The Number of FoxP3-positive Cells and Their Contacts with Tryptase-positive Mast Cells Increase in Tape-stripped Skin that Does not Develop the Isomorphic Psoriatic Reaction

Suttle, Mireille-Maria; Ritanen, Riikka-Liisa; Harvima, Ilkka T.

doi:10.2340/00015555-3023

Cited by 4 publications

(9 citation statements)

References 14 publications

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“…After xation of cryosections in ice-cold acetone for 10 min, FoxP3 was stained immunohistochemically using 10 µg/ml mouse monoclonal anti-human FoxP3 antibody (clone 236A/E7) (Abcam, Cambridge, UK), and the bound mAb was visualized with the avidin-biotin-peroxidase (ABC) technique using the Vectastain Elite ABC kit (Vector Laboratories, Burlingame, CA) together with 0.05% 3,3'-diaminobenzidine tetrahydrochloride, 0.04% nickel chloride and 0.03% hydrogen peroxide [10]. Unrelated mouse IgG was used as the control.…”

Section: Histochemical Staining Methodsmentioning

confidence: 99%

“…The double-staining method for tryptase + and FoxP3 + cells has previously been developed [10]. Brie y, cryosections were xed in cold acetone for 10 min followed by blocking with diluted normal horse serum.…”

Section: Histochemical Staining Methodsmentioning

confidence: 99%

“…Four mm punch biopsies were taken after local anesthesia with lidocaine-adrenalin from the lesional and nonlesional (about 2 cm apart from the lesion) skin at varying body sites. The biopsies were immediately embedded in OCT compound (Miles Scienti c, Naperville, IL) and frozen for preparing 5-µm-thick cryosections [4,10].…”

Section: Patientsmentioning

confidence: 99%

“…In a variety of experimental models, the interaction of Tregs with mast cells has been found to be one essential mechanism for immunosuppression, and interleukin-9 from Tregs has been considered to be the essential mediating cytokine [7][8][9]. In addition, this cellular interaction may be involved in the isomorphic psoriatic lesion, i.e., in the Köbner reaction, through prevention of its development [10]. Therefore, the double-staining technique previously developed for demonstrating tryptase + mast cells and FoxP3 + cells on the same cryosection [10] was utilized in this study to examine whether these cells interact in BCC.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, this cellular interaction may be involved in the isomorphic psoriatic lesion, i.e., in the Köbner reaction, through prevention of its development [10]. Therefore, the double-staining technique previously developed for demonstrating tryptase + mast cells and FoxP3 + cells on the same cryosection [10] was utilized in this study to examine whether these cells interact in BCC. The results show a high increase in the number of these cells and in apparent morphological contacts (AMCs) between them in BCC.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

FoxP3-positive cells and their contacts with mast cells are highly increased in basal cell carcinoma

Kaukinen

Harvima

2023

Preprint

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The cells of the immune system are thought to contribute to the development of skin cancers through immune evasion. One possible mechanism for this can be the interaction between mast cells and regulatory T cells (Tregs). Therefore, fresh frozen biopsies from the lesional and nonlesional skin of 16 patients with basal cell carcinoma (BCC) were processed for the enzymehistochemical staining of mast cell tryptase, immunohistochemical staining of FoxP3 as well as for the double-staining method to label tryptase+ cells and FoxP3+ (a marker of Tregs) cells on the same cryosection. The cell numbers and apparent morphological contacts (AMCs) between these cell types were counted. The results show a high increase in the number of tryptase+ cells, FoxP3+ cells and AMCs between them in the lesional compared to corresponding nonlesional skin (p<0.0001). Therefore, a theoretical morphological basis is present in the BCC lesion for permitting an immune evasive microenvironment.

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Section: Histochemical Staining Methodsmentioning

confidence: 99%

Section: Histochemical Staining Methodsmentioning

confidence: 99%

Section: Patientsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

FoxP3-positive cells and their contacts with mast cells are highly increased in basal cell carcinoma

Kaukinen

Harvima

2023

Preprint

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Increased expression of complement C3c, iC3b, and cells containing CD11b or CD14 in experimentally induced psoriatic lesion

Rahkola,

Harvima,

Harvima

2024

Clinical and Experimental Immunology

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Psoriasis is a chronic inflammatory skin disease with a characteristic isomorphic reaction, i.e., the Köbner reaction, induced by slight epidermal trauma. In this study, the tape-stripping technique was used to induce the development of Köbner reaction in 18 subjects with psoriasis. Eight subjects developed the positive reaction. To study the early cellular changes, skin biopsies were taken at the baseline and subsequent time points of 2 hours, 1 day, 3 days and 7 days for the immunostaining of complement C3c, iC3b, and cells expressing complement receptor 3 (CD11b/CD18; a receptor of iC3b) or CD14. The results show that the positive Köbner reaction associated with rapid (2h-1d) and sustained (3-7d) increase in the expression of epidermal C3c and iC3b and dermal C3c. In addition, there was a positive correlation between CD11b+ and CD14+ cells in baseline and 2h-1d biopsies with a subsequent increase in CD11b+ and CD14+ cells in 3-7d biopsies in the Köbner-positive group. In the Köbner-negative group, only a transient increase in epidermal iC3b at 2h-1d, as well as rapid (2h-1d) and sustained increase (3-7d) in dermal iC3b and CD14+ cells, was observed. In experiments with cultured monolayer keratinocytes, a slight cell damage already at 30 mJ/cm2 ultraviolet B irradiation led to increased expression of C3c, but not iC3b. Therefore, there are marked differences between Köbner groups in respect to the expression of C3c, iC3b and cells expressing CD11b or CD14. Of note is the rapid and sustained increase in epidermal C3c and iC3b in the positive Köbner reaction.

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FoxP3-Positive Cells and Their Contacts with Mast Cells Are Highly Increased in Basal Cell Carcinoma

Kaukinen,

Harvima,

Harvima

2023

Int Arch Allergy Immunol

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Introduction: The cells of the immune system are thought to contribute to the development of skin cancers, such as basal cell carcinoma (BCC). One possible mechanism may be the interaction between mast cells and regulatory T cells (Tregs), resulting in immunosuppression. Methods: Fresh-frozen biopsies from the lesional and nonlesional skin of 16 patients with BCC were processed for the enzymehistochemical staining of mast cell tryptase, immunohistochemical staining of FoxP3 (a marker of Tregs) as well as for the double-staining method to label tryptase+ cells and FoxP3+ cells on the same cryosection. The cell numbers and apparent morphological contacts (AMCs) between these cell types were counted. Results: There was a high increase in the number of tryptase+ cells, FoxP3+ cells, and AMCs between them in the lesional compared to corresponding nonlesional skin (p < 0.0001) in all cases. Conclusion: A morphological basis is theoretically present in BCC, suggesting an immune evasive microenvironment.

show abstract

The Number of FoxP3-positive Cells and Their Contacts with Tryptase-positive Mast Cells Increase in Tape-stripped Skin that Does not Develop the Isomorphic Psoriatic Reaction

Cited by 4 publications

References 14 publications

FoxP3-positive cells and their contacts with mast cells are highly increased in basal cell carcinoma

FoxP3-positive cells and their contacts with mast cells are highly increased in basal cell carcinoma

Increased expression of complement C3c, iC3b, and cells containing CD11b or CD14 in experimentally induced psoriatic lesion

FoxP3-Positive Cells and Their Contacts with Mast Cells Are Highly Increased in Basal Cell Carcinoma

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