The novel primers for sex identification in the brown eared‐pheasant and their application to other species

Wang, N; Zhang, Zhenwu

doi:10.1111/j.1755-0998.2008.02177.x

Cited by 36 publications

(30 citation statements)

References 4 publications

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“…We pulled 2–3 body feathers from each bird in 2008 and 2009. DNA was extracted from the pulp of feathers and molecular techniques were used to sex the birds [25].…”

Section: Methodsmentioning

confidence: 99%

Three-Phase Fuel Deposition in a Long-Distance Migrant, the Red Knot (Calidris canutus piersmai), before the Flight to High Arctic Breeding Grounds

Hua

Piersma²,

2013

PLoS ONE

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Refuelling by migratory birds before take-off on long flights is generally considered a two-phase process, with protein accumulation preceding rapid fat deposition. The first phase expresses the demands for a large digestive system for nutrient storage after shrinkage during previous flights, the second phase the demands for fat stores to fuel the subsequent flight. At the last staging site in northward migration, this process may include expression of selection pressures both en route to and after arrival at the breeding grounds, which remains unascertained. Here we investigated changes in body composition during refuelling of High Arctic breeding red knots (Calidris canutus piersmai) in the northern Yellow Sea, before their flight to the tundra. These red knots followed a three-phase fuel deposition pattern, with protein being stored in the first and last phases, and fat being deposited mainly in the second phase. Thus, they did not shrink nutritional organs before take-off, and even showed hypertrophy of the nutritional organs. These suggest the build up of strategic protein stores before departure to cope with a protein shortage upon arrival on the breeding grounds. Further comparative studies are warranted to examine the degree to which the deposition of stores by migrant birds generally reflects a balance between concurrent and upcoming environmental selection pressures.

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“…We pulled 2–3 body feathers from each bird in 2008 and 2009. DNA was extracted from the pulp of feathers and molecular techniques were used to sex the birds [25].…”

Section: Methodsmentioning

confidence: 99%

Three-Phase Fuel Deposition in a Long-Distance Migrant, the Red Knot (Calidris canutus piersmai), before the Flight to High Arctic Breeding Grounds

Hua

Piersma²,

2013

PLoS ONE

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“…For determination of sex of monomorphic species of birds, primers Sex1 (5'CTCCCAAGGATGAGAAACTGT-GCAAAACAGGTA 3') and Sex2 (5'CCTTCACTTCCAT-TAAAGCTGATCTGGAATTTC 3') which amplify CHD-Z and CHD-W genes fixed on sex genes (Wang & Zhang 2009), were used.…”

Section: Methodsmentioning

confidence: 99%

The first report of nine species of haemosporidian parasites (Haemosporida: Haemoproteus, Plasmodium and Leucocytozoon) in wild birds from Slovakia

2012

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During 2009 and 2010 samples from 251 wild birds (37 species) from nine sites of Slovakia were collected. Blood samples were tested for Plasmodium, Haemoproteus, and Leucocytozoon spp. parasites using both PCR-based and microscopy techniques. The overall prevalence of infection was 56.6%, while prevalence of Haemoproteus a Plasmodium spp. (were evaluated separately from Leucocytozoon) was 46.6% and the prevalence of Leucocytozoon spp. was 33.9%. After microscopic examination of stained blood smears the identification of haemoproteids was performed. There were 14 species of three genera identified, i.e., Haemoproteus parabelopolskyi, H. belopolskyi, H. magnus, H. fallisi, H. fringillae, H. balmorali, H. dolniki, H. tartakovskyi, H. lanii, Leucocytozoon majoris, L. dubreuili, Plasmodium vaughani, P. relictum, P. circumflexum. Except for H. fringillae, H. balmorali, H. belopolskyi, L. majoris and L. dubreuili, the mentioned species of parasites were recorded for the first time in Slovakia.

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“…Since the sexing primers were established by Griffith et al (1998); Fridolfsson and Ellegren (1999), many other primers have been developed for sex identification. Based on a sexing report of Wang and Zhang (2009), they designed a primer pair sex1/sex2 from CHD genes of the Brown-eared Pheasant (Crossoptilon mantchuricum) and the primers can be used to sex many other pheasants accurately as well as some Passeriform species. Kasuga et al (2012) said that the W-and Z-linked EE0.6 sequences used for molecular sexing of captive Japanese crested ibis on Sado island.…”

Section: Disscussionmentioning

confidence: 99%

Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in Indonesia

Sulandart

Zein

2012

HAYATI Journal of Biosciences

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Visually identifying the sex of a bird can be difficult. It cannot be done in half the world's species when they are adults, and virtually none can be sexed as chicks. Despite this, the sex of a bird is vital for captive breeding. An increased number of birds are being sexed using DNA amplification techniques. In this approach, the CHD-W and CHD-Z are distinguished by the amplification of an intron present in both genes. PCR products on the gel electrophoresis vary in size revealing one band in males at the CHD-Z, and two bands in females corresponding to both the CHD-W and CHD-Z. Two independent sets of primer (P8/P2 and 2550F/2718R) were used to amplify the CHD gene region from both the Z and W chromosome. One hundred and ten (110) birds were sexed using first pair of primers: (P8/P2). Sexing results indicated that 81.8% were successfully determined, 12.7% failed to be amplified and 5.5% were not perfectly determined because the PCR products showed thick band. The thick band caused misidentified female to male birds. An alternative primer (2550F/2718R) was applied to solve the problem. Two hundreds and twenty-nine birds were sexed and the results showed 100% successfully determined. From this study, it is suggested to use a pair of 2550F and 2718R primers for distinguishing a male from a female bird.

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The novel primers for sex identification in the brown eared‐pheasant and their application to other species

Cited by 36 publications

References 4 publications

Three-Phase Fuel Deposition in a Long-Distance Migrant, the Red Knot (Calidris canutus piersmai), before the Flight to High Arctic Breeding Grounds

Three-Phase Fuel Deposition in a Long-Distance Migrant, the Red Knot (Calidris canutus piersmai), before the Flight to High Arctic Breeding Grounds

The first report of nine species of haemosporidian parasites (Haemosporida: Haemoproteus, Plasmodium and Leucocytozoon) in wild birds from Slovakia

Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in Indonesia

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