2003
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Abstract: Many commercial laboratory diets have detectable levels of isoflavones (e.g., phytoestrogens such as genistein [GN]) that have weak estrogenic activity both in vitro and in vivo. During validation studies of the uterotrophic bioassay, diet samples from 20 participating laboratories were collected and analyzed for three major phytoestrogens: GN, daidzein (DN), and coumestrol (CM). Soy phytoestrogens GN and DN were found at total phytoestrogen levels from 100 to 540 microg/g laboratory diet; a forage phytoestrog… Show more

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Cited by 74 publications
(64 citation statements)
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References 59 publications
(104 reference statements)
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“…In 1997 the Organization for Economic Cooperation and Development (OECD) initiated a high priority activity to develop new and revised guidelines for screening and testing the estrogenicity of potential endocrine disrupters (OECD, 1998). One of the validated screening was the uterotrophic bioassay, which demonstrated the uterine growth of pre-pubertal or ovariectomized rats by following E2 or genistein treatment (i.e., gavage or intraperitoneal injection) (Owens et al, 2003;Naciff et al, 2004;Ashby and Odum, 2004).…”
Section: Fl Fl Fl Fl Fla a A A Av V V V Vonoid Estr Onoid Estr Onoid mentioning
confidence: 99%
“…In 1997 the Organization for Economic Cooperation and Development (OECD) initiated a high priority activity to develop new and revised guidelines for screening and testing the estrogenicity of potential endocrine disrupters (OECD, 1998). One of the validated screening was the uterotrophic bioassay, which demonstrated the uterine growth of pre-pubertal or ovariectomized rats by following E2 or genistein treatment (i.e., gavage or intraperitoneal injection) (Owens et al, 2003;Naciff et al, 2004;Ashby and Odum, 2004).…”
Section: Fl Fl Fl Fl Fla a A A Av V V V Vonoid Estr Onoid Estr Onoid mentioning
confidence: 99%
“…First, DBP does not display binding affinity for the rat uterine estrogen receptor, i.e., there is no displacement of bound [ 3 H]17β-estradiol at concentrations up to 1 mM concentrations in vitro (Blair et al 2000). Second, in vivo toxicological studies, with some including gene activation profiles, indicate that DBP does not elicit responses indicative of an estrogen mode of action (Ema et al 2000;Ema and Miyawaki 2001;Mylchreest et al 1998Mylchreest et al , 1999Schulz et al 2001;Zacharewski et al 1998). A single data set that included data for all four standardized protocols was judged adequate for the negative chemical to conserve resources and animals.…”
Section: Design Of Phase 2 Coded Single-dose Studiesmentioning
confidence: 99%
“…According to OECD post validation recommendations, laboratories conducting the uterotrophic bioassay for either research, or regulatory purposes may routinely use diets containing levels of phytooestrogens <325-350 µg/g of total genistein equivalents without impairing the responsiveness of the bioassay (24). However, this is contraindicated by current literature, which specifically states that diets containing less than this value still have the potential to alter the results of vaginal opening and uterotrophic assay (29).…”
Section: Gpmentioning
confidence: 99%