasome is the major cellular proteolytic machinery responsible for the degradation of both normal and damaged proteins. Alterations of proteasome function have been recorded in various biological phenomena including ageing and replicative senescence. Proteasome activities and function are decreased upon replicative senescence, whereas proteasome activation confers enhanced survival against oxidative stress, lifespan extension and maintenance of the young morphology for longer in human primary fibroblasts. Several natural compounds possess anti-ageing/anti-oxidant properties. In this study, we have identified quercetin (QUER) and its derivative, namely quercetin caprylate (QU-CAP) as a proteasome activator with antioxidant properties that consequently influence cellular lifespan, survival and viability of HFL-1 primary human fibroblasts. Moreover, when these compounds are supplemented to already senescent fibroblasts, a rejuvenating effect is observed. Finally, we show that these compounds promote physiological alterations when applied to cells (i.e. whitening effect). In summary, these data demonstrate the existence of naturally occurring anti-ageing products that can be effectively used through topical application.

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“…Oleuropeine showed positive effect on 20S proteasome activity and on senescence of embryonic human fibroblasts. Although the activation rate was only of 1.2 fold, it was the first study to suggest that protea some activators might have the potential to be developed into anti-aging agents [24]. Our work attempts to enhance this theory.…”

Section: Discussionmentioning

confidence: 96%

Effect of -Lipoic Acid on Proteasomal Induction: Protection against Oxidative Damage in Human Skin Fibroblasts Cell Line NHDF

Sikdar¹,

Papadopoulou²,

Dubois³

2017

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As human skin is daily exposed to oxidative stress causing various unesthetical abnormalities, the road to effective anti-aging substances is being widely investigated. 20S proteasome is a key pathway in the breakdown of oxidized proteins. But its activity declines dramatically in aging cells. Nrf2 inducers α-lipoic acid (LA) and sulforaphane (SFN) have been described in the dietary industries for their antioxidant effects on various cell lines. However, since little is yet known about LA's capacity to protect skin cells from premature and extrinsic aging; our aim was to demonstrate the beneficial effect of LA on the cellular detoxification systems. On this purpose, we evaluated its effects against injuries induced by H 2 O 2 in NHDF and its likely positive effect on the chymotrypsin-like (CT-like) activity of 20S proteasome, using SFN as a reference. The cellular content in proteins was measured, as well as the production of Reactive Oxygen Species (ROS). Also, the induction of the proteasomal protein expression was investigated. The results show that after 48 h treatment, LA significantly decreased the percentage of ROS positive cells. Also, LA decreased the level of H 2 O 2 -induced carbonylated proteins and increased the proteasomal activity. Furthermore, LA upregulated the expression of the 20S proteasome β-subunit responsible for the CT-like activity (PSMB5).Overall, both molecules enhanced cell proliferation over 8 days. So, our investigation found evidence of the higher capacity of LA to induce 20S proteasome activity with less toxicity in human fibroblasts compared to reference molecule SFN. These results tend to demonstrate that the induction of the proteasomal activity might be a part of the antioxidant potential of LA. To our knowledge, this is the first study to elucidate the capacity of LA to activate

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The Olive Constituent Oleuropein Exhibits Proteasome Stimulatory Properties In Vitro and Confers Life Span Extension of Human Embryonic Fibroblasts

Cited by 172 publications

References 50 publications

Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes

Olive (Olea europaea L.) leaf extract counteracts genotoxicity and oxidative stress of permethrin in human lymphocytes

Anti-ageing and rejuvenating effects of quercetin

Effect of -Lipoic Acid on Proteasomal Induction: Protection against Oxidative Damage in Human Skin Fibroblasts Cell Line NHDF

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