2017
DOI: 10.4172/2167-7921.1000238
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Abstract: To clarify how the osteoarthritis (OA)-induced catabolic factor interleukin (IL)-1β affects chondrocyte energy metabolism, and especially to define the downstream pathway linking nicotinamide adenine dinucleotide (NAD)-dependent deacetylase Sirtuin-1 (Sirt-1) to energy metabolism in OA chondrocytes. Human chondrocytes were isolated from articular cartilage samples of patients with OA. The level of energy metabolism of OA chondrocytes was evaluated by monitoring the activity of the energy metabolic sensor, aden… Show more

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Cited by 6 publications
(7 citation statements)
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“…Consequently, metabolic alterations resulting in leading to changes in the NADH/NAD+ ratio, have potential to indirectly impact the range of cellular processes controlled by Sirtuin proteins, such as mitochondrial biogenesis and insulin sensitivity, and which includes SirT1 activity. Interestingly, the loss of SIRT1 and the NAD + co-factor, are shown to be decreased in OA patients and experimental models of bone and joint disease [5][6][7][15][16][17] . Recent observational studies in mice suggest that loss of SIRT1 in all chondrocytes through use of the type II collagen promoter, predisposed to OA development at 1 year 18 .…”
Section: Discussionmentioning
confidence: 99%
“…Consequently, metabolic alterations resulting in leading to changes in the NADH/NAD+ ratio, have potential to indirectly impact the range of cellular processes controlled by Sirtuin proteins, such as mitochondrial biogenesis and insulin sensitivity, and which includes SirT1 activity. Interestingly, the loss of SIRT1 and the NAD + co-factor, are shown to be decreased in OA patients and experimental models of bone and joint disease [5][6][7][15][16][17] . Recent observational studies in mice suggest that loss of SIRT1 in all chondrocytes through use of the type II collagen promoter, predisposed to OA development at 1 year 18 .…”
Section: Discussionmentioning
confidence: 99%
“…Cartilage ECM breakdown products, fibronectin and hyaluronic acid (Okamura et al, 2001;Termeer et al, 2002;van Lent et al, 2012) and intracellular alarmins [HMGB1 (Li et al, 2011), S100 proteins (van Lent et al, 2012)] signal on synovial macrophages, synoviocytes, or chondrocytes promoting the local production of numerous inflammatory mediators [IL-1β (He et al, 2002), VEGF, TNFα, IL-6 (Ushiyama et al, 2003), PDGFs (Pohlers et al, 2006)]. Moreover, regulatory mechanisms involved in controlling NAD + levels have been demonstrated to be affected in OA because of an increased expression of IL-β and a compromised activity of NADases (Kobayashi et al, 2017). Thus, endoplasmic reticulum stress and highly expressed vacuoles in concert with the expression of prognostic OA marker IL-1βR1 highlight the occurrence of a strict correlation between OA-IFP biology and the inflammatory microenvironment (Daheshia and Yao, 2008).…”
Section: Discussionmentioning
confidence: 99%
“…These findings suggest that mechanical loading increases glucose uptake via an upregulated Glut1 in osteoblasts. This increase in glucose uptake may reduce the activity of the energy sensor SIRT1 as well as AMPK, since SIRT1 is known to crosstalk with AMPK, forming a SIRT1-AMPK positive feedback loop in cellular energy metabolism [23,50,51]. From the results of previous studies, we postulated that the mechanical loading could induce the increased level of Glut-1 and its resultant decreases in both energy sensors, SIRT1 and AMPK.…”
Section: Mechanical Stressmentioning
confidence: 95%
“…Articular cartilage explants were cut into small pieces, washed with PBS, and digested individually with 1.0 mg/mL collagenase type I (#035-17604, Fujifilm Wako Pure Chemical Inc., Tokyo, Japan) in DMEM (Sigma-Aldrich) overnight on a shaking platform at 3 • C. Isolated chondrocytes from each explant were collected following centrifugation; washed three times with PBS; resuspended; and then cultured in DMEM supplemented with 10% heat-inactivated FBS, 2 mM L-glutamine (Sigma-Aldrich), and 100 U/mL each of penicillin and streptomycin (Cosmo Bio) at 3 • C in a humidified atmosphere of 95% air and 5% CO 2 , as previously reported [23,30,57]. A sufficient number of cultured chondrocytes were obtained from the relatively normal parts of surgically resected cartilage tissues.…”
Section: Monolayer Human Cell Cultures: Chondrocytes and Osteoblastsmentioning
confidence: 99%
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